Assistant Project

Ankit V. Talesara

347-***-**** *****.********@*****.***

*** ******** ******, ********* *, East Boston, MA 02128

EDUCATION

Northeastern University, Boston, MA Graduated 2013

MS in Biotechnology (Molecular Track)

GPA – 3.47/4.00

Visvesvaraya Technological University, Belgaum, India July 2010

Bachelor of Engineering in Biotechnology

GPA – 3.95/4.0

LAB SKILLS

Reverse Line Blotting Nanodrop Spectrophotometer KOVA colony counting

Miniblotter 45 (Immunetics) UV/Vis GLP/GxLP/CLIA Expression Cloning

qPCR/RT-PCR Blood Spiking HPLCs/ Chromatography French Press

5’-RACE

Primer Designing Affinity Purification IEC

3’-RACE SEC

QIAcube Automated system Bacterial/Fungal Culturing

Protein Purification Protein Expression ELISA DNA/RNA Purification

Immunoprecipitation Molecular Cloning GC-MS

X-ray Autoradiography

SDS PAGE Bacterial Transformation cDNA Preparation Next Gen sequencing

Western Blotting Alphaimager Gel Electrophoresis FACS

EXPERIENCE

June 2013 – Present

- Immunetics Inc, Boston, MA

Research Associate (Molecular Diagnostics and Clinical Validation – Endocarditis and Fungal Project)

Main goal is to develop immune assay diagnostic kit for detection and Validation of Infectious endocarditis and Antibiotic

resistant bacteria using DNA microarray based Reverse Line blot (RLB) technique.

Obtain rRNA sequence using ARB/SILVA. Design specific probes and primers for specific bacterial strains.

Extract DNA from live patient blood samples using QIAcube Automated system. Carry out PCR using the designed Primers.

Make Blots using the patented technology (Immunetics) and hybridise them with the samples and probes using the patented

CodaXcel instrument designed by Immunetics.

Analyse the Blots and optimize the experiment to increase the specificity and sensitivity of the RLB Diagnostic Kit .

Repeated the same methodology for MRSA (Methicillin Resistant S.aureus) and fungal pathogens.

February 2012 – September 2012

- Massachusetts General Hospital, Blower Laboratory, Boston, MA

Research Assistant (Department of Molecular Biology)

Isolate various genes of interest using RT -PCR and priming techniques for gene silencing studies

Clone and transfect the genes in E.coli and BL21 cells for screening and Protein expression

Perform Western Blot to analyze levels of protein expression at different time points and extract the proteins

Use the extracted proteins to separate raised antibodies from the chicken serum

February 2012 – May 2012

- Antimicrobial Discovery Centre (Dr. Kim Lewis Lab), Northeastern University, Boston, MA

Research Assistant (Unculturable Group)

Grew and screened microbes from samples of sewage water treatment plant

Plated pure cultures using dependent colonies

Regrew the colonies in close proximity under varying conditions and develop an optimized repr oducible pathway in

anaerobic conditions

January 2010 – May 2010

- Gandhi Krishi Vignan Kendra, University of Agricultural Sciences, Bangalore, India

Lab assistant (Department of Agricultural Microbiology)

Isolated and characterized various Microbes using silver staining and Gram Staining techniques

Selected Suitable medias, Substrates (Cow dung, Whey, Sewage water), Mediators, Nutrients & Electrodes and Constructed

various types of Microbial Fuel Cells (Organic Bagasse cell, Aquatic Sedimentation cell, Algal Hydrogen Cell) which

successfully generated workable volume of electricity

PUBLICATION

January 2013 – April 2013

- Chromosome Cancer Gene Project, Analytical Biochemistry, Northeastern University

The aim of this project is to map cancer -associated genes on each chromosome by integrating information from various web

sites (primarily Sanger, Waldman and GeneCards, and Cancerindex) and construct a complete list of the degrees of cancer

association of the oncogenes.

Publication is pending.

COMPUTER SKILLS

ARB, SILVA, Sequencher, M-baser, Visual Basic, Blast, ClustalW, Expasy, PDB, Uniprot and Genome Database, NCBI, SQL, DBMS,

MS Office.

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