Assistant University
Resume:
SILVANA CONSTANTINESCU
Phone: 310-***-**** Email: *********@*****.***; ********@***.***
Address: **** **** *******, *** *****, CA 90732
Immigration status: Permanent resident
UMMARY OF QUALIFICATIONS
S
Detail oriented and highly educated Ph.D. candidate, demonstrating extensive knowledge of biological sciences
and biochemistry. Demonstrates a highly interdisciplinary scientific background, a genuine passion for
research, and a natural ability to collaborate with team members to accomplish all objectives. Currently seeking
a position in which will effectively utilize all acquired skills, abilities, and areas of expertise as follows:
Biotechnology, Biochemistry Genetics/Molecular Biology Laboratory Techniques
& Biological Sciences Presentation Development Research & Analysis
DNA/Protein Techniques Fatty Acid Metabolism & DNA/Protein Techniques
Pharmacology Metabolic Signaling Team Collaboration
DUCATION HISTORY
E
University of Southern California Los Angeles, CA Ph.D., Dept. of Biological Sciences (2005 – Present)
Area of research: Fatty acid metabolism and metabolic signaling
Advisor: Lorraine P. Turcotte, PhD
Southern Methodist University Dallas, TX M.A., Dept. of Biological Sciences (2005)
Area of research: Genetics and Molecular Biology
Advisor: Richard S. Jones, PhD
University of Bucharest Romania M.S., Summa Cum Laude, Dept. of Biochemistry, Faculty of Biology
(2001)
Specialization: Biochemistry and Molecular Biology
Advisor: Anca Dinischiotu, PhD
Thesis: Constantinescu, S. The Enzymes involved in Oxidative Stress, Department of Biochemistry, Faculty of
Biology, University of Bucharest, Romania
University of Bucharest Romania B.S., Dept. of Biochemistry, Faculty of Biology (2000)
Major: Biochemistry
Advisor: Tatiana Vassu, PhD
ERTIFICATIONS
C
2005 Present Cardiopulmonary Resuscitation (CPR), American Red Cross
2005 Present First Aid, American Red Cross
ROFESSIONAL PROFILE
P
Graduate Research Assistant, Integrative and Evolutionary Biology Program, Department of
2010
Biological Sciences, University of Southern California, Los Angeles, CA
Collaboration in a study “Efficacy of the 5 Hour Energy Drink” conducted by Dr Girandola from
2009
Department of Kinesiology, University of Southern California, Los Angeles, CA
2006 2007 Graduate Student Representative, College Graduate Student Forum for Integrative and
Evolutionary Biology Program, Department of Biological Sciences, University of Southern
California, Los Angeles, CA
Graduate Student Representative Integrative and Evolutionary Biology Program, Department
2006 2008
of Biological Sciences, University of Southern California, Los Angeles, CA
2006 Present Graduate Teaching Assistant, Integrative and Evolutionary Biology Program, Department of
Biological Sciences, University of Southern California, Los Angeles, CA
Laboratory Courses: EXSC205L: The Science of Human Performance
2009 Summer Semester EXSC 205L: The Science of Human Performance
SILVANA CONSTANTINESCU PAGE 2
ROFESSIONAL PROFILE – CONTINUED
P
Graduate Teaching Assistant, Molecular and Computational Biology Program, Department of
2005 2006
Biological Sciences, University of Southern California, Los Angeles, CA
Laboratory Courses: BISC 120: General Biology: Cell Biology and PhysiologyBISC 220:
General Biology: Organism Biology and Evolution
Library Assistant, Southern Methodist University, Dallas, TX
2004 2005
Graduate Research Assistant, Molecular Biology and Genetics Laboratory, Department of
2003 2005
Biological Sciences, Southern Methodist University, Dallas, TX
Graduate Teaching Assistant, Department of Biological Sciences, Southern Methodist
2001 2003
University, Dallas, TX
Laboratory Courses: BIOL 1401L & 1402L: Introductory Biology
BIOL 1303L & 1304L: Essential in Biology
Research Assistant, Nicolae Paulescu Institute, Bucharest, Romania
2000 2001
ONORS & AWARDS
H
2011
1.
