Plant Assistant

DHRITIMAN GHOSH

Biosystems Engineering and Soil Science

The University of Tennessee

Knoxville, TN 37996, USA

Phone: 865-***-**** (LAB)

865-***-**** (Off)

865-***-**** (mobile)

Email: *******@*****.***

EDUCATION

PhD, Microbiology, Department of Life Sciences and Biotechnology, Jadavpur University,

Calcutta, India, 2003.

Master of Science, Botany (specialization in Plant Biochemistry and Molecular Biology), Visva

Bharati University, Santiniketan, India, 1997

Bachelor of Science, Botany, Calcutta University, Calcutta, India, 1995

EMPLOYMENT

Research Assistant Professor, Biosystems Eng & Soil Sci, University of Tennessee 2010 - Present

Research Associate II, Dept of Biosystems Eng & Soil Sci, University of Tennessee 2009 - 2010

Post-doc Research Associate, Dept of Biosys Eng & Soil Sci, University of Tennessee 2005 - 2009

Post-doc Research Associate, Dept of Civil & Env. Eng., Univ. of Wisconsin-Madison 2004 – 2005

Postdoctoral Research Associate, Dept of Chemistry & Agronomy, Univ. of Kentucky 2003 - 2004

Graduate Research Assistant, Dept Of Life Sc & Biotechnology, Jadavpur University, India 1998 - 2003

GRANT FUNDING

Received ($35,000.00 Total since 2010):

Radosevich, M., D. Ghosh, N. Stewart. Development of disease resistant plants through

incorporation of bacteriophage endolysins. UTIA Ag Research Innovation Grants Program,

University Of Tenn.

Ghosh, D., G. Alexandre. Type VI secretion systems as modulators of plant-microbe associations.

M-CERV, Office of Research, University of Tennessee

Funding Requested/Pending ($989,400.00 Total since 2013):

Ghosh, D.(lead PI), M. Radosevich and D. A. Golden. Influence of bacterial communication

(quorum sensing) on shiga toxin gene expression and transfer to non-pathogenic E. coli in various

foods. Submitted to Food Safety, Nutrition, and Health: Physical and Molecular Mechanisms of

Food Contamination program of USDA-AFRI, 2013.

Radosevich, M., D. Ghosh, and G. Alexandre. Quorum-sensing mediated prophage induction in

Sinorhizobium meliloti: signaling mechanisms, impact on nodulation, and extent in other

Rhizobacteria. Submitted to Plant health and production and plant products program of

USDA-AFRI, 2013.

REFEREED PUBLICATIONS

• Srinivasiah, S., J. Lovett, D. Ghosh, K. Roy, M. Radosevich and K. E. Wommack. 2013. Dynamics

of autochthonous soil viral communities parallels dynamics of host communities under nutrient

stimulation. Appl. Environ. Microbiol. (in review)

• Srinivasiah, S., J. Lovett, S.W. Polson, D. Ghosh, K. Roy, J. Fuhrmann, M. Radosevich, and K.E.

Wommack. 2013. Direct assessment of viral diversity in soils using RAPD-PCR. Appl. Environ.

Microbiol. (in press). Published online on 21 June 2013 (doi:10.1128/AEM.00268-13)

• Prado, M. E., D. Ghosh, and S. A. Ripp. 2012. Applications of bacteriophages for the control of

veterinary pathogens, In Boulanger A, Blanc M (ed.), Bacterial Pathogens: Virulence Mechanisms,

Diagnosis and Management, Ed. NOVA Publishers, Inc., Hauppauge, NY.

• Omotayo, A.E., M. O. Ilori, O. O. Amund, D. Ghosh, K. Roy, and M. Radosevich. 2011.

