Curriculum Vitae: Dr. Scott Lohrke, PhD, Microbiology

United States Government Security Clearances:

1. Secret - Status: Active, Effective April 16, 2010

2. Top Secret - Active, Effective May 28, 2010

Citizenship: USA

Work Experience:

1. Bigelow Aerospace Advanced Space Studies

4640 South Eastern Avenue, Las Vegas, NV, 89119

Title: Senior Microbiologist

Supervisor: Dr Colm Kelleher

02/2009-06/2010

Project/Duties: Lead biological senior scientist at BAASS. The primary

duties involve responsibility for the development of laboratory

capabilities for the evaluation of novel aerospace technologies

including novel materials and propulsion systems for possible

detrimental effects on humans and the environment. Additional duties

involve the development of research protocols and techniques in

support of ongoing research projects and oversight of daily science

operations, which includes supervision of BAASS technicians. The

project required employees to obtain government security clearances

which preclude precise discussion of objectives.

2. SAIC

Naval Health Research Center

Detachment Environmental Health Effects Laboratory

2729 R Street, Building 837, Area B, Wright-Patterson Air Force Base,

OH

Title: Life Science Manager I, Environmental Toxicology

Supervisor: Kevin Kaiser

01/03-12/2008

Project/Duties: Lead senior scientist at NHRC/EHEL in the Cellular and

Molecular Biology Department. The main focus was the development and

submission of new initiatives and proposals for external research

funding at NHRC/EHEL (Significant highlight: total external funds

acquired $1,300,000). Primary research objectives involve issues

dealing with environmental microbiology and toxicology. (Additional

highlights were the development of a microbiology capability where

none existed previously, and coauthor on two peer reviewed

publications and primary author on three technical reports). Other

research objectives include investigations of the effects of exposure

to a range of chemicals at the molecular and genetic level.

Responsible for the development of research protocols and techniques

in support of ongoing research projects and oversight of daily lab

operations, which includes supervision of SAIC technicians at

NHRC/EHEL.

External Funded Projects:

A. Development of a specific and sensitive biological sensor for

microbial agents employing a partial complement fixation

reaction. ($350,000 three years- Office of Naval Research)

B. Identification of promoters induced by specific hazardous

chemicals utilizing a version of In Vivo Expression Technology

(IVET). Possible application for the development of a bacterial-

based biosensor able to detect and quantify specific chemicals

in the environment. ($650,000 three years - Office of Naval

Research)

C. Application of Bromodeoxyuridine labeling to identify the

presence of nonculturable, metabolically active microorganisms

in diverse environmental samples. ($300,,000 two years - Office

of Naval Research)

3. Sustainable Agricultural Systems Laboratory

Beltsville Agricultural Research Center, Beltsville, Md.

Title: Post Doctoral Research Associate

Supervisor: Dr. Daniel Roberts

09/2000-12/2002

Project: Identification and characterization of traits in the plant

growth promoting bacterium Enterobacter cloacae important in seed and

root colonization and suppression of "damping off" of cucumber caused

by Pythium ultimum. Isolation and screening of potential biocontrol

agents of damping-off, take-all disease of wheat, and root-knot

nematodes. Also responsible for the day to day operation of the

laboratory. (Significant highlight: Authored or co-authored nine peer

reviewed scientific articles).

4. Department of Molecular Genetics and Microbiology

University of Florida

Title: Post Doctoral Research Associate

Supervisor: Dr. Shouguang Jin

08/1999-08/2000

Project: Isolation, identification and characterization of ancillary

genes involved in virulence gene expression in Agrobacterium

tumefaciens. Investigations into the mechanism of interactions between

the bacterial sensor protein VirA and plant derived phenolic inducers.

Reconstitution of plant phenolic inducible virulence gene expression

in the heterologous host Escherichia coli. (Significant highlight:

Authored or co-authored two peer reviewed scientific articles).

5. Department of Immunology and Microbiology

University of Arkansas for Medical Sciences

Title: Post Doctoral Research Associate

Supervisor: Dr Shouguang Jin

11/1997-08/1999

Project: Isolation, identification and characterization of ancillary

genes involved in virulence gene expression in Agrobacterium

tumefaciens. Investigations into the mechanism of interactions between

the bacterial sensor protein VirA and plant derived phenolic inducers.

Reconstitution of plant phenolic inducible virulence gene expression

in the heterologous host Escherichia coli. Significant highlight was

developing and obtaining a patent - See Patents section below.

