research associate

JANKI DESAI

*** ******** **** ***# ****, Somerset, NJ 08873 Ph : 508-***-**** E-MAIL

:*****.********@*****.***

OBJECTIVE

I am seeking a position which can help me utilize as well as develop my

skills and knowledge and can help me gain experience.

Summary

Highly Motivated Master's Degree holder from NYU-Poly with over two years

experience working in a laboratory environment. Extensive knowledge and

experience in techniques such as ELISA, RT-PCR, Histology,

Immunohistochemistry Staining techniques, Mammalian Cell Culture

maintenance and expansion, Various Blotting Techniques, Assay Development,

Cell Transduction, Biomaterials, Fluoroscence Microscopy, etc. Comfortable

with working in a team or individually. Excellent interpersonal and

communication skills.

EDUCATION

MASTER OF SCIENCE IN BIOTECHNOLOGY

BACHELOR OF SCIENCE IN BIOTECHNOLOGY

Polytechnic Institute of New York University

Sardar Patel University

January 2011

June 2008

GPA: 3.9

GPA: 3.75

Relevant Professional Experience

. Research Associate at

Celgene

October 2011 -

Present

> Establish and maintain Cell culture and Cell Expansion on

Biomaterial scaffold.

> Establish and Perform Cell based and Biochemical Assays such as

simplex and multiplex ELISAs and Cell proliferation assays.

> Determination of Gene Expression Profile of Cells through RT-PCR.

> Perform histological studies; fixation, dehydration, infiltration,

embedding, sectioning, etc.

> Perform Immunohistochemistry Staining techniques to assess protein

deposition on biomaterials by cells.

> Daily Lab maintenance such as maintaining lab supplies and

inventories, cleaning equipments, writing reports and presentations,

etc.

. Volunteer Research

Assistant at the NYU

Langone Medical Center

September

2010 - September 2011

> Determination of effects of Adenoisne receptors on osteoclast

formation by using macrophage cell lines.

> Cell Culture, TRAP staining, Von Kosaa staining (bone resorption

assay), Protein Assay, protein extraction and gel electrophoresis.

> ELISA for determination of antibody's expression, SDS - PAGE and

western blotting for NFATc1 expression by adenosine - 1 receptor.

> Participate in Lab meeting and Maintain lab supply and chemicals.

Other Experience

. TA (Teaching Assistant) at NYU-POLY

September 2009 - December 2009

> Managed undergraduate students, taught them & graded them.

. GA (Graduate

Assistant) at

NYU-POLY

January

2009 - May 2009

> Performed Administrative duties for the Dean.

> Organized conferences.

. Lab Assistant at Bhailal Amin Hospital, INDIA

May 2008 - December 2008

> Performed diagnostic tests for sugar, urea, creatinine, protein,

lipids, etc on human samples from a hospital.

> Performed staining protocols, aerobic culture and blood culture of

microbes for antibiotic sensitivity test.

> Handling of different diagnostic equipments.

. Microbiology assistant at Gujarat Life Sciences

February 2007 - April 2008

> Cultured microbes for preparation of solid and liquid

inoculums for use in seed treatment and

biofertilizers.

> Maintained system parameters such as pH, temperature and degree of

aeration of fermentors and bioreactors.

> Preparation of media and buffers, handling cell cultures.

Publications

. Heat-dried human placental ECM supports human amnion derived adherent

cell (AMDAC) growth and induces secretion of growth factors and

cytokines

JJ Desai, DM Gregor, JO Ngoje, D Hou, A Kaplunovsky, R Zaka, RJ

Hariri, WT Hofgartner and MB Bhatia

Celgene Cellular Therapeutics, USA

J Tissue Eng Regen Med 2012; 6 (Suppl. 1): 170

. Bioprinting a hybrid scaffold containing human placenta derived

extracellular matrix

X Guo, DM Gregor, JJ Desai, JO Ngoje, A Kaplunovsky, R Zaka, RJ

Hariri, WT Hofgartner and MB Bhatia

Celgene Cellular Therapeutics, USA

J Tissue Eng Regen Med 2012; 6 (Suppl. 1): 376

Abilities and Key Skills

Lab Skills

. Experience with Mammalian cell and tissue culture techniques.

. Experience with Transduction of human cells.

. Immunochemical and biochemical methods including simplex and multiplex

ELISA, RT-PCR, gel electrophoresis, SDS-PAGE, southern & western

blotting, protein assay and enzyme assay and knowledge of cloning.

. Basic Animal handling

. Microbial culture handling, staining method, sterility testing.

. Confident in use of other biological instruments.

. In depth knowledge of research, laboratory maintenance (prepare

buffers, solutions, sample, reagent and materials), labeling of

vials, laboratory equipment maintenance and statistical method

. Analytical techniques: polari meter, conduct meter, PH meter,

colorimeter, photometry, spectroscopic techniques (UV, IR

spectrophotometer).

. Ability to summarize and present research data to project teams,

familiar with GMP environment, documentation and efficiently

collaborate with other team members

. Calibration of fermentors system parameters, for example; pH,

Temperature, and Degree of Aeration.

. Excellent computer skills in Microsoft word, excel, power point, Photo-

shop and Chem Draw.

. Accuracy in data collection and maintenance, good book-keeping ability

Interpersonal Skills

. Highly Self Motivated, sincere and hard-working with a strong work

ethic.

. Flexible and Friendly approach, ability to work independently and as

part of a team and Flexibility to accommodate peaks in workload.

. Proven success from university and work experience, demonstrates

skills in leadership by envisioning, direction setting and enrolling

others, has problem solving abilities, creativity & innovation. Takes

initiative & follows-through. Excellent communication skills.

Projects

. Development of the drug jk145 for the treatment of Rheumatoid

Arthritits - a virtual project

Designed a drug for the cure of Rheumatoid Arthritis, a

systemic autoimmune disease. The drug targets the Cannabinoid

receptor 2, activating it and thus reducing inflammation in joints.

. Development of a biosensor for gout - a virtual project

Designed a biosensor for the diagnosis of Gout. The

biosensor detected Uric Acid in blood by the use of immobilized

Uricase enzyme on an optic fibre.

Coursework

DNA Technology & Genetic Engineering, Environmental & Industrial

Biotechnology, Molecular Immunology & Virology, Biomolecules,

Energetics and Genetics, Biotechnology and Healthcare, Biosensors and

Biochips, Biotechnology in Pharmaceutical Industry, Materials in

Medicine, Nanocomposites, Enzyme Catalysis, Advanced Cell and

Molecular Biology, Bioanalytical Chemistry, plant tissue culture.

REFERENCE AVAILABLE ON REQUEST

Contact this candidate