Project Management

MANASI BARATH

**** ****** ***** *****, *** P*, San Jose, CA - 95119* 408-***-***** ******.******@*****.***

Education:-

University of Southern California (USC), Keck School of Medicine, Los Angeles, CA 2011 - 2013

Master of Science - Molecular Microbiology & Immunology

Courses: Molecular Biology of Cancer, Infection & Host Responses, Introduction to histology/ Pathology, Cell

Biology, Biochemistry, Biostatistics, Structure and Management of Clinical Trials.

Dr. D. Y. Patil University – Dept. of Biotechnology & Bioinformatics, Navi Mumbai, India 2007 - 2011

Bachelor of Technology – Biotechnology

Courses: Immunology, Microbiology, Molecular Biology, Pharmacology, Animal & Plant Cell Culture, Genetic

Engineering, IPR, Bio-safety, Bioethics and Entrepreneurship.

Qualification Summary:

Masters graduate from USC in Molecular Microbiology and Immunology with a background of pharmacological

research in breast cancer and highly motivated to work in the field of applied biology for medicine and patient care.

Technical Skills:-

Cell Culture:

Culturing of cancer cells (human as well as non-human cells)

Colony forming assay

Culturing of tumor initiating stem cells

Scratch-wound assay

Isolation of PBMCs (buffy coat) from whole blood samples

Isolating culturing and staining bacterial samples

Molecular / Cellular Biology & Immunological Techniques:

Western Blotting

Electrophoresis (Agarose, SDS-PAGE)

MTT Assay

FACS

Plasmid Transfection

Plasmid DNA Isolation using Miniprep

shRNA/siRNA Transfection

ELISA

Protein, Nucleic Acid Quantification

RT-PCR

PCR

Immunofluorescence and Confocal Microscopy

Immunohistochemistry

H&E Staining of tissue sample

Karyotyping using Ikaros (Meta-Systems, Germany)

Computer skills:

Proficient in Graphpad Prism, Microsoft Office

Exposure to bioinformatics software such as Rasmol, Swiss pdb, Ligand Scout, Marvinsketch, DS Visualizer and

Argus Lab.

Other:

Inventory management for lab supplies.

MANASI BARATH

6181 Camino Verde Drive, Apt P3, San Jose, CA - 95119* 408-***-***** ******.******@*****.***

Research Experience:-

Dr. Axel.H Schönthal’s laboratory at Keck School of Medicine of USC - Master’s Thesis Jan 2012 – Jun 2013

The project involved pre-clinical trials of a novel anti-cancer drug candidate on breast cancer cell lines. The research

focused on discovering the mechanism of action, potency, and mechanism of uptake and other characteristics of the

compound. The long-term aim is treating breast cancers in the breast as well as those that have metastasized to the

brain. This project helped me grow as a researcher and think outside the box. It got me accustomed to working

individually while also taught me the value of a team.

Cytogenetic Analysis of Acute Lymphoblastic Leukemia (ALL) at MGM hospital, Navi Mumbai, India –

Undergraduate Thesis Jan 2011 – June 2011

The project was under the guidance of Dr. Bani. B. Ganguly towards the partial fulfilment of Bachelor’s degree. The

project involved karyotyping of bone marrow samples of ALL patients followed by additional cytological analysis of

the sample. Karyotyping was done following G-banding of chromosomes in order to determine chromosomal

aberrations.

Publications

A publication is underway from the current research work.

I co-authored two abstracts on ‘Genetic basis of Rhabdomyosarcoma’ and on ‘Chromosomal abnormalities in

follicular thyroid carcinoma’ which were selected for publication in the European Society of Human Genetics

Conference 2011.

Other Strengths:

Highly passionate about research owing to history of cancer in the family.

Strong team player – was a source of motivation to peers during lack of progression in research

Co-curricular & Extra-curricular Highlights:-

Completed short term certification course in Responsible Conduct of Research conducted at USC - 2013

Presented a poster of research findings in the annual Microbiology and Immunology meet at USC. The event was host

to all department researchers and students. -2012

Completed Short Term Certification Course in Clinical & Translational Science conducted by SC CTSI, USA - 2012

Attended training conducted by Onan Biotech Pvt. Ltd. which gave hands- on training in various molecular biology

practical techniques like RT-PCR, RFLP, 2D gel electrophoresis, etc. – 2011

Attended the National Seminar on Frontiers in Biotechnology. - 2010

Trained at the Microbiology department of the Pathology section of Jupiter Hospital, Thane. - 2010

Trained at MM medical center and diagnostics, Mumbai, under the histopathology and microbiology department. –

2011

Participated in the Mumbai Marathon for a charitable organization that works for terminally ill children. – 2010

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