Project Assistant

Lei Xu, MS.

***** ********** ***, #****

Cupertino, CA 95014

C: 650-***-****

E-mail: ****@*******.***

Objectives:

To join a dynamic team in development and implementation of novel

technologies in biomedical sciences.

Technical Skills:

1.Working experience at Amgen as a research associate

2. Two year Drug discovery research experience.

In Cell Western: Phospho ACC on HepG2 and H1299 cell lines; Membrane

immobilized ligand binding assay (plate assay) of adiponectin; Cell

membrane preparation; western blot analysis. PRK plate assay;

3. Proficient in major molecular biology techniques including:

Antibody Expression. DNA isolation and purification. RNA isolation and

purification. Protein isolation. Southern dot blot. Northern blot. Western

blot analysis. Molecular cloning. UV/VIS spectrophotometry. In vitro

transcription. Immunofluorescence staining. DNA sequencing. DNA fragment

sequencing with genome walker kit. RNase protection assays. Restriction

enzyme analysis. Immunopanning. PCR. RT-PCR. TaqMan. Primer and probe

designing. ELISA. Sequencher analysis. Up-Scaling Protein expression with

Roller Bottles.

4.. Extensive experience in major cell biology techniques including:

Established and maintained stable cell lines and human primary cell lines

Primary and secondary tissue Culture (Cell culture). In vitro cell-based

assays. Mammalian cell transfection (electroporation and lipofection).

5. In vivo animal models including:

Small animal handing and Stereotaxic Intracerebral Injection. Analyzing

DNA and RNA from animal tissues.

6. Proficient in searching biochemical information from internet and using

computer programs including Word, PowerPoint, Adobe Photoshop, and Exel.

7. A self motivated, hardworking, and team player. Good communication and

problem solving skills and data analysis. Effective multi-tasking skills,

strong organizational, and record keeping.

Research Experience:

1. 01/2009-04/2010

Research consultant

Worked on drug development project.

2. 4/2007-10/2007

Research associate

Amgen Inc.

Participated in Antibody engineering project (Adiponectin protein

engineering); Involved in cloning service. Transfection; Up-Scaling Protein

expression with Roller Bottles. Western blot.

3. 2/2005-2/2007

Research Associate

Rigel Pharmaceuticals, Inc.

Participated in Drug discovery Project (Diabetes disease type 2 with

Adiponectin)

Developed cell based assays (In Cell Western: Phospho ACC on HepG2 and

H1299 cell lines) Enzyme kinetics (PRK plate assay), Assay development and

automated liquid handling; Membrane immobilized ligand binding assay (plate

assay) of adiponectin; Cell membrane preparation; western blot analysis.

PRK plate assay. Experience with cell based assay readouts of receptor

ligand interactions.

4. 5/2004-10/2004

Research Associate (Temp)

Cell Genesys Inc.

Worked on Anti-Angiogenesis projects.

Constructed many antibody expression vectors.

Cell based assays. Cultured CHO cell line, 293 cell line and NSO cell line.

Transient transfection. Antibody Expression. ELISA.

5. 6/2001-4/2003

Research Associate

Sangamo Biosciences, Inc.

Participated in the gene therapy projects in the cardiovascular therapeutic

team (Angiogenesis).

Analyzed many gene expression levels such as mouse, rat and human VEGF mRNA

with real time quantitative RT-PCR (Taqman).

Designed many Taqman primer and probe sets including various VEGF isoforms.

Performed ELISA and Western blot analysis to quantitate and detect VEGF

protein expression.

Cultured many different types of cells including primary cell culture and

induced differentiated skeletal muscle cells. Lipo-transfected SKMC and 293

cells with plasmid DNA (in vitro cell-based assays).

Made and validated the VEGF in situ DIG-labeled RNA probes to detect VEGF

expression level in mouse tissues with In vitro transcription kit.

Sequenced many DNA fragments, such as pig FGF5, Pig VEGF and Rabbit VEGF

promoter regions using the Genome Walker kit.

6. 7/1999-6/2001

Researcher

Dept. of Neurosurgery, Stanford University

Worked on the gene therapy project for brain tumors (Oncology).

Worked on neuronal transplantation project for Parkinson's disease.

Used semi-quantitative RT-PCR to quantitate the expression level of

angiopoietin factors such as ang-1, ang-2, VEGF, and Tie-2 in the glioma

rat model.

Verified that sonic hedgehog promotes early postnatal sympathetic cell

proliferation and promotes tyrosine hydroxylate induction of sympathetic

cells in vitro using immunofluorescence staining

7. 1/1996-5/1999

Master of Science and Research Assistant

Dept. of Biology, University of South Alabama

Gained considerable expertise in cellular and molecular biology approaches.

Constructed an antisense Beta globin cDNA gene expression vector.

Established and maintained stable cell lines and human primary cell lines.

Transfected antisense Beta globin cDNA expression vector into mouse

erythroleukemia cells and human primary erythroid progenitors by

electroporation.

Analyzed RNA expression level by RNA protection assay.

Education:

1. 1/1996-5/1999

Dept. of Biology, University of South Alabama

Master of Science

2. 9/1981-6/1987

Shanghai Medical University, Shanghai, P.R.China

Doctor of Medicine

Publications and Abstracts:

Rebar EJ, Huang Y, Hickey R, Nath AK, Meoli D, Nath S, Chen B, Xu L, Liang

Y, Jamieson AC, Zhang L, Spratt SK, Case CC, Wolffe A, Giordano FJ.

Induction of angiogenesis in a mouse model using engineered transcription

factors. Nature Medicine. 2002. 8(12): 1427-32.

Williams, Z., Tse, V., Hou, L., Xu, L., and Silverberg, G., Sonic hedgehog

promotes proliferation and tyrosine hydroxylase induction of postnatal

sympathetic cells in vitro. NeuroReport. 2000. 11(15):3315-3319.

Xu, L., A.E.Terry, C. Monteiro, and B.S.Pace. Enhanced Gamma globin

biosynthesis by antisense beta globin RNA transcripts. Gene Therapy. 2000.

7:438-444.

Xu, L., and B.S.Pace. 1998. Modulated human globin gene expression: Role of

antisense expression vectors. Cell.Mol.bio.Letters. 1998. 3:423-433.

Edward JR., L. Zhang, Y. Lee, Y. Liang, L. Hinh, B. Johnstone, L. Xu, CL.

Dent, P. Liu, X. Li, TD. Schaal, EJ. Wolffe, SK. Spratt, J. Rokovich**, CC.

Case, CO. Pabo. Robust Activation of Vascular Endothelial Growth Factor-A

Using Designed Zinc Finger Protein Transcription Factors. The 6th American

society of gene therapy, May, 2003, Washington, DC.

Xu, L., C. Monteiro, and B.S.Pace. Suppression of human Beta globin chain

synthesis mediated by a mammalian antisense expression vector. Presented at

the American society of gene therapy, May, 1998, Seattle, WA.

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