6. Department of Microbiology

National University of Ireland. Cork, Ireland

Title: Post Doctoral Research Associate

Supervisor: Professor Fergal O'Gara

10/1995-11/1997

The main emphasis was to serve as the liaison between the project

coordinator and the project partners. This involved maintaining

communication with 16 partners including university and private

sectors throughout Europe. Additional tasks involved ensuring that

partners conformed to regulations regarding genetically modified

organisms. The results obtained from each partner were integrated into

a technical report submitted to the regulatory authorities of the

European Commission on a yearly basis.

Scientific Projects:

1. Development and evaluation of wild type and genetically modified

Pseudomonas fluorescens biocontrol inoculants engineered for

overproduction of the antifungal metabolite 2,4-

diacetyphloroglucinol (DAPG).

2. Evaluation of effects of biocontrol strains on the indigenous

community of Rhizobium trifolii and Pseudomonas fluorescens in

soil.

3. Development and evaluation of a containment system in Rhizobium

meliloti for improved plasmid maintenance based on conditional

expression of thyA (thymidylate synthase).

7. Children's Hospital and Medical Center

Department of Infectious Disease. Seattle, Washington

Title: Research Technician

Supervisor: Dr. Arnold Smith

Project: Molecular biology of pillin production in Haemophilus

influenzae

8. University of Washington

Department of Oral Biology.

Title: Research Technician

Supervisors: Dr. Eileen Watson and Dr. Bernard Moncla

Project: Regulation of lactose metabolism in Actinomyces viscosus

9. University of Chicago

Department of Biochemistry and Molecular Biology

Title: Research Technician

Supervisor: Dr. Kan Agarwall

Project: Molecular biology of gastrin hormone.

10. University of Illinois at Chicago

Department of Genetics

Title: Research Technician

Supervisor: Dr. Igor Roninson

Project: Molecular biology of multiple chemotherapy drug resistance.

Patents:

1. A novel mechanism for expression of genes in heterologous hosts.

Inventors: Scott M. Lohrke, Shouguang Jin (University of Florida),

Marie Chow (University of Arkansas for Medical Sciences) and

Konstantin Severinov (Rutgers University) U.S. Patent 6764837 issued

on July 20, 2004.

Peer Reviewed Publications:

1. Lohrke, S.M., McKenna, L, Dery, P.D., and Roberts, P.D. Mutation of

degS, encoding a putative periplasmic serine protease in Enterobacter

cloacae decreases seed and root colonization, but does not affect

biocontrol of damping-off caused by Pythium ultimum on cucumber. (In

preparation).

2. Roberts, D.P., McKenna, L.F., Lohrke, S.M., Rehner, S and de Souza,

J.T.. Pyruvate

dehydrogenase activity is important for colonization of seeds and

roots by Enterobacter cloacae. Soil Biol. Biochem. 39: 2150-2159.

(2007).

3. Liu, S., Hu, X., Lohrke, S.M., Baker, C.J., Buyer, J.S., de Souza,

J.T., and Roberts, D.P. 2007. Role of sdhA and pfkA and catabolism of

reduced carbon during colonization of cucumber roots by Enterobacter

cloacae. Microbiology. 153: 3196-3209.

4. Roberts, D.P., McKenna, L.F., Hu, X., Lohrke, S.M., Kong, H.S.,

deSouza, J.T., Baker, C.J. and Lydon, J. Mutation in cyaA in

Enterobacter cloacae decreases cucumber root colonization. Arch

Microbiol. 187: 101-115. (2007).

5. Arfsten D.P., Bekkedal, M., Wilfong, E.W.. Rossi, J., Grasman, K.A.,

Healy, L.B., Rutkiewicz, J.M., Johnson, E.W., Thitoff, A.R., Jung,

A.E., Lohrke, S.M., Sheffer, D.J., and Still, K.R. Study of the

reproductive effects in rats surgically implanted with depleted

uranium for up to 90 days. J. of Toxicology and Environmental Health,

Part A, 68: 967-997. (2005)

6. Roberts, D.P., Lohrke, S.M., S.L.F. Meyer, J.S. Buyer, J.H. Bowers,

C.J. Baker, W. Li, J. de Souza, J.A. Lewis, and S. Chung. Biocontrol

agents applied individually and in combination for suppression of

soilborne diseases of cucumber. Crop Protection 24:141-155. (2005)

7. Arfsten, D.P., Johnson, E.W., Thitoff, A.R., Jung, A.E., Bausman, T.A.

Eggers, J.S., Lohrke, S.M., E.R. Wilfong, E.R., and Bobb, A.J.

Impact of 30-day oral dosing with N-Acetyl-L-Cysteine on Sprague-

Dawley rat physiology. Int J. Toxicol. 23: 239-247. (2004)

8. Roberts, D.P., Lohrke, S.M., Buyer, J.S., Baker, C.J., and Liu, S.

Colonization of subterranean plant surfaces and suppression of

soilborne plant pathogens: Studies with Enterobacter cloacae. Recent

Res. Devel. Microbiology. 7: 161-174. (2003)

9. Roberts, D.P., and Lohrke, S.M. United States Department of

Agriculture - Agricultural Research Service research programs in

biological control of plant diseases. Pest Manag. Sci. 59: 654-664.

