Project Manager Assistant

Julia Lamenzo Fox, Ph.D.

Arlington, MA

Phone: 617-***-**** Email: *****.*.***.***@*****.***

Summary of Qualifications

. Research Scientist with >10 years of experience in high throughput and

high content chemical screening, including gene expression profiling

and analysis, assay development, genomics and drug discovery focused

on cancer stem cell biology, stem cell differentiation, primary cell

replication and viability

. Therapeutic / disease area experience includes Diabetes, Cancer,

Inflammation, Chemotherapy induced neuropathy and other

neurodegenerative diseases

. Implemented experimental design and lab set-up, coordinated the

research efforts of several projects and participating researchers and

directed fund raising, budget development, and project and personnel

management

. Strong interpersonal skills and dedication

Professional Experience

Novartis Institutes for Biomedical Research, Cambridge, MA

2013-present

Scientific Data Curator (Contractor), NIBR-IT

Massachusetts General Hospital and Harvard Medical School, Boston, MA

20122012-present

Assistant Investigator/ Instructor Cutaneous Biology Research Center, Dept.

of Dermatology

Chemical Biology Program Manager

. Responsible for set-up and operation of a chemical screening program

for dermatological applications related to skin cancer, skin pigment

and inflammation

. Assisted PI's in the design & execution of high throughput & high

content chemical screens, and managed hit calls and follow-up studies

for hit validation and target ID

. Developed relational database for chemical profiling studies

. Research techniques used include: high-throughput RT-PCR, automated

sample preparation and processing, computational data processing and

analysis, cheminformatics

Tufts University School of Medicine, Boston, MA

2010-2012

Assistant Investigator/ Instructor, SEMC Center for Cancer Systems Biology

. Responsible for scientific writing, grant & publication preparation

and review, summary communications and PR related briefs

. Responsible for the establishment of gene expression profiling

programs

. Developed cancer systems specific gene lists and inter-experimental

annotation of cancer related markers. Developed multi-gene signatures

of cancer development and progression stages using a systems approach

. Research techniques used include: Whole genome gene expression

profiling, whole genome methylation profiling, genome-wide association

studies and integrated analyses (Ingenuity, GenePattern and Cytoscape)

Harvard University, Department of Stem Cell and Regenerative Biology

2007-2009

Chemical Screening Research Associate / Co-PI, in collaboration with Dr.

Doug Melton

. Oversaw b?-cell proliferation assay development for chemical screening

for diabetes

. Research planning and project development, scheduling and execution

. Procured fresh human, rat and mouse isolated pancreatic islets and b ?-

cells

. Directed the experimental approach and miniaturization of primary b ?-

cell handling and measurement for high-throughput experiments (a team

of 3 FTEs and 10+ collaborators)

. Research techniques used include: coordination with on-site, as well

as public and private tissue donor and processing sites, primary

evaluation of ?-cell identity, viability and function (ELISA)

Broad Institute, MIT Chemical Biology Program, Harvard Stem Cell Institute

2004-2007

Stem Cell Chemical Screening Project Manager

. Responsible for the acquisition and coordination of funding from

public and private and philanthropic sources, screening laboratory and

experimental set-up, and on-going experimental design, execution and

analyses

. Project Management, personnel and staff management (3-4 FTEs and

numerous grad students and post-docs), resource and budget management,

long-term goal oriented on-target project evaluation and completion

. Directed efforts to screen for compounds capable of directing b ?-cell

development from human and mouse stem cell cultures toward a cellular

replacement therapy and drug discovery for Diabetes

. Research techniques used include: embryonic stem cell culture and

differentiation, high-throughput chemical screening techniques

including automated microscopy and image analysis (Cellomics,

Metamorph), high-throughput gene expression profiling and multiplexed

gene expression screens (Illumina, Affymetrix, Luminex)

Broad Institute Program in Chemical Biology, Cambridge, MA

2001-2004

Senior Assay Specialist, Supervisor: Dr. Paul Clemons

. Responsible for the design, development and implementation of cell-

based assays for high throughput chemical biology screening

. Developed general Cell Biological and Gene Expression assays to

address specificity and potency of chemical collections, and to serve

as background assays for specific biological processes or diseases

. Participated in the development of cheminformatics annotation and

screening database Chembank (http://chembank.broadinstitute.org)

. Research techniques used include: General cell biology and tissue

culture, systematic high-throughput techniques (plate readers and

automated handling), analyses using tools such as GenePattern,

PipelinePilot and Spotfire

FlyBase-Harvard, Harvard University, Cambridge, MA

1999-2001

Scientific curator, Supervisor: Dr. William Gelbart

. Responsible for molecular curation and computational annotation of

fruit fly genes for import into FlyBase (www.flybase.org),

specifically involved with import of 'legacy data' from the Redbook

and early molecular biology reports (pre-1990s)

