Medical Technician
Resume:
Dr. Tong Zhang
**** ******* *** **. ****** Mound, TX 75028
Phone: 972-***-****
E-mail: ************@*******.***
Objective goal
To obtain a histological technician or medical laboratory technician
position where I can continue applying my knowledge and skills in bio-
medical researches or medical care service.
Summary of qualifications
I have broad knowledge in medicine and pathology. I also have working
experience of gross surgical pathology, and histology and
immunohistochemistry. I also have research experience and team work
experience. I have mastered many basic and special research techniques
in biochemistry and molecular biology and cell biology, and histology.
These will support me to feed in the job.
Expertise/ Competencies
I am good at gross examination in surgical pathology, and I am familiar
with techniques in tissue fixation, tissue process, embedding, tissue
cutting, histochemistry and immunohistochemistry. I am comfortable with
photography under microscope. Besides, I am strong with techniques in
biology and molecular biology. I am also a good trouble shooter.
Employment History
July,2009-May,2012: Research Associate, UT Southwestern Medical Center,
Dallas, TX August, 2002-June, 2009: Research Associate, Health Science
Center, University of Manitoba, Winnipeg, Manitoba
May, 2000-August, 2002: Visiting Research Fellow, NIEHS, National
Institutes of Health, Durham, NC
June, 1996-May, 2000: Instructor and Associated Professor, Fourth Military
Medical University, China
March,1986-August,1993: Resident and Instructor, Xi'an Medical University,
China
April,1989-June,1990: Visiting research associate, University of Chicago
Working Experience
07/09-05/12, Research Associate, UT Southwestern Medical Center at Dallas,
Texas
( Successfully purified cMLCK and pRLC proteins from CMLCK KO mouse heart
tissue for sequence analysis, and found several novel phosphoralation
sites in N terminal of these two proteins. These results are suggestive of
some new potential regulatory sites in these two proteins which control
the stroke power of the heart contraction.
( Studied LZTFL-1 gene inhibitory effects on EMT in human lung cancer cell
lines
In the research, several non-small cell human lung cancer cell lines which
are usually lack of LZTFL-1 expression, were stably transfected with LZTFL-
1 gene. After the transfection and TGF?1 treatment, real time PCR showed
that mRNA level of Snail1 downstream EMT makers TCF4 and SPARC was
significantly down regulated, and sort agar assay showed that tumor clone
forming capacity was significantly reduced in the cancer cells. These
results proved inhibitory effects of LZTFL-1 on epithelial-mesenchymal
transition(EMT) in the lung cancer cells. And LZTFL-1 gene therapy could
be a potential remedy for lung cancer.
08/02-06/09, Research Associate, Health Science Center, University of
Manitoba, Winnipeg, Manitoba, Canada
(Researched on apoptotic signaling in cardiomyocytes, mainly P53 and BNip3
and NF(B p65 in mitochondrium pathways
This research showed the cell death protein BNip3 interact with p53 and
facilitates p53 anchoring in to mitochondrium. The two proteins interact
with VDAC and open the PT pore to release cytochrome C to initiate cell
apoptosis. Targeting this pathway and blocking the PT pore opening could
be a potential remedy in heart failure.
05/00-08/02, Visiting Research Fellow, NIH/NIEHS, Durham, NC
( Purified native inositol phosphate 1-phosphatase, Ins(13456)P5 1-
phosphatase from Mipp-knock out transgenic mouse liver and Studied and
characterized a novel reversible inositol phosphate kinase/phosphatase,
which regulate Ins(3456)P4 signaling
07/96-05/00, Instructor, Associated professor, Fourth Military Medical
University, China
( Taught general pathology and did surgical pathology examination
( Screened hepatoma apoptotic cell cDNA library and obtained several cDNA
related to the apoptosis of the hepatoma cells
( Characterized antitumor effects of the herb medicine Microula Sikklmensis
hemsli
04/39-06/90, Research associate, Zoller Dental Clinic, University of
Chicago, Chicago, IL
( Did surgical oral pathology, gross and microscopic examination
( Did tissue processing, microtomy and staining.
