HIstotech/Scientist

Jorge Solares

**** ** *** ******

Miami, Florida 33176

************@*******.***

Mobile: 305-***-****

EDUCATION

University of Aberdeen 2001-2002 Aberdeen, UK

MSc, Medical Molecular Genetics

Completed under supervision of Dr. Matthew Wright

Subject: Characterization of the ratp28 low affinity glucocorticoid receptor variant known as HC5

Other techniques employed include: RNA/DNA purification, viral titering, RT-PCR, PCR, Northern Blotting,

Western Blotting, oligo labeling with P32, and cloning

Elon University, 1995-1999 Elon, NC

BS Biology, Minor Chemistry

CERTIFICATIONS

-Licensed Histotechnologist for the state of Florida, license number: TN 45648 expires 8/31/2014

- QIHC (ASCP) certified, expires 7/2/2015

-Currently training in cGMP and GLP practices

EXPERIENCE

Biomedical Research Technician: Cherokee Nation Business, October 2012-August 2013 San Antonio, Texas

40 hours/week, Brooke Army Medical Center, Institute of Surgical Research

-Engaged in studying bufodienolide concentrations in plasma, urine, adrenals and other tissues from rats subjected to battlefield trauma.

-Responsible for purifying bufodienolide from rat blood, adrenals, and brain using basic chemical separation methods and Waters C18/HLB columns. Also responsible for determining the quality and quantity of bufodienolide by HPLC (Beckman Gold) and mass spectrometry (Agilent)

-Other responsibilities include updating and writing SOP's, developing new assays, keeping day to day logs, teaching high school students, and attending meetings

IHC Senior Technologist: LabCorp Integrated Genetics, 2006- July 2012 New York, NY

6 years, 40 hours/ week

- Responsible for performing diagnostic IHC manually and using Bond Max, Ventana, and Dako auto stainers. Also responsible for fixing tissue, microtomy, embedding, preparing solutions, and performing special stains such as Congo Red, AFB, and Mucicarmine

- Involved in protocol design and trouble shooting for many different IHC stains on different tissues. Trouble shooting also encompassed issues with fixation, microtomy, and any technical or mechanical errors that might arise

- Quality control, screening and scoring for ER, PR, Her2, p53 and other cancer markers. Other responsibilities with screening cases included writing and editing protocols, control selection, and antibody titering and optimization

- Involved in performing and screening FISH for Her2/Neu for prognostic/ therapeutic cases, and approving stain only cases for outside clients

-Responsible for assay validation and compliance for New York state and FDA standards.

Research Assistant, Soft Tissue Laboratory at Hospital for Special Surgery, 2003-2006 New York, NY

3 years, 40 hours/week

- Involved in studying the effects of age on fracture healing in mice and positional cloning chemically induced mutations in mice

- Responsible for the day to day activities of Dr. Aaron Daluiski’s lab including ordering supplies maintaining mouse colonies, keeping lab records (data management, statistics), writing protocols and IUCAC applications, training summer students, giving research presentations (poster design and power point), and writing reports for publication

- Techniques used in the lab included in situ hybridization using S35 and Dig labeled RNA probes, RNA/DNA purification (Triazole, Qiagen MIDI kits, phenol/chloroform), cloning and subcloning, positional cloning (genotyping and primer design), PCR strategies, RT PCR (SYBR green from Bio Rad), immunohistochemistry (Antibody optimization), Brdu labeling, microscopy (photoshop), micro CT imaging, mouse breeding, cell culture, mouse femoral surgery (sterilization of instruments, necropsies), basic histology (fixation, microtomy, staining), histomorphometry, embryo harvesting (timed pregnancies) and full mount skeletal preps

Research Assistant II, Department of Epidemiology at The University of Miami, 2002-2003 Miami, FL

1 year, 40 hours/week

- Responsible for quantifying the presence of small quantities of HIV gag, rRNA, and proviral DNA

in drug paraphernalia using taq man real-time PCR (Applied Biosystems).

- Utilized techniques including RNA/DNA purification, T7 transcription, cloning, MIDI/MINI plasmid (Qiagen)

prep and cell culture

TECHNICAL SKILLS

Haplotype analysis, lambda phage purification, SDS/PAGE, bacterial cloning, electrophoresis, ELISA,

cell culture, protein purification, in situ hybridization, HPLC, and bioinformatics (primer/probe design,

sequence alignment).

PUBLICATIONS

Leel V, Elrick LJ, Solares J, Ingram N, Charlton KA, Porter AJ, Wright MC.

Identification of a truncated ratp28-related protein expressed in kidney.

Biochem Biophys Res Commun. 2004 Apr 9;316(3):872-7.

Khan SN, Solares J, Ramsey KE, Yang X, Bostrom MP, Stephan D, Daluiski A.

Identification of novel gene expression in healing fracture callus tissue by DNA microarray.

HSS J. 2008 Sep;4(2):149-60. Epub 2008 Aug 28

PERSONAL

Languages: Fluent in Spanish

REFERENCES

Brooke Army Medical Base, Institute of Surgical Research San Antonio, TX

Supervisor: Dr. Daniel Darlington

Tel: 410-***-****

Contract Supervisor: Dawn Munoz

Tel: 210-***-****

Email: ****.*****@**-***.***

LabCorp Integrated Genetics New York, NY

Supervisor: Stepahie Zabski

Tel: 212-***-****

Email: *******@*******.***

Manager: Valerie Joseph

Tel: 212-***-****

Email: ******@*******.***

Director of Technical Services: Mary Ann Gangi

Tel: 917-***-****

Email: ******@*******.***

Director of Anatomical Pathology: Evelyn Vazquez

Tel: 212-***-****

Email: *******@*******.***

Hospital for Special Surgery New York, NY

Assistant Attending/ Assistant Professor, Weill Medical College of Cornell University

Surgeon, Hand & Upper Extremity

Dr. Aaron Daluiski, MD

Tel: 212-***-****

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