(2003)

10. Walsh, U.F., Mo nne-Loccoz, Y., Tichy, H.-V., Gardner, A., Corkery,

D.M., Lohrke, S., and O'Gara, F. Residual impact of the biocontrol

inoculant Pseudomonas fluorescens F113 on the resident populations of

rhizobia nodulating a red clover rotation crop. Microbial Ecol. 45:

145-155. (2003)

11. Stromberg, E.L., Roberts, D.P., Lacy, G.H., Lohrke, S.M., Li, W., and

Buyer, J.S. Field evaluation of bacterial isolates and seed treatment

fungicides for the control of take-all in Virginia, 2001. Biological

and Cultural Tests for Control of Plant Diseases. (2002).

12. Lohrke, S.M., Dery, P., Li, W., Reedy, R., Kobayashi, D., and Roberts,

D.P. Mutation of rpiA in Enterobacter cloacae decreases seed and root

colonization and biocontrol of damping-off caused by Pythium ultimum

on cucumber. Molecular Plant-Microbe Interactions. 15: 817-825 (2002).

13. Li, W., Roberts, D.P., Dery, P.D., Meyer, S.F., Lohrke, S.M., Lumsden,

R.D., and Hebbar, K.P. Broad spectrum anti-biotic activity and disease

suppression by the potential biocontrol agent Burkholderia ambifaria.

Crop Protection. 21:129-135. (2002).

14. Lohrke, S.M., Yang, H., and Jin, S. Reconstitution of acetosyringone-

mediated Agrobacterium tumefaciens virulence gene expression in the

heterologous host Escherichia coli. Journal of Bacteriology. 183:

3704-3711. (2001).

15. Lohrke, S.M., Madrak, C.J., Hur, H.-G., Judd, A.K., Orf, J.H., and

Sadowsky, M.J. Inoculum density-dependent restriction of nodulation in

the soybean-Bradyrhizobium japonicum symbiosis. Symbiosis 29: 59-70.

(2000).

16. Lohrke, S.M., Nechaev, S., Yang, H., Severinov, K., and Jin, S.

Transcriptional activation of Agrobacterium tumefaciens virulence gene

promoters in Escherichia coli requires the rpoA gene from A.

tumefaciens encoding the alpha subunit of RNA polymerase. Journal of

Bacteriology. 181: 4533-4539. (1999).

17. Barea, J.M., Andrade, G., Bianciotto, V., Dowling, D., Lohrke, S.,

Bonfante, P., O'Gara, F., and Azcon-Aguillar, C. Impact on arbuscular

mycorrhiza formation of Pseudomonas strains used as inoculants for

biocontrol of soil-borne fungal plant pathogens. Applied and

Environmental Microbiology. 64: 2304-2307. (1998).

18. Lohrke, S.M., Day, B., Kumar-Kolli, V.S., Hancock, R., Yuen, J.P.-Y.,

DeSouza, M.L., Stacey, G., Carlson, R., Tong, Z., Hur, H.-G., Orf,

J.H., and Sadowsky, M.J. Bradyrhizobium japonicum noeD gene: A

negatively acting, genotype-specific nodulation gene for soybean.

Molecular Plant-Microbe Interactions. 11: 476-488. (1998).

19. Lohrke, S.M., Orf, J.H., and Sadowsky, M.J. Inheritance of host-

controlled nodulation restriction of Bradyrhizobium japonicum strain

USDA 110. Crop Science 36: 1271-1276. (1996).

20. Lohrke, S.M., Orf, J.H., Martinez-Romero, E., and Sadowsky, M.J. Host-

controlled restriction of nodulation by Bradyrhizobium japonicum

strains in serogroup 110. Applied and Environmental Microbiology. 61:

2378-2383. (1995).

21. Boundy-Mills, K.L., Kosslak, R.M., Tully, R.E., Pueppke, S.G., Lohrke,

S.M., and Sadowsky, M.J. Induction of the Rhizobium fredii nod box-

independent nodulation gene nolJ requires a functional nodD1 gene.

Mol. Plant-Microbe Interact. 7: 305-308. (1994).

22. Watson, E.L., Sodhi, S., Lohrke, S., and DiJulio, D.H. Glucose

stimulates cAMP accumulation in the oral bacterium Actinomyces

viscosus. Biochim. Biophys. Acta. 1178: 243-248. (1993).

