Quality Control Assistant

Sita Dahal Ghimire

*** ******** *** #**, ******, MA- 02148

512-***-****, email: ****.*****@*****.***

SUMMARY

A Biotechnology professional having strong academic performance in Biotechnology

and Molecular Biology and experience in research and in-vivo and in-vitro analysis at

laboratories, seeking job opportunities in the Pharmaceutical, Biotech, IVD or Medical

Device Manufacturing, Research, Development and QC Laboratory environment.

EDUCATION

Masters in Molecular Biology

University of Gothenburg, Sweden (VG in Master’s thesis) 2011-2013

Courses: Molecular Biology (VG), Experimental Systems Biology (G), Statistical

Analysis and Experimental Design (G), Bioinformatics and Functional genomics

(gene expression analysis including microarray analysis) (VG), Master’s thesis work

(60 credit hours)

Masters in Biotechnology

Sathyabama University, India (GPA: 3.8) 2006-2008

Courses: Immunology and advanced Immunotechnology, Genetics, Techniques in

Molecular biology and Genetic Engineering, Biology of Cloning vectors, Cell Biology,

Fermentation Technology, Enzyme Kinetics, Bio-Chemistry, Statistics, Bio-Physics,

Animal Cell Culture, Animal handling, Pharmaceutical Biotechnology, Microbiology,

Microbiology laboratory.

Bachelor of Science

Andhra University, India (GPA: 3.5) 2003-2006

Courses: Chemistry, Bio-Chemistry (clinical Bio-chemistry), Biotechnology

PROFESSIONAL EXPERIENCES

1. Project

Lundberg Laboratory, University Of Gothenburg, Sweden Sep 2011-Sep 2012

• In-vitro and in-vivo analyses of Saccharomyces cerevisiae mitogen activated

protein kinases – validation of a novel peptide based screening for protein

kinase inhibitors and study of Slt2-Fps1 (protein-protein) interaction

• Techniques employed: Transformation, Plasmid preparation, Protein

expression (membrane and cytosolic proteins) in heterologous and

homologous system, affinity purification, SDS-PAGE, Cloning, mutagenesis,

protein quantification assays, Western blot, Southern blot, Immuno-

precipitation, in-vivo and in-vitro protein phosphorylation assay, enzyme assay

in multi-well plate-based format (96), Data analysis, radiometric assays,

confocal microscopy

2. Internship

VIB Laboratory of Systems Biology, K.U. Leuven, Belgium Jun 2011- Aug 2011

• Crossing of the industrial Saccharomyces cerevisiae strains to increase

ethanol tolerance

• Techniques employed: Sporulation, dissection of spores, large scale crossing

of yeast strains, PCR ( theoretical knowledge of qPCR analysis) & agarose gel

electrophoresis and growth inhibition assays

3. Teaching Assistant

School of Biotechnology, Himalayan Whitehouse College of Science and Engineering

(Purbanchal University), Nepal July 2008-Aug 2010

• Managed laboratory and course work for Molecular Biology (animal handling)

& Immunology

• Mouse handling and tissue harvesting, RNA extraction and quality control

using Nanodrop and Experion, RT-PCR including evaluation and analysis of

results, Mammalian tissue culture, cell based assays, Western blot, ELISA

(washing steps carried out automatically by liquid handling systems),

Immunohistochemistry

4. Teaching Assistant

Department of Biotechnology, Lord Buddha Education Foundation, Nepal

Feb 2010-Jul 2010

• Responsible for guiding, evaluating and assessing the progress of students

5. Under-Graduate Project

Biomimetic Synthesis of Polymer Nanocomposites

National Metallurgical Laboratory (Council for Science & Industrial Research), India

Dec 2007-Mar 2008

• Techniques employed: Chemical synthesis of nanoparticles using proteins and

polymers as the templates, Scanning Electron Microscopy, Atomic Force

Microscopy, X-Ray Diffraction, Spray drying, Ultraviolet–visible spectroscopy

POSTERS, PRESENTATIONS & PUBLICATIONS

Manuscript under preparation

• Veide Vilg J, Dahal S, Grotli M, Tamás MJ (2012) “A peptide-based method for

accurate and convenient characterization of protein kinase inhibitors.”

Manuscript in thesis

• D. Ahmadpour, Roja B, Ewa M.D., S. Dahal, Robert W, Tamás MJ, Hohmann

S (2012) “ The MAP kinase Slt2 modulates transport through the

aquaglyceroporin Fps1.” Manuscript in doctoral thesis “Stress, homeostasis

and robustness: molecular and systems-level analysis in yeast” by Doryaneh

Ahmad Pour, Dept. of Chemistry and Molecular Biology, University of

Gothenburg.

(https://gupea.ub.gu.se/bitstream/2077/28374/1/gupea_2077_28374_1.pdf)

• Co-authored Issue topic “Biotechnology: Past, present and future-Nepal” at

symposium organized by the Bangladesh Academy Of Science, 2010

• ”Protein profile of the different variety wheat’s found in varied regions of Nepal”

poster presentation at the International Biotechnology Symposium organized

in Nepal, 2009

TRAININGS

• Completed intensive training/workshop on “Basic health systems research

methodology (proposal development)” by Nepal Health Research Council on

July 17-21, 2010

• Industrial training programme on Genomics, 2 weeks programme (5th June-

19th June 2007)

HONORS AND AWARDS

• Adlerbert Foreign Student Hospitality Foundation, 2011 (university of

Gothenburg)

• TATA Golden Jubilee Scholarship, 2007-2008

COMPUTER SKILLS

Microsoft Office Applications, R, Cell Designer, Cytoscape, MATLAB (beginner)

REFERENCES

1. Markus J Tamas (Professor)

Lundberg Laboratory (Sahlgrenska Academy), University of Gothenburg

email: ******.*****@***.**.**

2. Jenny Veide Vilg (Fil.Dr. PhD)

Institutionen för Kemi- och bioteknik, Chalmers University of Technology

email: *****.****@********.**

3. Kevin Verstrepen (Professor)

CMPG - VIB Laboratory of Systems Biology, KU Leuven

*****.**********@***.***-********.**

3. Jeanette Nilsson (Senior Lecturer)

Master’s Programme Co-Ordinater

Department of Biochemistry & Molecular Biology, University of Gothenburg

email: ********.*******@***.**.**

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