. Computational gene annotation of the first fully sequenced genome

using in-house software and compiled sequence data

Brown University, Providence, RI 1994-

1999

Graduate student, MCB Department Advisor: Dr. Kristi A. Wharton

. Research focused on studying the role of a Drosophila melanogaster

TGF /BMP gene, gbb-60A, in larval and pupal development

. Teaching assistant for developmental biology course at Brown

University (1996-1998)

. Research techniques used include: genetics, genetic screens,

transgenic development (eg. FLP-FRT and GAL4-UAS systems), molecular

biology, enzymatic assays, and microscopy

Los Alamos National Laboratory, Los Alamos, NM

1993-1994

Graduate research assistant, Stable Isotope Resource, Supervisor: Dr. Louis

A. (Pete) Silks

. Developed step-wise biochemical syntheses of stable isotope labeled

nucleotides produced for NMR studies of structural and binding

elements in DNA

. Research techniques used include: Bio-organic enzymatic syntheses,

chromatography, HPLC and NMR

Wheaton College, Norton, MA 1991-1993

Research assistant, Biology Department, Supervisor: Dr. Betsey D. Dyer

. Research focused on studying the chemotaxis of termite hindgut

endosymbiont Streblomastix strix. Developed double blind assay for

protist movement and scoring of nutrient sensing

. Laboratory teaching assistant for genetics course and cell biology

course (1992 - 1993)

. Research techniques used include: microscopy, cell biology, molecular

biology (PCR)

Harvard School of Public Health, Boston, MA Summer

1992

Summer intern, Advisor: Dr. Robert Schlegel

. Research focused on studying cell cycle regulation in primary human

foreskin keratinocytes

Research & software techniques

Microscopy, Tissue culture, DNA/RNA purification, PCR, RT-PCR, qPCR,

sequencing, array-based whole genome gene expression and methylation

profiling, genomics analyses and signature discovery, MDS and Perkin Elmer

plate readers for fluorescence and luminescence, Image Xpress, Opera and

ArrayScan high content automated imaging systems, ABi and LightCycler HT

PCR systems, automated plate handling and robotics, Illumina array

profiling and sequencing platforms, NMR, LC/MS, HPLC, chem-informatics

(Accelyrs, Spotfire and GraphPad), bio-informatics and genomics analysis

(GenePattern, GenomeStudio, Ingenuity, Cytoscape), and internal and public

databases of Chemical, Genomic, Genetic and Protein interactions, MS

Office, Adobe suite

Education

Brown University, Providence, RI May

2000

Ph. D. Department of Molecular and Cell Biology and Biochemistry

1994-1999

Thesis: Participation of the Drosophila TGF-?/BMP gene glass bottom

boat/60A in development

Wheaton College, Norton, MA

1991-1993

B. A., Biology, Phi Beta Kappa, Magna cum Laude, GPA of 3.74

Hartford College for Women, Hartford, CT

1989-1991

A. A., GPA of 3.8

Patents

US patent application PCT/61/097,770 "Sonic hedge hog inhibitors" Harvard

reference: 3216

US patent application PCT/US09/41381 "Compositions and methods for

promoting the generation of pdx1+ pancreatic cells" Harvard reference: 3197

US patent application PCT/61/150,509 "Small molecule inducers of definitive

endoderm from mouse and human embryonic stem cells" Harvard reference: 3605

Publications

. Shuibing Chen, Malgorzata Borowiak, Julia L. Fox, Rene Maehr, Kenji

Osafune, Lance Davidow, Kelvin Lam, Lee F. Peng, Stuart L. Schreiber,

Lee L. Rubin, Douglas Melton. 2009. "Small molecules that direct

differentiation of human embryonic stem cells toward the pancreatic

lineage". Nature Chemical Biology, 5(4), 258-65. Patent application

filed.

. Malgorzata Borowiak, Rene Maehr, Shuibing Chen, Alice E. Chen, Weiping

Tang, Julia L. Fox, Stuart L. Schreiber, Douglas A. Melton. 2009.

"Small molecules efficiently direct differentiation of mouse embryonic

stem cells into definitive endoderm". Cell Stem Cell 4(4), 348-58.

Patent application filed.

. Benjamin Z. Stanton, Lee F. Peng, Nicole Maloof, Kazuo Nakai, Xiang

Wang, Kara M. Herlihy, Jay L. Duffner, Kennedy Taveras, Joel M. Hyman,

Sam W. Lee, Angela N. Koehler, James K. Chen, Julia L. Fox, Anna

Mandinova and Stuart L. Schreiber. 2009. "A small molecule that

binds Hedgehog and blocks its signaling in human cells". Nature

Chemical Biology 5(3), 154-156. Patent application filed.

. Richard I. Sherwood, Cristian Jitianu, Ondine Cleaver, David A.

Shaywitz, Julia O. Lamenzo*, Alice E. Chen, Todd R. Golub, Douglas A.

Melton. 2007. "Prospective isolation and global gene expression

analysis of definitive and visceral endoderm". Dev. Biol. 304(2), 541-

55

. Young-kwon Kim, Midori A. Arai, Takayoshi Arai, Julia O. Lamenzo,

Elton F. Dean, III, Nick Patterson, Paul A. Clemons, Stuart L.