Establishment and characterization of atrazine degrading cultures from Nigerian agricultural soil

using traditional and Bio-Sep bead enrichment techniques. Appl. Soil Ecol. 48 (1): 63–70

• Ghosh, D, K. Roy, K. E. Williamson, K. Eric Wommack and Mark Radosevich. 2009. Acyl-

homoserine lactones can induce viral production in lysogenic bacteria: an alternative paradigm for

prophage induction. Appl. Environ. Microbiol. 75 (22):7142-52.

• Ghosh, D, K. Roy, V. Srinivasan, T. Mueller, O. H. Tuovinen, K. Sublette, A. Peacock, M.

Radosevich. 2009. In-situ enrichment and analysis of atrazine-degrading microbial communities

using atrazine-containing porous beads. Soil Biol. Biochem. 41:1331-1334

• Ghosh, D., K. Roy, K. E. Williamson, D. C. White, K. E. Wommack, K. L. Sublette, and M.

Radosevich. 2008. Prevalence of Lysogeny among Soil Bacteria and Presence of 16S rRNA and

trzN Genes in Viral-Community DNA. Appl. Environ. Microbiol. 74: 495-502.

• Bench, S. R., T. E. Hanson, K. E. Williamson, D. Ghosh, M. Radosevich, K. Wang, and K. E.

Wommack. 2007. Metagenomic Characterization of Chesapeake Bay Virioplankton. Appl.

Environ. Microbiol. 73: 7629-7641.

• Xu, S, E. D'Angelo, D. Ghosh, J. Feliciano, S. Deo and S. Daunert. 2005. Detection of

polychlorinated biphenyls employing chemical dechlorination followed by biphenyl whole cell

sensing system.

Toxicol. Environ. Chem., 2005, 87, 3, 287-298

• Ghosh, D, B Bal, V. K. Kashyap, and S. Pal. 2003. Molecular Phylogenetic Exploration of Bacterial

Diversity in a Bakreshwar (India) Hot Spring and Culture of Shewanella-Related Thermophiles.

Appl. Environ. Microbiol. 69: 4332-4336.

• Saha, M, D. Ghosh Jr., D. Ghosh, D. Garai, P. Jaisankar, K. K. Sarkar, P. K. Dutta, S. Das, T. Jha

and J. Mukherjee. Studies on the production and purification of an antimicrobial compound and

taxonomy of the producer isolated from the marine environment of the Sundarbans. Appl.

Microbiol. Biotechnol., 2005. 66 (5), 497-505

NEWS PUBLICATION

“Viruses Play Big Role in Gene Transfer and Ecology of Soil Bacteria” in “Microbe” [the

American Society for Microbiology (ASM) magazine]. March, 2008 issue, vol.3 pp.138

PATENT / DISCLOSURE

• A novel, broad-spectrum endolysin for the control of plant pathogens (submitted for internal review).

http://utrf.tennessee.edu/abstracts/12049.pdf

• Use of a novel membrane protease from bacteriophage against gram negative pathogens (in

preparation)

SOME SELECTED PAPERS PUBLISHED IN PROCEEDINGS/MEETINGS/CONFERENCES

• D. Ghosh, K. Roy and M. Radosevich. Quorum-sensing Compound Produced by Pseudomonas

aeruginosa PAO-1 Induces Lambdaimm434 Prophage in Co-culture. American Society for

Microbiology General meeting. 2009, May 17-21, Philadelphia, PA, USA

• K. Sides, V. Srinivasan, D. Ghosh, K. Roy, K.E. Wommack and Mark Radosevich. Prevalence of

Lysogeny in Forested and Agricultural Soil Bacteria as a Function of Host Colony Formation Rate.