Integrative and Evolutionary Summer Merit Award, Department of Biological Sciences, University
1.
of Southern California, Los Angeles, CA
2. WiSE Travel Grants for Graduate Students and Post doctoral Fellows Fund, College of Letters,
Arts & Sciences, University of Southern California, Los Angeles, CA
Donegan Merit Fellowship, Department of Biological Sciences, University of Southern California,
3.
Los Angeles, CA
2.
2010
3.
IEB Interdisciplinary Research Merit Award, Department of Biological Sciences, University of
1.
Southern California, Los Angeles, CA
Conference Attendance Award, College of Letters, Arts & Sciences, University of Southern
2.
California, Los Angeles, CA
WiSE Travel Grants for Graduate Students and Post doctoral Fellows Fund, College of Letters,
3.
Arts & Sciences, University of Southern California, Los Angeles, CA
Carolyn tum Suden/Francis A. Hellebrandt Professional Opportunity Award, The American
4.
Physiological Society. Award to be presented within Experimental Biology Conference, Aneheim
The Kinesiology Outstanding Teaching Assistant Award, Department of Kinesiology, University of
5.
Southern California, Los Angeles, CA
2009
4.
Integrative and Evolutionary Biology Merit Award, Department of Biological Sciences, University
1.
of Southern California, Los Angeles, CA
2. Conference Attendance Award, College of Letters, Arts & Sciences, University of Southern
California, Los Angeles, CA
3. Johnnye Langford Merit Award, Department of Kinesiology, University of Southern California,
Los Angeles, CA
5. 2008 & 2007
1. Interdisciplinary Graduate Research Grant, Integrative and Evolutionary Biology Program,
Department of Biological Sciences, University of Southern California, Los Angeles, CA
6. 2000 01
1. Scholar of the Year, Department of Biochemistry, Faculty of Biology, University of Bucharest,
Romania
2000 01
o MS Scholarship, Biochemistry, Department of Biochemistry, Faculty of Biology, University of
Bucharest, Bucharest, Romania
1998 2000
o BS Scholarship, Biochemistry, Department of Biochemistry, Faculty of Biology, University of
Bucharest, Bucharest, Romania
RESENTATIONS
P
Constantinescu, S., Turcotte, L.P. The effect of low expression of Receptor Interacting Protein 140
7.
(RIP140) in normal and L6 cells treated with high fatty acid. Experimental Biology, Washington, DC, April
2011. Poster Presentation
8. Constantinescu, S., Turcotte, L.P. Insulin signaling is affected by a low Receptor Interacting Protein 140
(Rip140) expression in L6 skeletal muscle cells. Presented at GPSS Poster Symposium, University of
Southern California, Los Angeles, CA, April 2011. Poster Presentation
Constantinescu, S., Turcotte, L.P. Receptor Interacting Protein 140 (RIP140) silencing via RNAi interferes
9.
with fatty acid metabolism but not with glucose uptake in L6 muscle cells. RNAi &miRNA World
Congress, Boston, MA, May 2010. Poster Presentation
Constantinescu, S., Turcotte, L.P., Low RIP140 Expression Partially Restores Metabolic Flexibility in L6
10.
Muscle Cells Treated With High Fatty Acid. Experimental Biology, Anaheim, CA, April 2010. Poster
Presentation
Constantinescu, S., Turcotte, L.P. Suppression of Receptor Interacting Protein 140 (RIP140) expression in
11.
L6 muscle cells impairs the regulation of insulin. Presented at Graduate and Professional Appreciation
Week Research Fair, University of Southern California, Los Angeles, CA, November 009. Poster
Presentation
Constantinescu, S., Turcotte, L.P. Suppression of Receptor Interacting Protein 140 (RIP140) expression in
12.
L6 muscle cells impairs the regulation of insulin. ACSM. Southwest Chapter 29th Annual Meeting, San
Diego, CA, October 2009. Poster Presentation
Constantinescu, S. Fatty acid metabolism and glucose uptake are differently affected by aspirin treatment
13.
in muscle cells exposed to high saturated fatty acid availability. Presented at Graduate and Professional
Appreciation Week Research Fair, University of Southern California, Los Angeles, CA, March 2009
Constantinescu, S. Insulin Effects on LCFA Metabolism: PDK1, Akt1, Akt2 and aPKC ζ Involvement.
