Plant Assistant

Shail Shah

.Ph: (***) ***-****.**********@*****.***

Education:

Master of Science in Crop science G.P.A: 3.3 Texas Tech

University, Lubbock, TX

(Texas Tech Graduate student Scholarship 2009-2011)

Graduation: Aug 2011

Thesis defended in June, 2011

"Specialization: Cloned, sub-cloned, over-expressed, biochemically

characterized & performed mutagenesis studies on 3-? hydroxyl steroid

dehydrogenase from C.testosteronii."

Bachelor of Science in Biotechnology G.P.A: 3.8

Dr. D. Y. Patil University, Mumbai, India

Graduation: June

2009

Career profile:

> 2 yrs experience in molecular biology and genomics, 1.5 yrs experience

in cell culture and microbiology. Worked in a biopolymer facility for

1.5 yrs.

> Trained to work with rotary fermentors and in a sterile

environment(Beverages)

> Capable of developing SOPs, and analyzing/interpreting

biological/genomic data

Technical proficiency:

. Molecular biology-RT-PCR, PCR, DNA / RNA extraction, Western /

Southern blotting, gel electrophoresis, DNA sequencing and analysis,

Oligo/primer-design, site-directed mutagenesis, gene-transfection,

recombinant protein production, SDS-PAGE, molecular cloning and

expression profiling.

. Immunology-ELISA, affinity chromatography, column chromatography, thin

layer chromatography (TLC) and paper chromatography,

Immunohistochemistry, Immunocytochemistry, Immunoelectrophoresis,

Immunodiffusion

. Fermentation - Yeast Preparation, Nutrient addition, Sedimentation

. Microbiology-Gram staining, Capsule staining, M.P.N test, IMVIC tests

and Mycoplasma test.

. Sample Characterization- HPLC, Scanning Electron Microscopy, Fourier

Transform Electron Microscopy, BET Surface area and porosity analysis,

Supercritical Drying, Thermo gravimetric Analysis, XRD analysis, Gel

Permeation Chromatography

. Computer Skills:

Bioinformatics packages- Expasy, Vector NTI, Clustal W, BLAST, BLAT,

Swiss Pdbviewer, Emboss, Argus lab, Rasmol, Webgestal, Clustal X,

pyMOL

Bioinformatic databases: UCSC genome browser, UniProt, dbSNP, Galaxy

Software-Office, SAS, Sigma plot, Graphpad Prism, Calcusyn,

Biostatistics

Experience:

. Research Assistant: Texas Tech Dept of Plant and Soil Science, FBRI

Lubbock, TX; (June 2010-Aug 2011)

Thesis - Preparation of porous cellulose based biopolymer

materials as a basis for the development of a new light ultra

porous pure cellulose material called Aerocellulose or Aerogels.

Preliminary results showed that Aerogel materials made from

cellulose (Aerocellulose) possess very high impact strength,

very large surface area, and are lightweight. Regeneration of

cellulose from Plant material.

. Texas Tech University, Lubbock, TX; (Aug 2009 - Dec 2009)

Cloned, sub-cloned, over-expressed, biochemically characterized

& performed mutagenesis studies on 3-? hydroxyl steroid

dehydrogenase from C.testosteronii. Gained extensive experience

in designing primers, cloning vectors and expression systems.

. Research Intern, Dr. D. Y. Patil Institute for Biotechnology &

Bioinformatics, Mumbai, India; (Jan 2009 - June 2009)

Thesis - Characterization of ABC Transporter (Rv1747) Protein of

Mycobacterium Tuberculosis H37Rv.Worked on this transporter to

understand the modulation of its activity upon phosphorylation

by PKnF.We also tried to clone full length ABC transporter in

PVV16 Shuttle vector for In vivo further In-vivo studies.

. Research Trainee - Serum Institute Of India Research Foundation,

Pune, India; (Nov 2007-Dec 2007)

Learned about the formulation, manufacturing procedures & safety

issues involved in the production of various bacterial & viral

vaccines

. Research Trainee - Genome Biotechnologies Pvt. Ltd, Pune, India; (Jan

2007- Feb 2007)

In an industrial setting, performed bacterial culture, plasmid-

isolation, restriction-

digestion, molecular diagnosis of HIV proviral

DNA in simulated clinical samples using

recombinant DNA as the target and virus specific probes, primer

design, PCR, real-time PCR and data- analysis

. Chromous Biotech Pvt. Ltd, Mumbai, India (10/29-10/30/2007)

Attended workshop on "Cloning & Product Expression"

Presentations

. Poster; "Cellulose derived Biomaterials : A green strategy to obtain

economically favorable biocellulose based bio-materials" in the TTU

2011 Annual ICRC Council, Lubbock, TX; April 02011.Also won second

prize for the same at Graduate Research Poster Competition, Texas Tech

University, March 2011.

Extracurricular

. Represented Texas in American national chess tournament and won the

first prize(2010).

. USCF 1400 Rated Chess Player for United States Chess Federation.(2009-

present).

. Member of Big Brother- Big Sister foundation, Regular Blood Donator.

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