American Society for Microbiology General meeting. 2009, May 17-21, Philadelphia, PA, USA

• D. Ghosh, K. Roy, E. Martin, K. Williamson, K.E. Wommack and M. Radosevich. In-situ

enrichment of atrazine degrading microbial community with porous Bio-Sep beads. American

Society for Microbiology General meeting. 2008, May 5-9, Boston, MA, USA

• K. Sides, V. Srinivasan, D. Ghosh, K. Roy, K.E. Wommack and Mark Radosevich. Cultivation of

novel hosts for isolating novel bacteriophages from soil. American Society for Microbiology

General meeting. 2008, May 5-9, Boston, MA, USA

• Shifen Xu, Dhritiman Ghosh, Jessika Feliciano, Sapna Deo, Elisa D’Angelo1, Sylvia Daunert:

DEVELOPMENT OF A WHOLE-CELL SENSING SYSTEM

FOR PCBs DETECTION: Superfund Basic Research Program 2003 Annual Meeting, National

Institutes of Environmental Health, November 09-12, 2003,

Dartmouth College, Hanover, NH, USA.

(http://www.dartmouth.edu/~toxmetal/SBRP/absub.shtml)

• D. Ghosh, K. Roy, E. Martin, K. Williamson, K.E. Wommack and M. Radosevich. DIRECT

EVIDENCE FOR HORIZONTAL TRANSFER OF 16S RRNA GENE VIA TRANSDUCTION.

11th International Symposium on Microbial Ecology, Vienna, Austria, August 20 – 25, 2006.

• K. Roy, D. Ghosh, E. Martin, K. Williamson, K.E. Wommack and M. Radosevich. Soil Bacterial

communities sampeled with Bio-Sep beads exhibit a high frequency of lysogeny. 11th International

Symposium on Microbial Ecology, Vienna, Austria, August 20 – 25, 2006.

• Martin, E, Radosevich, M, Ghosh, D, Biggerstaff, J, Peacock, A, White, DC. 2005, In situ

enrichment and cultivation of phylogenetically novel atrazine degrading bacteria. American Society

for Microbiology General meeting. July 5-9, Atlanta, GA, USA

• D. Ghosh, K. Roy, E. Martin, and M. Radosevich, K. Williamson and K.E. Wommack. 2006.

Lysogeny and the horizontal Transfer of bacterial genes in Soil Viral Communities. American

Society for Microbiology General meeting. May 5 -12, Orlando, FL, USA.

• E. Martin, D. Ghosh, K. Roy, A. Peacock, D.C. White, and M. Radosevich. 2006. Cultivation of

Phylogenetically Diverse and Metabolically Novel Atrazine Degrading Soil Bacteria. American

Society for Microbiology General meeting. May 5-12, Orlando, FL, USA.

• E. Martin, D. Ghosh, K. Roy, A. Peacock, D.C. White, and M. Radosevich. In Situ Enrichment with

BioSep Beads Yields Diverse and Metabolically Novel Atrazine Degrading Soil Bacteria. The ASA-

CSSA-SSSA International Annual Meetings (November 12-16, 2006), Indianapolis, IN, USA

• Pal S and D.Ghosh : Culture Independent and culture dependent studies on bacterial diversity

present in Bakreswar hot spring (INDIA) : International Conference

on thermophiles,“Thermophiles 2001”, New Delhi, INDIA, December 2001

• Pal S and D.Ghosh : Bacterial diversity in Bakreswar hot spring . 1st Conference of the

Biotechnology Society Of India at New Delhi, INDIA, June 2001.

POST DOCTORAL RESEARCH EXPERIENCES

Discovery of the missing link between quorum sensing and bacteriophage induction (from 2007 –

2009): Bacteriophages typically are induced to a lytic cycle under stressful environmental conditions or

when the host's survival is threatened. However, stress-independent, spontaneous induction also occurs

in nature and may be cell density dependent, but the in vivo signal(s) that might trigger induction was

unknown. In this research, we report that acyl-homoserine lactones (AHLs), the essential signaling

molecules of quorum sensing in many gram-negative bacteria, can trigger bacteriophage production in

soil and groundwater bacteria. This phenomenon also was operative in a lambda lysogen of Escherichia

coli. In model coculture systems, we monitored the real-time AHL production from Pseudomonas