Schreiber. 2004. "Relationship of stereochemical and skeletal

diversity of small molecules to cellular measurement space". J. Am.

Chem. Soc., 126, 147**-*****.

. Haerry, T. E., Khalsa, O O'Connor, M. B. and Wharton, K. A. 1998.

"Synergistic signaling by two BMP ligands through the SAX and TKV

receptors controls wing growth and patterning in Drosophila".

Development, 125, 3977-3987.

. Khalsa, O., Yoon, J-W., Torres-Schumann, S. and Wharton, K. A. 1998.

"TGF /BMP superfamily members, Gbb-60A and Dpp, signal in a

synergistic manner to pattern Drosophila appendages". Development,

125, 2723-2734.

. Wu, R., Orji, C. C., Michalcyk, R., Schmidt, J. G., Li, Z., Stocking,

E., Ashburn, D. A., Kelly, J. A., Khalsa, O., Martinez, R. A.,

Mariappan, S. V. S., Unkefer, C. J., and Silks, L. A. 1999. "Synthesis

and application of stable isotope labeled of nucleic acids, amino

acids and carbohydrates". Recent Research Developments in Organic

and Bioorganic Chemistry 3, 1-27.

. Chang, T.; Khalsa, O., Wang, H.; Lee, M. and Schlegel, R. 1996.

"Staurosporine Resistance Accompanies DNA Tumor Virus-induced

Immortalization and Is Independant of the Expression and Activities of

ERK-1, ERK-2, Cyclin A, Cyclin- dependent kinase (cdk) 2 and cdk4".

Cell Growth & Differentiation, 7 (3), 361-372.

. Dyer, B. D. and Khalsa, O. 1993. "Surface bacteria of Streblomastix

strix are sensory symbionts". BioSystems, 31, 169-180.

Presentations

. Julia Lamenzo*. "Chemical Screening for Modulators of b cell

Differentiation and Proliferation". Slide Presentation, Discovery on

Target 2008: Targeting Diabetes with Novel Therapeutics.

. Julia Lamenzo, Calvin Balatbat, Jennifer Hyoje Ryu, Anastasie

Kweudjeu, Jennifer Lock, Susan Bonner-Weir, Gordon Weir and Doug

Melton. "Screening for b cell replication and expansion in vitro".

Poster Presentation, 2008 Investigators Retreat, Beta Cell Biology

Consortium of the NIDDK/NIH.

. Julia Lamenzo, Malgorzata Borowiak, Shubing Chen, Kenji Osafune,

Anastasie Kweudjeu, Lee Peng, Stuart L. Schreiber and Doug Melton.

"Chemical induction of b cell genes." Slide Presentation, 2007

Investigators Retreat, Beta Cell Biology Consortium of the NIDDK/NIH.

. Julia Lamenzo, Calvin Balatbat, Danwei Huangfu, Shubing Chen, Esther

O'Sullivan, Jennifer Lock, Anastasie Kweudjeu, Susan Bonner-Weir,

Gordon Weir, and Doug Melton. 2007. "Screening for b cell replication

and expansion in vitro". Poster Presentation, North East Regional

Meeting of the Society for Developmental Biology.

. Julia Lamenzo, Lee Peng, David Shaywitz, Rene Maehr, Hiram

Chipperfield, Cristian Jitianu, Anastasie Kweudjeu, Jake Zucker,

Kennedy Taveras, Stuart Schreiber, Todd Golub and Doug Melton.

"Screening for Specified Embryonic Stem Cell Differentiation", Slide

Presentation, 2006 North East Regional Meeting of the Society for

Developmental Biology.

. Khalsa, O. **, Yoon, J-W., Torres-Schumann, S. and Wharton, K.A. 1998

"TGF /BMP superfamily members Gbb-60A and Dpp signal in a synergistic

manner to pattern the Drosophila wing". Slide Presentation, Society

for Developmental Biology, North-East Regional Conference.

. Khalsa, O., and Wharton, K.A. 1998. "Gbb-60A signaling in the wing

negatively regulates ci". Slide Presentation, Annual Drosophila

Research Conference.

. Haerry, T.E., Khalsa, O., Wharton, K.A., and O'Connor, M.B. 1998.

"Synergistic threshold signaling by two BMP ligands through the SAX

and TKV receptors controls wing growth and patterning in Drosophila".

Slide Presentation, Annual Drosophila Research Conference.

*name changed: November, 2008, due to marriage from J. O. Lamenzo to J. L.

Fox

**name changed: January, 2000 in Massachusetts State Court from O. Khalsa

to J. O. Lamenzo

Honors and Awards

Harriet V. Davis Award in science, Hartford College for Women,

1990

Chidsey-Marshall Scholarship in science, Wheaton College, 1992

NIH training grant, Brown University, Spring 1996,

1997, 1998

Co-Investigator, Beta Cell Biology Consortium, NIDDK/NIH

2006-2009

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