23. Weber, A., Harris, K., Lohrke, S., and Smith, A.L. Inability to

express fimbriae results in impaired ability of Haemophilus influenzae

b to colonize the nasopharyx. Infect. Immun. 59: 4724-4728. (1991).

24. Xu, J., Lohrke, S., Hurlburt, I.H., and Hurlburt, R. Transformation of

Xenorhabdus nematophilus. Applied and Environmental Microbiology. 55:

806-812. (1989).

Technical Reports:

1. Lohrke, S.M. Doyle, T. and Chapman, G.D.. 2009. Impact of sodium

tungstate on the viability of selected microorganisms with differing

capacities for bioremediation. (Submitted).

2. Lohrke, S.M., Doyle, T., Arfsten, D., Wilfong, E. and Chapman, G.D.

2009. Identification of promoters induced by specific hazardous

chemicals utilizing a version of in vivo expression technology (IVET).

Possible application for the development of a bacterial based

biosensor able to detect and quantify specific chemicals in the

environment. (Submitted)

3. Lohrke, S.M., Wilfong, E., and Chapman, G.D. 2009. Development of a

specific and sensitive biological sensor for microbial agents

employing a partial complement fixation (Submitted).

Invited Presentations:

1. Lohrke, S., Mo nne-Loccoz, Y., McCarthy, J., Powell, J., Higgins, P.,

Joyce, H., and O'Gara, F. Experiences in the molecular ecology of the

rhizosphere with microbial releases. The 4th international symposium

on the biosafety results of field tests of genetically modified plants

and microorganisms. K. Oga (Ed.) Tsukuba, Japan, July 14-17, 1996.

2. Lohrke, S., H. Yang, H., and Jin, S. Reconstitution of acetosyringone

mediated Agrobacterium tumefaciens virulence gene expression in the

heterologous host Escherichia coli. 20th Annual Crown Gall Conference.

Houston, Texas. November 5-7, 1999.

Book/ Symposia chapters

1. Sadowsky, M.J., Lohrke, S.M., and Orf, J.H. Host-controlled

restriction of nodulation by Bradyrhizobium japonicum strain USDA 110

and characterization of the genes regulating nodulation. In: 2nd

European Nitrogen Fixation Conference and NATO Advanced Research

Workshop. Program and Abstracts, pp. 41. A. W jtowicz, J. Setkowska

and A. Szlagowska, eds. Scientific Publishers OWN, Polish Academy of

Sciences. Poznan, Poland. (1996).

2 Dunne, C., Delany, I., Fenton, A., Lohrke, S., Mo nne-Loccoz, Y., and

O'Gara. F. The biotechnology and application of Pseudomonas inoculants

for the biocontrol of phytopathogens. In: Biology of plant-microbe

interactions, pp. 441-448. G. Stacey, B. Mullin and P.M. Gresshof,

Eds. ISMPI, St. Paul, MN. USA. (1996).

3. Lohrke, S., Mo nne-Loccoz, Y., McCarthy, J., Powell, J., Higgins, P.,

Joyce, H., and O'Gara, F. Experiences in the molecular ecology of the

rhizosphere with microbial releases. In: Proceedings of the 4th

international symposium on the biosafety results of field tests of

genetically modified plants and microorganisms. K. Oga (Ed.) Tsukuba,

Japan, July 14-17. (1996).

4. O'Gara, F., Peruch, U., Barea, J.M., Nuti, M.P., Bonfante, P., Mo nne-

Loccoz, Y., McCarthy, J., Lohrke, S., and Powell, J. 1997. Enhancing

the potential of microbial inoculants through molecular microbial

ecology In: Biological fixation of nitrogen for ecology and

sustainable agriculture. H. Boethe, ed. Springer Verlag. (1997).

5. Dunne, C., Delany, I., Fenton, A., Aarons, S., Tola, E., Smith, L.,

Lohrke, S. and O'Gara, F. Molecular approaches for improved

biological control of plant diseases. In: Proceedings of the General

Assembly of the IOBC, Vienna, Australia, Bulletin Vol. 21(7), pp. 11-

20. (1998).

Education:

1. University of Minnesota

Minneapolis, Minnesota

Graduated: Ph.D. Microbiology

Thesis Title: Genetics of Host and Microsymbiont Affecting Nodulation

of Glycine max Plant Introduction Genotype PI 417566.

Advisor: Professor Michael Sadowsky

2. Indiana State University

Terre Haute, Indiana

Graduated: M.S. Microbiology

Thesis Title: Partial Characterization and Purification of Virus-Like

Particles from the Fungus Chlorophyllum molybdites.

Advisor: Professor Robert Bozarth

3. Marquette University

Milwaukee, Wisconsin

Graduated: B.S. Biology

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