CHAO CHEN

Address: Institute of Vascular Medicine, Peking University Third Hospital, Beijing 100191, China

Mobile +(01-404-***-****

E-mail: ab91ho@r.postjobfree.com

Education

**** **** ****** **IVERSITY, BEIJING, CHINA

Ph.D. degree in Pathophysiology, Supervisors: Dr. Youyi Zhang.

2002 2007 JILIN UNIVERSITY, CHANGCHUN, CHINA

M.D. Clinical Medicine GPA: 4.3 Rank: 4th/36.

Research Experience

2007 – 2012 As a Ph.D student

Project I: -AR-induced IL-6 via cAMP/Epac/PKC /P38 pathway

Our lab has done a lot of work on linking the sympathetic activation to cardiac inflammation during chronic heart failure

and has previously reported that β-adrenergic receptor (β-AR) induced IL-6 production in neonatal mouse cardiac

fibroblasts through Gs/cAMP/p38 pathway but independent of protein kinase A (PKA). My project focus on finding the

missing link between cAMP and p38 signaling pathways in cardiac fibroblasts. By using various protein kinase

inhibitors and adenovirus-mediated shRNAs to interfere with key signaling molecules, I reported β-AR activated

cAMP/Epac/PKC /p38 pathway to produce IL-6, and firstly demonstrated PKC is a novel downstream effector of

β-AR/cAMP/Epac pathway. (The paper has been accepted to British Journal of Pharmacology)

Project II: Gab1 (Grb-2-associated binder 1) protects the mouse heart against ischemia/reperfusion (I/R) injury

The project collaborates with Prof. Jincai Luo (Laboratory of Vascular Biology, Institute of Molecular Medicine, Peking

University, China), who generated the cardiac-specific Gab1 knockout mice. I independently do all the experiments to

monitor the progression of ischemia reperfusion injury of wild-type and Gab1 KO mice by echocardiography (Vevo 770TM),

hemodynamic measurements (via carotid using Millar catheter), 2,3,5-triphenyltetrazolium chloride staining after 30 min of

ischemia, 2 h or 24 h reperfusion. And I also involved in the isolation and culture of adult mouse cardiomyocytes. My

results show Gab1 deficiency dramatically increases cardiac I/R injury, which firstly present the critical role of Gab1 in I/R

injury. (All the data are summarized and submitted to Basic Research in Cardiology).

Besides, I’m involved in most research projects in our lab (see my publications list).

Publications

1. Chen C, Du J, Feng W, Song Y, Lu Z, Xu M, Li Z, Zhang Y. -adrenergic receptors stimulate interleukin-6 production

through Epac-dependent activation of PKCδ/p38 MAPK signaling in neonatal mouse cardiac fibroblasts. Br J Pharmacol.

2012 May;166(2):676-88. [PMID: 22103274]

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2. Sun L *, Chen C *, Jiang B, Li Y, Yang X, Yang Y, Zhang R, Lu Y, Zhu D, Huo Y, Feng G, Zhang Y, Luo

J.Grb2-associated binder 1 is essential for cardioprotection against ischemia/reperfusion injury. Basic Res Cardiol. [under

revision, co-first author]

3. Ma X, Song Y, Chen C, Fu Y, Shen Q, Li Z, Zhang Y. Distinct actions of intermittent and sustained β-adrenoceptor

stimulation on cardiac remodeling. Sci China Life Sci. 2011 Jun;54(6):493-501. [PMID: 21706409]

4. Feng W, Song Y, Chen C, Lu ZZ, Zhang Y. Stimulation of adenosine A(2B) receptors induces interleukin-6 secretion in

cardiac fibroblasts via the PKC-delta-P38 signaling pathway. Br J Pharmacol. 2010 Apr;159(8):1598-607. [PMID:

20050850]

5. Li Y, Zhang H, Liao W, Song Y, Ma X, Chen C, Lu Z, Li Z, Zhang Y. Transactivated EGFR mediates

{alpha}1-AR-induced STAT3 activation and cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2011

Nov;301(5):H1941-51 [PMID: 21856923]

6. Li H, Lu ZZ, Chen C, Song Y, Xiao H and Zhang Y. High-resolution echocardiographic assessment of -adrenergic

stimulation-induced heart failure with reduced heart rate in mice. Clin Exp Pharmacol Physiol. [under revision]

7. Li F, Shao Y, He J, Qin X, Chen C, Wang Y, Sun L, Li Y, Zhao X. Construction of complete hERβ eukaryotic expression

vector and its expression in PC-3M cell line. Journal of Jilin University Medical Edition. 2006, 32(2) 189-193