14.
Presented at University Research Day, University of Southern California, Los Angeles, CA, 2007
Wang, L., Constantinescu, S., Zhang, J., Benes, J., Jones, R. Distributions of Polycomb group protein
15.
complexes at a target gene and their effects on chromatin structure. Presented at 46th Annual Drosophila
Research Conference, San Diego, CA, 2005
Constantinescu, S. Mes 4 maternal effect sterile from Drosophila melanogaster. Presented at University
16.
Research Day, Southern Methodist University, Dallas, TX, 2004
Constantinescu, S. Polycomb like a PcG protein from Drosophila melanogaster. Presented at University
17.
Research Day, Southern Methodist University, Dallas, TX, 2003
UBLICATIONS
P
Constantinescu, S., Turcotte,L.P. (2011) Low Receptor Interacting Protein 140 (RIP140) Expression
1.
Adversely Impacts Proximal Insulin Signaling in L6 Skeletal Muscle Cells. FASEB J (25), March 17:
lb584 (Meeting Abstract)
Constantinescu, S., Turcotte, L.P., (2010) Low RIP140 Expression Partially Restores Metabolic
2.
Flexibility in L6 Muscle Cells Treated With High Fatty Acid. Faseb J (24): 1045.3 (Meeting Abstract)
ROFESSIONAL MEETING PARTICIPATION
P
May 2010 RNAi &miRNA World Congress, Boston, MA: Poster Presentation
Apr. 2010 Experimental Biology, Aneheim, CA: Poster Presentation
Oct. 2009 Southwest Chapter of the American College of Sports Medicine (regional meeting), San Diego,
CA: Poster Presentation
Nov. 2007 Southwest Chapter, American College of Sports Medicine, San Diego, CA: Attendance
NVITED PRESENTATIONS
I
Suppression of Receptor Interacting Protein 140 (RIP140) expression in L6 muscle cells impairs the
regulation of insulin. Departmental Seminar, Integrative and Evolutionary Biology Program, Dept. of
Biological Sciences, University of Southern California, December 2009
ABORATORY TECHNIQUES & TECHNICAL SKILLS
L
General Techniques: Physiology, Fat Metabolism, Genetic manipulations, chromosome mapping, phenotype
characterization, genetic markers, lifespan studies in D.melanogaster
DNA Techniques: DNA Extraction, RNA extraction, DNA electrophoresis, PCR, nested PCR, RT PCR,
DNA sequence analysis, Quaiagen purification kit for DNA purification, DNA analysis
using spectrophotometer. RNAi interference performed in S2 Drosophila cells and L6
skeletal muscle cells
Protein Techniques: Western Blotting, cloning, bacteria manipulations, SDS PAGE, Chromatin
immunoprecipitation (ChIP), BCA. Extensive experience in tissue and culture cells S2
Schneider cells and skeletal muscle cells, fatty acid uptake and fatty acid oxidation
measurements using scintillation counter, microbiology techniques (E.coli). Primers
design for PCR, RT PCR, DNA sequence analysis, protein sequence analysis using
NBCI, BLAST, GenBank and sequence alignments (CLUSTALW)
Operating Systems: MacOS10, PC, MS (Excel, PowerPoint, Word), Windows XP, Windows 98/2000NT
Software: DNA strider, RasMol, Adobe Illustrator/Photoshop, Statsview, ScionCorp and
ImageJ(protein gels analysis software)
EMBERSHIPS
M
2009 Present American College of Sports Medicine (ACSM)
2006 Present American Physiological Society (APS)
2004 Present European Federation of Biotechnology
References:
1. Lorraine P Turcotte, PhD ********@***.***; Phone: 213-***-****
2. Jill McNitt Gray, PhD ******@***.***; Phone 213-***-****
3. Casey Donovan, PhD *******@***.***; Phone 213-***-****
Contact this candidate