Analytical Chemist (GCMS, LCMSMS)

RAJ K. BHANDARI

**** *** ******, *** # * Brookings, SD 57006

Phone: 320-***-****

Email: ***.********@*******.***

ANALYTICAL CHEMIST

SUMMARY: Over 4 years experience with GC-MS, UHPLC-MS-MS, HPLC and ICP-MS

method development and method validation for small molecules analysis. Skilled at performing a

variety of routine and non-routine complex scientific tests including troubleshooting, installation,

and maintenance of GC-MS, HPLC, UHPLC-MS-MS and ICP-MS. Personal strengths include

problem-solving skills, ability to work within an interdisciplinary team, and excellent oral and

written communication.

INSTRUMENTAL EXPERIENCE

GC-MS: Agilent Technologies 5975 Series

UHPLC-MS-MS: ABSCIEX QTRAP 5500 LC/MS/MS System

HPLC: Agilent Technologies 1200 Series

ICP-MS: Perkin Elmer Elan 6100 Series

UV/VIS, FT-IR, pH-meter

EDUCATION

August 2013 Ph.D. in Chemistry (Analytical Chemistry)

South Dakota State University (SDSU), Brookings, SD

Dissertation title “The toxicokinetic behavior of cyanide, thiocyanate, and 2-amino-2-

thiazoline-4-carboxylic acid (ATCA) following cyanide exposure in multiple animal

models utilizing novel methods of detection.”

Advisor: Brian A. Logue, Ph.D.

May 2008 Bachelors of Science in Chemistry (ACS), Cum Laude

Minor in Biology, Saint Cloud State University (SCSU), St. Cloud, MN

Advisor: Michael A. Dvorak, Ph.D.

RESEARCH/ PROFESSIONAL EXPERIENCE

July 2009 - August 2013 Graduate Student, Department of Chemistry and Biochemistry,

SDSU, Brookings, SD

• Developed and validated a bio-analytical method for simultaneous determination of

cyanide, thiocyanate and ATCA using ultra-high performance liquid chromatography

tandem mass spectrometry (UHPLC-MS-MS) and HPLC with fluorescence detector and

by chemical ionization gas chromatography mass-spectrometry (CI-GC-MS)

• Performed toxicokinetic analysis of cyanide and its detoxification products in cyanide

exposed rat, rabbit and swine bio-fluids (blood and plasma) with liquid-liquid extraction,

solid phase extraction, and protein precipitation sample preparation procedures

July 2008 - June 2009 Research Assistant, Harvard University, School of Public Health,

Boston, MA

Raj K. Bhandari, ***.********@*******.***, 320-***-****

• Rendered key input in the development and validation of analytical test procedures using

ICP-MS for trace metal analysis of arsenic and manganese from hair and toenail samples

May 2008- July 2008 Analytical Chemist I, Paddock Labs (Now Perrigo), Minneapolis, MN

• Performed validation on OTC drug (e.g., Bisacodyl) using dissolution assay and HPLC

• Participated in cross-functional teams at project-related meeting and technical discussions

to resolve problems with suppliers, methods and project issues

LEADERSHIP ROLES

• Senator, SDSU Students’ Association (2012-2013)

• Students’ Association representative, Student Summit on Diversity & Inclusion (2013)

• SDSU Student Representative, SDSU International Committee (2011-2013)

• Student Representative, SDSU Library Committee (2012-2013)

• President, SDSU Nepalese Students’ Association (2011-2012)

PUBLICATIONS (in reverse chronological order)

5. Bhandari, R. K., Oda, R. P., Petrikovics, I., Thompson, D. E., Brenner, M., Mohan, S. B.,

Bebarta, V. S., Rockwood, G. A., and Logue, B. A. (2013) Cyanide Toxicokinetics: The Behavior

of Cyanide, Thiocyanate and 2-Amino-2-thiazoline-4-carboxylic Acid in Multiple Animal

Models. In Preparation for Submission to Toxicology Letters

4. Bhandari, R. K., Manandhar, E., Oda, R. P., Rockwood, G. A., and Logue, B. A. (2013)

Simultaneous high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-

MS) analysis of cyanide and thiocyanate from plasma. Submitted to Anal Bioanal Chem

