Assistant Engineering

Rohan Nandkumar

Actively seeking employment in a biotech/pharmaceutical organization, with focus on protein

engineering

*****.******@*****.***

Summary

My educational qualifications and my 1.5 years of experience as a Research Assistant in Dept. of Cell

Physiology in TTUHSC, Lubbock, TX, both have helped me develop significant laboratory skills. My work has

primarily been protein engineering and I'm technically proficient in techniques pertaining to protein expression,

purification, structure/function relation and protein crystallography and mutational analysis. My skill set such as

scientific thinking capability, troubleshooting, method improvisation, scientific writing and documentation, and

my preparedness to work as a responsible individual as well in a team, is clearly evident from my Thesis project

work. I'm flexible towards exploring all other avenues of my discipline, and would be willing to work for an

organization that advocates research in the biotechnology/pharmaceutical discipline. My career objective is to

attain a professional expertise in the field of biotechnology through continuous learning, research and applied

knowledge, with an aptitude to contribute to the society.

Education

Texas Tech University

Master of Science (M.S.), Biotechnology, 2011 - 2013

Grade: GPA 3.93/4.00

Shivaji University

Bachelor of Engineering (B.E.), Biotechnology, 2005 - 2010

Grade: 3.18/4.00

Courses

Master of Science (M.S.), Biotechnology

Texas Tech University

Methods in biotechnology

Genes

Applied Multivariate Statistics

Analytical Separation Science

Biomedical Informatics

Scientific Communication

Responsible Course of Conduct

Principals and Applications of DNA technology

Deep Saturation Mutagenesis/X-ray Crystallography

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Experience

Research Assistant at Texas Tech University Health Sciences Center

April 2012 - August 2013 (1 year 5 months)

CLONING AND SUBCLONING:

Site-directed mutagenesis/primer design

Polymerase Chain Reaction (PCR)

Plasmid DNA isolation

Bacterial transformation

PROTEIN BIOCHEMICAL ANALYSIS:

Fast Performance Liquid Chromatography (FPLC)

Affinity column chromatography

Sodium Dodecyl Sulphate Polyacrylamide

Gel Electrophoresis (SDS-PAGE)

GE-PhastSystem Separation and Development unit

PROTEIN STRUCTURE AND FUNCTION ANALYSIS

Circular Dichroism (CD)

Isothermal Titration Calorimetry (ITC)

Fourier Transform Infrared Calorimetry (FTIR)

Hanging drop technique for crystal tray setup

MOSQUITO robot operation for protein crystallization

PYMOL software

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Projects

Biophysical Characterization of Mitsugumin 53 Protein

August 2012 to August 2013

Members:Rohan Nandkumar

THESIS

Involved the in vitro analysis of the MG53 protein using various techniques that are capable to determine the

protein's interaction with its potential ligands. MG53 is a muscle specific protein with membrane repair

mechanistic properties, and implicates to be a potential therapeutic in the treatment of muscular dystrophy

and related diseases

*Literature Review

*Experiment Design

*Protocol Structuring

*Data Interpretation and Improvisation

*Troubleshooting

*Scientific Documentation

Skills & Expertise

SDS-PAGE

Gel Electrophoresis

Cell Culture

Protein Expression

Agarose Gel Electrophoresis

DNA extraction

Protein Purification

Molecular Cloning

Plasmid Isolation

Affinity Chromatography

FPLC

Protein Structure

Protein Structure Prediction

Circular Dichroism

Isothermal Titration Calorimetry

Protein Crystallization

Pymol

FTIR

Lyophilization

Extrusion

PCR

Mutagenesis

Western Blotting

Primer Design

Bacterial Transformation

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Publications

STUDYING IN-VITRO APPLICABILITY OF HOSTINDEPENDENT STRAIN OF BDELLOVIBRIO

BACTERIOVORUS AS A BIOFILM REDUCING AGENT

International Journal of Current Research and Review November 2011

Authors: Rohan Nandkumar, Manjiri Jadhav, Anjali Pukar

ABSTRACT:

Biofilms are surface-attached microbial communities with phenotypic and biochemical

properties distinct from free swimming planktonic cells. The capability of the gram-negative

predatory bacterium Bdellovibrio bacteriovorus to control and reduce an existing Serratia

marcescens biofilm was evaluated by colony biofilm assay. A reduction in biofilm biomass was

observed as early as 2 h after exposure to the predator, and as much as 75% reduction after 6hrs

of exposure. The ability of B. bacteriovorus to reduce an existing biofilm was confirmed by

reduction in CFUs after exposure of predator.

Languages

English

Hindi

Marathi

Malayalam

Interests

Digital Photography, Drawing, Music

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Rohan Nandkumar

Actively seeking employment in a biotech/pharmaceutical organization, with focus on protein

engineering

*****.******@*****.***

Contact Rohan on LinkedIn

Page5

Contact this candidate