( Studied and reviewed slides of difficult cases
03/86-08/93, Resident, Instructor, Xian Medical University, China
( Taught oral embryology and oral pathology
( Did histotechnological work, such as tissue processing, embedding,
microtomy and histochemical staining
( Conducted surgical oral pathology examination and clinical pathological
studies
( Studied epidemics of human papilloma virus in oral cancer using PCR and
DNA hybridization.
Technical experience and skills
( Tissue fixation, tissue processing, tissue embedding, microtomy,
histochemical staining and immunohistostaining. Light microscopy and
fluorescence microscopy
(Western blot, immuno-precipatation, Elisa, and immunohistochemistry
(Gene cloning to construct vectors, gene expression in E.coli or in
mammalian cells, and making vitrovirus for stable transfection, adenovirus
growing and virus titer assay, protein purification from mammalian tissue
and from E.coli, FPLC, HPLC, PCR, Real time PCR and Southern blot, antibody
conjugation
(Cell culture, cell line and primary cell culture, e.g. isolation of adult
and neonatal cadiomyocytes, and isolation of mouse cardiomyxoma tumor
cells.
( Skillful in small animal handling, genotyping, injection and surgery in
animals. Surgery in rabbits( for three years).
Education
Master in Medicine, Xi'an Medical University, China
B.S in Medicine, Xi'an Medical University, China
Publications
1. Zhang T and Kirshenbaum LA. Over Expression of Bnip3 increases mTOR
activity and facilitates myoxoma growth in mouse hearts. (to be published)
2.Shaw J, Yurkova N, ZhangT, Gang H, AguilarF, Weidman D, Scramstad C,
Weisman H, Kirshenbaum LA. Antagonism of E2F1 regulated transcription by NF-
kappaB is essential for basal cell survival. Proc Natl Acad Sci USA 2008
Dec30;105(52):20734-9. Epub 2008Dec 16. PMID: 19088195
3. Shaw J, Zhang T, Rzeszutek M, Yurkova N, Beatz D, Davie JR, Kirshenbaum
LA. Transcriptional silencing of the death gene BNip3 by cooperative action
of NF-kappaB and histone deacetylase 1 in ventricular myocytes. Circ Res.
2006 Dec 8;99(12):1347-54 . Epub 2006 Nov 2. PMID: 17082476 [PubMed-
indexed for Medline]
4. Pesesse X, Choi K, Zhang T, Shears SB. Signaling by higher inositol
polyphosphates synthesis of bisdiphosphoinositol tetrakisphosphate ("Ins
P8") is selectively activated by hyperosmotic stress. J Biol Chem 2004 Oct
15;279(42)43378-81.
5. Chen L, Zhang T, Nyomba BL. Insulin resistance of gluconeogenic
pathways in neonatal rats after prenatal ethanol exposure. Am J Physiol
Regul Integr Comp Physiol. 2004 Mar;286(3):R554-9. Epub 2003 Nov 13. PMID:
14615274 [PubMed - indexed for MEDLINE]
6. Hidaka K, Caffrey JJ, Hua L, Zhang T, and Shears SB et al. An adjacent
pair of human NUDT genes on chromosome X are preferentially expressed in
testis and encode two isoforms of diphosphoinositol polyphosphate
phosphohydrolase. J Biol Chem 2002 Sep6;277(36):32730-8.
7. Tong Zhang, James J Caffrey, Stephen B Shears. The Transcriptional
regulator, Arg82, is a hybrid kinase with both monophosphoinositol and
diphodphoinositol polyphosphate synthase activity. FEBS Letters
24783(2001)1-5.
8. Zhang Tong, Wang Wenliang, et al. Construction of human adeno-associated
virus type 2 (AAV-2) based vectors and expression of neo and IL-2 genes in
mammalian cells. Journal of Xian Medical University, 1997;18(3):312-316.
9. Zhang Tong, Chu Yonglie and Yuan Yukang, et al. A study on the
relations between human papilloma virus and oral cancers with the means of
nucleic acid hybridization. Chinese Joural of Stomatology, 1995;30:37-39
10. Zhang Tong, Chu Yonglie and Yuan Yukang, et al. Detection of human
papilloma virus type 16 E6 gene related sequence in oral cancers with the
means of polymerase chain reactions. Journal of Xian Medical University,
1994;15:8-11
References
Available upon request
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