3. Mitchell, B.L., Bhandari, R. K., Bebarta, V.S., Rockwood, G.A., Boss, G.R., and Logue, B.

A. (2013) Toxicokinetic profiles of α-ketoglutarate cyanohydrin, a cyanide detoxification

product, in swine after exposure to potassium cyanide. Toxicology Letters, 222, 83-89

2. Bhandari, R. K., Oda, R. P., Youso, S. L., Bebarta, V. S., Petrikovics, I., Rockwood, G. A.,

and Logue, B. A. (2012) Simultaneous determination of cyanide and thiocyanate in plasma by

chemical ionization gas-chromatography mass-spectrometry (CI-GC-MS). Anal Bioanal Chem,

404 (8), pg. 2287-2294

1. Petrikovics, I., Yu, J. C. C., Thompson, D. E., Jayanna, P., Logue, B. A., Nasr, J., Bhandari,

R. K., Baskin, S. I., Rockwood, G. (2012) Plasma Persistence of 2-Aminothiazoline-4-

Carboxylic Acid in Rat System Determined by Liquid Chromatography Tandem Mass

Spectrometry. Journal of Chromatography B, 891-892, pg. 81-84

SELECTED PRESENTATIONS (6)

2. Bhandari, R.K., Oda, R.P., Petrikovics, I., Bebarta, V.S., Mohan, S.B., Brenner, G.A.,

Rockwood, Logue, B.A. Toxicokinetics of cyanide, thiocyanate and 2-amino-2-thiazoline-4-

carboxylic acid (ATCA) by simultaneous analysis using Gas Chromatography Mass-

Spectrometry (GC-MS). 245th American Chemical Society National Meeting & Exposition, New

Raj K. Bhandari, ***.********@*******.***, 320-***-****

Orleans, LA, April 7-11, Oral Session, Division of Analytical Chemistry, Advances in

Biopharmaceutical and Pharmaceutical Analysis, ANYL 15, 2013

1. Bhandari, R. K., Oda, R. P., Petrikovics, I., Bebarta, V. S., Mohan, S. B., Rockwood, G. A.,

and Logue, B. A. Assessing the toxicokinetic behavior of cyanide, thiocyanate, and 2-amino-2-

thiazoline-4-carboxylic acid (ATCA) following cyanide exposure in multiple animal models.

18th Biennial Medical Defense Bioscience Review, Hunt Valley, MD, USA, May 20-May 24;

Poster 78, 2012

HONORS AND AWARDS

• Sigma Xi Graduate Research Competition, 1st position, Ph.D. paper presentation (2013)

• Logue Chemistry and Biochemistry Graduate Student Award (2012)

• Recipient, ACS Awards Grants for International Year of Chemistry (IYC) (2011)

• SDSU Graduate School Travel Award (2011)

• Recipient, ORISE Fellowship (2010-2011)

• Cum Laude, St. Cloud State University (2008)

• Recipient, International Merit Scholarship (2004-2008)

PROFESSIONAL AFFILIATIONS

• Member, American Society for Mass Spectrometry (ASMS) (2013-Present)

• Member, American Chemical Society (ACS) (2009-Present).

• Member, Phi Lambda Upsilon, Chemistry Honor Society (2008-Present)

• Member, International Society of Poets (2007-Present)

SYNERGISTIC ACTIVITIES

• Taught general chemistry I and II, organic and biochemistry I and CHEM 332 (Analytical

chemistry) labs (2009, 2012-2013)

• Reviewed an article for Journal of Chromatography B, 2012

• Successfully completed the leadership and team building ‘The Soderquist Center’

workshop (2012)

• Served as a judge for state and regional high school science fair (2005-2008)

REFERENCES (Others available upon request)

• Dr. Brian A. Logue, Associate Professor, Dept. of Chemistry and Biochemistry,

South Dakota State University, *****.*****@*******.***; Phone: 605-***-****

• Dr. Jihong Cole-Dai, Professor, Dept. of Chemistry and Biochemistry, South Dakota State

University, ******.****-***@*******.***; Phone: 605-***-****

• Mr. Robert P. Oda, Lab. Manager, Logue Group, Dept. of Chemistry and Biochemistry,

South Dakota State University, ******.***@*******.***; Phone: 605-***-****

• Dr. Michael A. Dvorak, Associate Professor, Dept. of Chemistry, St. Cloud State

University. ********@************.***; Phone: 320-***-****

Raj K. Bhandari, ***.********@*******.***, 320-***-****

Contact this candidate