Conference Presentations

1.Chen C, Du J, Feng W, Song Y, Lu Z, Xu M, Li Z, Zhang Y. A Novel cAMP/epac/pkcδ/p38 Pathway Controls

β-AR-induced Il-6 Production In Neonatal Mouse Cardiac Fibroblasts. American Heart Association's (AHA) Committee on

Scientific Sessions, Orlando, Florida, November 2011. (Poster)

2.Chen C, Zhang Y. -adrenergic receptors stimulate interleukin-6 production through Epac-dependent activation of

PKCδ/p38 MAPK signaling in neonatal mouse cardiac fibroblasts. XI Chinese Association of Pathophysiology, Nanjing,

China, November 2010. (Oral Presentation, Award the Second Place in the Session of Youth Competitions Excellent Papers)

3.Chen C, Du J, Feng W, Song Y, Lu Z, Xu M, Li Z, Zhang Y. A Novel Pathway Controls β-AR-induced Il-6 Production In

Neonatal Mouse Cardiac Fibroblasts. Chinese Physiology receptors, cancer and immune Joint Committee of Conference

Proceedings,Hefei,China, April 2010.(Poster)

4.Li H, Chen C, Zhang Y. High-resolution echocardiographic assessment of -adrenergic stimulation-induced heart failure.

XI Congress of International Society for Heart Research Chinese Section, Guangzhou, China, Nov 2012. (Poster)

5. Liu X, Gai Y, Liu F, Chen C, Xu M, Li Z. Trimetazidine inhibits pressure overload-induced cardiac fibrosis in rats.

The 7thScientific Conference on Cardiovascular Sciences Across the Strait, Kunming, China, August 2009. (Poster)

Experimental Skills

Animal Models: Rat&Mice Cardiac Hypertrophy and Myocardial Injury

Cardiac Hypertrophy: TAC (Transverse aortic constriction), Adrenergic Receptor agonist /Angiotensin II stimulation (by

using osmotic pump or subcutaneous injection) and Swimming or Running induced physical hypertrophy.

Myocardial Injury: Ischemia (Permanent left coronary artery ligation), Ischemia/Reperfusion (Reperfusion after temporary

left coronary artery ligation)

Heart Function Evaluation: Electrocardiogram, Blood Pressure, Hemodynamic Measurements (Millar catheter or

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Scisience catheter), Echocardiographic Evaluation (Vevo 770 High Resolution Ultrasound)

Molecular and cell biological skills: Western blot analysis, Real-time PCR, ELISA, Co-IP assay, Kinase Activity analysis,

Pull-down assay, Collagen synthesis determined by measuring incorporation of 3H-proline, Protein synthesis in

Cardiomyocytes by 3H-leucine incorporation, Membrane proteins and nuclear protein separation, Plasmid construction and

transfection, Adenovirus construction and infection.

Cell culture: cell lines (H9C2, HEK 293, NIH/3T3), Primary cell isolation and culture: Neonatal Cardiomyocytes and

Fibroblast, Adult Cardiomyocytes and Fibroblast.

Morphological skills: H&E, Immunohistochemistry (including frozen medium embedded and tissue slice preparation),

Confocal immunofluorescence.

Honors and Rewards

The Bachelor Scholarship of Jilin University (2002 ~ 2007)

The Ph.D Scholarship of Peking University (2007 ~ 2012)

Jilin University Excellent Student (2002 ~ 2007)

The Second Place in the Session of Youth Competitions Excellent Papers (XI Chinese Association of Pathophysiology,

Nanjing, 2011)

Teaching Experience

Teaching Assistant for Echocardiography of Small Animals

Research Assistant for Experimental Course in Molecular Pharmacology

Referrals

Youyi Zhang

M.D., Ph.D., Professor (My mentor), Director

Institute of Vascular Medicine, Peking University Third Hospital, Peking University, Beijing, China

Tel: 86-10-828*-****

Email: ab91ho@r.postjobfree.com

Jincai Luo

M.D., Ph.D., Professor, Acting Principal Investigator

Laboratory of Vascular Biology, Institute of Molecular Medicine, Peking University,Beijing, China

Tel: 86-10-627*-****

Email: ab91ho@r.postjobfree.com

Baoyue Yang

M.D. Ph.D, Professor, Vice Chair

Department of Pharmacology, Peking University,Beijing,China

Phone: 86-10-828*-****

E-mail: ab91ho@r.postjobfree.com

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