aeruginosa PAO1 using an AHL bioluminescent sensor and demonstrated that lambda-prophage

induction in E. coli was correlated with AHL production. As a working model in E. coli, we showed

that the induction responses of lambda with AHL remained unaffected when recA was deleted,

suggesting that this mechanism does not involve an SOS response. In the same lambda lysogen we also

demonstrated that sdiA, the AHL receptor, and rcsA, a positive transcriptional regulator of

exopolysaccharide synthesis, are involved in the AHL-mediated induction process. These findings

relate viral reproduction to chemical signals associated with high host cell abundance, suggesting an

alternative paradigm for prophage induction. (Published in Appl. Environ. Microbiol. 2009. 75

(22):7142-52).

Influence of bacterial communication (quorum sensing) on shiga toxin gene expression and

transfer to non-pathogenic E. coli (from 2011 – present): Stx, the shiga toxin-producing gene is part of

a temperate bacteriophage genome that replicates with E. coli 0157:117, known as shiga toxin-

producing E. coli (STEC) strains. These bacteriophages serve as efficient vectors for the transfer of stx

and play an important role in the evolution of new pathogens. Stx viruses can be induced from the

lysogenic strain that can infect other closely related bacteria and transduce the stx gene. But an in vivo

signal that might trigger the induction of this phage and consequently the stx gene is unknown.

Although STEC strains are foodborne pathogens, it is not known to what extent potential transduction

of stx to other bacteria could occur in food. Recently, we have discovered that homoserine lactones

(HSL), the quorum-sensing signals (QS) in Gram-negative bacteria, can induce temperate phage from

bacteria of diverse environments (Ghosh et. al. 2009) (see peer reviewed publication). In in vitro model

experiments, we have demonstrated that HSL produced by Pseudomonas aeruginosa can induce phage

production from a lambda lysogen of E. coli (Ghosh et. al. 2009). Indeed, as preliminary data I have

also observed that STEC strains can be induced by quorum sensing compounds. On the basis of this

observation and primary hypothesis I submitted a full proposal to food safety program of USDA-

AFRI which is currently under review. The main objectives of this proposal are as follws,

i) Evaluate the impact of various AHLs and autoinducer II (AI-2) on the induction of Stx-phage from

various STEC strains in vitro and in food by adding various bacteria that produce different AHLs

and AI-2 in vivo. The impact of AI-2 produced by STEC on Stx-phage induction will be evaluated

in a luxS- background strain.

ii) Determine the short and long term in situ transduction efficiencies of stx phage in response to

quorum sensing in various foods.

iii) Elucidate the molecular mechanism of AHL and AI2 mediated induction of stx phage. The genes

that directly regulate induction of Stx phage will be identified.

A novel, broad spectrum bacteriophage endolysin against various plant pathogens (from 2011 –

Present): The need for alternative antimicrobials has reawakened interest in the use of bacteriophages

(phages) to kill pathogenic bacteria. Due to some advantages, cell wall hydrolyzing enzyme(s)

(endolysins) may also be used as alternatives to infective phages and many endolysins have shown

potential as therapeutics for bacterial pathogens in animals including humans. However, endolysins of

plant pathogens have received less attention. We have discovered a lytic bacteriophage for a bacterium

isolated from an agricultural (corn field) soil closely related to Leifsonia xyli subsp. xyli (Lxx). Lxx is a

Gram-positive, coryneform bacterium that causes ratoon stunting disease (RTD) in sugarcane.

Economically, RTD is significant causing about 30% yield loss of sugarcane globally, annually and

there are no biological controls available. Our preliminary data shows that the phage as well as the

encoded endolysin has a complete lytic activity on Lxx. The encoded endolysin from this bacteriophage

also shows lytic activity on other gram positive plant pathogens such as Clavibacter michiganensis

subsp. michiganensis (a tomato pathogen) and Leifsonia xyli subsp. cynodontis (a barmuda grass

pathogen). In this research we propose to stably express and characterize this novel endolysin and

standardize conditions for expressing it in a model plant such as Tobacco leading to the development of

transgenic plants expressing phage endolysin against bacterial pathogens. (this research is currently

protected from publication for patent application).

