Microbiology skills: aseptic and sterile techniques, clinical specimen

Santosh Rajbahak

*** ******** ***

Malden, MA ***48

Phone: 857-***-****

Email: *.*********@*****.***

OBJECTIVES

Dedicated to microbiology and immunology with excellent technical, analytical and

communication skills demonstrated by three years of experience in a pathology laboratory.

Exploring career opportunities in microbiology and immunology. My long-term goal is to

obtain a doctorate degree in molecular biology or immunology, leading to a research career in

the health sciences.

EDUCATION

YEAR INSTITUTION MAJOR DEGREE

2008-2012 Tribhuvan University Medical Microbiology Master

2005-2008 Tribhuvan University Microbiology Bachelor

2003-2005 Higher Secondary Education Science High School

Board

WORK EXPERIENCE

National Health Care and Support Foundation

April 2012- January 2013

Research Microbiologist

Assist in design and management of research projects in the area of HIV/AIDS and

tuberculosis. Conduct and document research experiments under the guidance of research

supervisor. Responsible for preparing samples and related test media and reagents as per the

research demand. Assist in analysis and assessment of the resulting data. Prepare periodic

report and submit to the assigned senior research officer. Follow-up, monitor and report

activities on HIV/AIDS and Tuberculosis and ensure that these are in succession to the

objective of the project. Organize workshop and training and assist to implement the planned

activities. Participate in related trainings and workshops.

Everest International Clinic and Research Center (EICRC)

April 2009- March 2012

Microbiologist

Conduct bacteriological, parasitological, virological and serological examination of human

clinical and environmental samples. Perform microscopy, culture, antibiotic sensitivity test

and molecular techniques like PCR, electrophoresis, ELISA, cell based assays and other

serological diagnostic tests. Prepare and inspect quality of different laboratory media and

reagents. Development of the laboratory methodologies, implementation and maintenance of

the cGMP. Assist to design, conduct and document different research experiments under the

guidance of the researcher supervisor. Maintain records and prepare reports related to the

work in compliance with EICRC policies and procedures. Participate in meetings, seminars

and workshops related to microbiology as well as health issues and implement information

accordingly. Guide students and laboratory staffs on procedures and techniques.

SKILLS

Microbiology skills: aseptic and sterile techniques, clinical specimens collection and

processing, microscopy and staining, laboratory investigations for diagnosis of infectious

diseases caused by bacteria, fungi, viruses and parasites, perform and interpret immunological

and serological tests. Bacterial staining, plating methods (streak, spread, pour, replica),

enumeration and identification of bacteria, use of biological safety cabinets, media and buffer

preparation, water sample analysis by membrane filtration and MPN, food microbiology,

diagnostic biochemical tests for enteric bacteria, use of selective media, quality control

testing, GMP operations, sterility testing and use of the laboratory equipments.

Implementation and maintainance of cGMP.

Biochemistry and molecular biology skills: DNA extraction, plasmid DNA preparation,

restriction enzyme digestion, PCR, ELISA, cell based assays, agarose gel electrophoresis,

SDS-PAGE, cell fractionation by differential centrifugation, preparation of cells for

cryopreservation, use of hemocytometer.

Chemistry skills: solution preparation, titrations, extractions filtrations, separations, simple

and fractional distillation, solubility tests, determination of index of refraction, freezing point

depression, thin- layer chromatography, liquid chromatography, reflux, recrystallization.

Computer skills: Microsoft office (Word, Excel, Power point), photoshop, typing (50

words/minute), PubMed, SPSS.

Language skills: English, Nepali, Newari, Hindi.

OTHER COURSES AND WORKSHOPS

• Training on molecular and immunological techniques organized by Intrepid

Nepal/ Center for Molecular Dynamics, Thapathali, Kathmandu, Nepal (17 th-

19th October, 2012).

• Training workshop on health research systems, research proposal

development, from Nepal Health Research Council, Kathmandu Nepal, (30th

Sep- 05th Oct 2012).

• Forty-five days course on ‘Quality assurance and in plant training’ from

himalayan spring water, Rasuwa, Dhunche (1st July–15thAugust, 2011).

• Seven days governance accountability training course from Action Aid

International Nepal (9th – 15th November, 2011).

• Seven days workshop on research skill development, proposal, thesis and

paper writing organized Tri-chandra College, Kathmandu (7th-14th September

2010).

• Statistical package for social science (SPSS) training organized by Tri-chandra

College (5th – 7th December 2009).

• Bioinformatics and its basic scope in different fields and applications in

microbiology and genetics, organized by central department of

microbiology, Tribhuvan University (14th – 18th September, 2008).

RESEARCH ACTIVITIES

Rajbahak S, Shrestha C, Singh A and Shrestha J (Submitted) . Time related changes in

bacteriological profile of burn wound infections and their antibiogram. Scientific World.

Shrestha C, Rajbahak S, Singh A and Shrestha J (Submitted) . Antibiogram and β-

lactamase production test of multidrug resistant Staphylococcus Spp. isolated from

different clinical specimens. Scientific World.

REFERENCES

Available on request

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