Quality Control Assistant
Resume:
Matthew R. Pocker
San Jose, CA. 95120
*******@*********.***
[pic]
Objective: To obtain a Research Associate or related position in the
Biotechnology/Pharmaceutical industry.
Education: Bachelor of Science, Biological Science
San Jose State University, 1998
Laboratory . Standard and Quantitative PCR . Construct generation
Skills: . Affymetrix Genechip Arrays
. DNA isolation and purification .
Gel electrophoresis \ Gel imaging . ELISA
. DNA sequencing
. PCR primer design
. Cell culture . Assay
development
Experience: Complete Genomics, Inc., Mountain View, Ca.
Molecular Biologist, Reagent Manufacturing
November 2010 - August 2011
. Formulation and QC testing (pH, conductivity) of a variety of
reagent and buffer solutions in support of high throughput sequencing
operations
. Formulation and functional testing of bulk master mixes used in
downstream enzymatic processes to generate genomic libraries. Functional
testing included routine molecular biological techniques such as PCR,
digestion/ligation rxns, DNA purification, agarose gel electrophoresis,
and gel imaging.
. Assisted in monthly laboratory physical inventory counts and used
ERP/LIMS software on a daily basis for reagent inventory and time
tracking purposes
. Trained new employees in reagent formulation, functional testing,
and data analysis.
Affymetrix, Inc., Santa Clara, Ca.
Research Assistant, Reagent Manufacturing
June 2009 - April 2010
. Testing of incoming reagent raw materials utilizing HPLC for
chemical purity and identity analysis
. Bulk formulation and functional testing of kit reagents in a cGMP
environment to support Affymetrix product line
. Preparation of process deviation and NCMR reports, protocol
and batch record change requests, and data reports related to
reagent stability studies.
Medarex, Inc., Milpitas, Ca.
Research Associate III,
Molecular Immunogenetics
April 2006 - June 2009
. Development of a quantitative based PCR genotyping assay
involving the design and validation of a comprehensive primer
set for the detection of all human transgenes in transgenic
mouse colony and adaptation of the assay to automated liquid
handling platform
. Routine genotyping and ELISA for the detection of human
transgenes and their associated antibodies for in-house transgenic
mouse colony
Affymetrix, Inc.
Production
Associate, Assay Services
June 2005 - November 2005 (South San
Francisco, Ca.)
Research Associate, Genomic Collaborations
August 2004 - December 2004
(Santa Clara, Ca.)
. Generation of high quality SNP genotyping data utilizing
Affymetrix high- density GeneChip DNA Microarrays
. Hands-on experience with Affymetrix GeneChip Scanner 3000,
Fluidics station 400/450 and associated
GeneChip Operating Software (GCOS), and geneChip DNA Analysis Software
(GDAS)
Deltagen, Inc., Redwood City, Ca.
Research II
December 1999 -
February 2004
Molecular Biology - Assay Development / Cloning
. Generation of constructs for targeted gene knockouts in mice
involving cloning, restriction enzyme
digests, and DNA purification
. Assembly and analysis of mouse genomic DNA sequences using
Sequencher software
. PCR primer design and validation to facilitate identification
of homologous recombination events within embryonic stem cells
. Developed quality control PCR assay for the detection of
Helicobacter spp. in the in-house transgenic mouse colony.
Molecular Biology - Screening / Genotyping
. High throughput
screening for homologous recombination events within embryonic
stem cell cultures using standard PCR
. High throughput genotyping of mouse tissues using both
standard and quantitative real-time PCR methods (ABI Sequence
Detection System 7900 HT)
. Utilization of basic cell culture techniques for maintenance
of embryonic stem cell cultures
. Data analysis and data entry into database
. Training new members in the group
Novartis Seeds, Inc., Gilroy, Ca.
Research Assistant I, Molecular
Markers Group
March 1999 - December 1999
. DNA sequencing associated with molecular marker primer
development using Perkin Elmer ABI 377
. High throughput genotyping for the detection of disease
resistance genes in various plant species involving PCR, agarose gel
electrophoresis, and gel imaging.
. Genomic and plasmid DNA isolation and purification
. Routine laboratory maintenance including ordering of all
supplies and reagents, buffer and media preparation, and
cleaning/autoclaving of glassware
Computer Proficient with both PC and Mac platforms, Microsoft Word,
Microsoft Applications: Excel, Microsoft Power Point, Sequencher,
Primer Express, File Maker, and BLAST search tools
References: References available upon request
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