A novel bacteriophage protein against gram negative pathogens (from 2012 – present): I have

discovered a novel protein from a bacteriophage isolated from agricultural soil that shows complete

lysis activity against various gram negative animal and plant pathogens. Critical investigation revealed

that this is a stomatin like membrane protein that might be interacting with the outer membrane of gram

negative bacteria by forming a lipid raft. Since endolysins are ineffective against gram negative

pathogens due to the presence of outer membranes which it cannot pass through to access cell wall, this

protein may serve as lysins against gram negative pathogens. Research is underway to understand the

molecular mechanism of how this protein interacts with outer membrane of gram negative bacteria.

(this research is currently protected from publication for patent application).

Developing methods for assessing bacteriophage diversity in soil (from 2010 – 2011): Helped in

developing a new approach to examining the composition of soil viral communities based on random

PCR amplification of polymorphic DNA (RAPD-PCR). A key methodological improvement was the

use of viral metagenomic sequence data for the design of RAPD-PCR primers. This 'metagenomically-

informed' approach to primer design enabled the optimization of RAPD-PCR sensitivity for examining

changes in soil viral communities. Overall, this work demonstrates that RAPD-PCR fingerprinting is an

inexpensive, high-throughput means for addressing first-order questions of viral community dynamics

within soil samples and thus fills a methodological gap between narrow single gene approaches and

comprehensive shotgun metagenomic sequencing for the analysis of viral community diversity.

(Published in Appl. Environ. Microbiol. 2013 (in press) ahead of print, online in 21 June 2013,

doi:10.1128/AEM.00268-13)

Construction and optimization of a microbial sensing system for quick assessment of soluble and

biologically unavailable organic phosphate in agricultural soils (from early 2013 – Present):

Management decisions regarding phosphorus amendments in agricultural soils are largely driven based

on empirically derived extractions that tend to be site specific and perform poorly in high organic

and/or manure-amended soils. The goal of this research is to develop a biologically-based soil-P test

through monitoring soluble soil phosphorus by using a whole cell biosensor, in a dose dependent

manner. Currently, research is being carried out to develop a recombinant strain that can be embedded

in a semi solid matrix in a close association with soil and while embedded the strain would produce

enzymes (such as phytase) externally to dissolve organic phosphate that can be sensed by a reporter

system such as phoA regulon constructed in the same strain. After development this reporter assay will

be useful to asses bioavailable soil-P in various agricultural soil.

Construction of a whole cell biosensor for polychlorinated biphenyles (PCBs) (from 2003 – 2004):

Polychlorinated biphenyls (PCBs) are toxic and hydrophobic compounds that are persistent in the

environment. Therefore detection of PCBs in environmental samples is increasingly important. To that

end, in this research, I have developed a whole cell sensing system harboring the plasmid pSD7000

which contains the reporter gene, under the control of the bph operon. The detection is achieved

through the emission of light afforded by the expression of reporter protein triggered by the presence of

biphenyl. (Published in Toxicol. Environ. Chem., 2005, 87, 3, 287-298)

Prevalence of lysogeny and horizontal gene transfer among soil bacteriophages (from 2005 – 2007):

Bacteriophages are believed to affect the activity and genetic diversity of bacterial communities in

aquatic environments but very little was known about lysogeny and transduction in the soil

environment. In this research we employed atrazine-impregnated Bio-Sep beads (a cell immobilization

matrix) to sample active microbiota from soils with prior pesticide exposure history. Once recovered

from soil, the bead communities were induced with mitomycin C (MC) to evaluate the incidence of

inducible prophage in soil bacteria. In contrast to aquatic system we found the inducible fraction within

the bead communities was very high. Moreover, the bacterial genes encoding 16S rRNA and trzN, a

chlorohydrolase gene responsible for dehalogenation of atrazine, were detected in the viral DNA

fraction purified from MC-induced bead communities. Heterogeneous 16S rRNA gene sequences

consisting of fragments from two different taxa were detected in the clone libraries which suggested

that lysogeny is a prevalent reproductive strategy among soil bacteriophages and that the potential for

horizontal gene transfer via transduction is significant in soil microbial communities. (Published in

Appl. Environ. Microbiol. 2008. 74: 495-502.).

Metagenomic data analysis of the Chesapeake Bay bacteriophage community (from 2005 – 2007):

Took part as a team member to analyze the metagenomic library constructed from Chesapeake Bay

virioplankton. The resulting sequences constitute the largest collection of long-read double-stranded

DNA (dsDNA) viral metagenome data reported at that time. This analysis suggested that dsDNA

viruses are likely one of the largest reservoirs of unknown genetic diversity in the biosphere.

Identification and analyses of cyanobacterial homologs of the psbA gene illustrated the value of

metagenomic studies of virioplankton. The phylogeny of inferred PsbA protein sequences suggested

that Chesapeake Bay cyanophage strains are endemic in that environment. The ratio of

psbA homologous sequences to total cyanophage sequences in the metagenome indicated that

the psbA gene may be nearly universal in Chesapeake Bay cyanophage genomes. Furthermore, the

result also showed that Chesapeake Bay cyanophage populations are dominated by Podoviridae.

(Published in Appl. Environ. Microbiol. 2007.73: 7629-7641).

In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-

containing porous beads (from 2006 – 2008): We examined the community composition of microbes

that colonized atrazine-containing beads buried in agricultural soils that differed in atrazine treatment

history. Bacterial abundance was greater in atrazine-fortified beads. In beads containing low atrazine

content and buried in soil with a history of atrazine application (conditioned soil), the abundance of

Actinobacteria increased whereas in control soil, the gamma-Proteobacteria and Planctomycetes

increased remarkably. The gamma-Proteobacteria were enriched in high amount in beads containing

high atrazine content which was buried in conditioned and control soil as well. The results demonstrate

that BioSep® beads are a suitable matrix for recruiting a diverse subset of the bacterial community

involved in atrazine degradation. (Published in, Soil Biol. Biochem. 2009. 41:1331-1334)

Characterization of atrazine degrading cultures from Nigerian agricultural soil using Bio-Sep bead

enrichment techniques (from 2008 – 2009): Helped and mentored a Nigerian student to characterize

atrazine degrading bacterial community by using the Bio-Sep beads, a new approach of enriching

microbial community in situ. This research reports community-level biodegradation of atrazine using

consortia developed via in situ enrichment technique from a Nigerian agricultural soil with long history

of atrazine contamination. The consortia were subsequently characterized using genetic fingerprinting

technique and screened for known atrazine catabolic genes. (Published in Appl. Soil Ecol. 2011. 48 (1):

63–70)

Type VI secretion systems (T6SS) as modulators of plant-microbe associations (from 2011 – 2012):

Recently, I have discovered that acyl homoserine lactones (AHLs), the essential signaling molecules of

quorum sensing in many Gram-negative bacteria, can trigger bacteriophage production in

Sinorhizobium, soil and groundwater bacteria (Ghosh, et al., 2009) (see peer reviewed publication).

This finding suggest an alternative paradigm for prophage induction based not on DNA damage but

rather on signals that reflect the presence of a high density of host bacteria under otherwise

environmentally favorable conditions (e.g. the rhizosphere). Type VI secretion systems (T6SS), on the

other hand are widespread in bacteria and has been revealed to be a “degenerated bacteriophage” which

has lost its ability to lyse the host cell during evolution and the host may have repurposed its function

as secretor for connecting cells. However, the environmental signal that might trigger the expression of

T6SS in rhizobacteria is unknown. Given the fact that T6SS is a “degenerated bacteriophage” and our

new finding of quorum sensing mediated bacteriophage induction we hypothesized that T6SS

expression in rhizobacteria would be triggered by quorum sensing compounds and indeed, we found

correlation between indole 3 acitic acid concentration and T6SS expression in Azospirillum brasilense.

We have constructed T6SS mutants of Azospirillum brasilense which shows different coagulation

behavior from wild type. Currently research is underway to determine these behaviors in response to

quorum sensing compounds such as indole and acyle homoserine lactones and evaluate their role in

nodulation and plant growth promotion. (This research was initially funded by the M-CERV program of

the University of Tennessee and currently in preparation for submission to external agency)

DOCTORAL RESEARCH EXPERIENCES

Molecular Phylogenetic analysis and metabolic characterization of Bacterial diversity in an Indian

hot spring (from 1998 – 2003): The bacterial diversity of a hot spring in India, was investigated by a

culture-independent approach. A 16S ribosomal DNA clone library was constructed from the hot spring

sediment samples. Sequences of these clones were revealed to be associated with gamma-

Proteobacteria, cyanobacteria, and green nonsulfur and low-GC gram-positive bacteria. In the

phylogenetic tree constructed from the sequences of the library, the gamma-Proteobacterial sequences

cobranches with Shewanella, a well-known iron reducer but never known to be thermophilic organisms.

This phylogenetic correlation of thermophilic Shewanella and iron reduction has been exploited to

develop culture conditions for thermophilic iron-reducing microorganisms. (Published in Appl.

Environ. Microbiol. 2003. 69: 4332-4336)

AWARDS AND COMMENDATIONS

Best Publication highlighted in “Microbe” (News magazine published by the

American Society for Microbiology) 2008

Junior Research Fellowship awarded by the

Department of Atomic Energy (DAE), Govt. Of India 1999-2003

PROFESSIONAL AFFILIATIONS & ACTIVITIES

Member, American Society for Microbiology (ASM) and International Society for Microbial Ecology

(ISME)

Ad hoc reviewer for the following journals,

Microbial Ecology

•

FEMS Microbiology Ecology

•

Soil Biology and Biochemistry

•

Canadian Journal of Microbiology

•

BMC Microbiology

•

Biology and Fertility of Soils

•

REFERENCES

Mark Radosevich (Present Supervisor)

Professor

Department Of Biosystems Engineering & Environmental Science

Plant Biotech Unit

University Of Tennessee, TN 37996, USA

Email: ****@***.***

Phone: 865-***-****

Gladys Alexandre

Associate Professor

Department of Biochemistry, Cellular and Molecular Biology

F425 Walters Life Sciences Building

1414 W. Cumberland Avenue

Knoxville, TN 37996

Ph: 865-***-****

Fax: 865-***-****

Email: ********@***.***

David A. Golden

Professor, Food Microbiology

President-elect, UTK Faculty Senate

The University of Tennessee

Department of Food Science and Technology

2510 River Drive

Knoxville, TN 37996-4539

phone: 865-***-****

fax: 865-***-****

email: ***@***.***

Kurt E. Williamson

Assistant Professor

The College of William and Mary

Department of Biology

2127 Integrated Science Center

Williamsburg, VA 23187

Phone: 757-***-****

Email: ************@**.***

Ramesh Goel

Associate Professor

Civil & Environmental Engineering

University Of Utha

122 S. Central Campus Dr., Suite 104

Salt Lake City, UT 84112

Phone Number: 801-***-****

Fax Number: 801-***-****

Email: *****@*****.****.***

Subrata Pal (PhD thesis supervisor)

Professor & Dean (Faculty of Science, JU)

Department of Life Science and Biotechnology

Jadavpur University

Kolkata-700032, India

email: *********@*******.***

Phone: +91-33-247***** (Res)

Contact this candidate