Assistant Manager
Resume:
George D. Glekas, Ph. D.
Work Address: Home Address:
*** *. ******* ******, *** MSB 901 E. Colorado Ave. #75
Urbana, IL 61801 Urbana, IL 61801
*******@*****.***
OBJECTIVE
To obtain a research or research and development position that utilizes my biochemical and problem-solving skills
in a pharmaceutical, crop science or biotechnology field.
EDUCATION
UNIVERSITY OF ILLINOIS Urbana, IL
Ph.D. in Biochemistry Dec. 2008
UNIVERSITY OF NORTH CAROLINA Chapel Hill, NC
B.S. in Chemistry (Minor in music) May 1997
ACADEMIC AND PROFESSIONAL EXPERIENCE
UNIVERSITY OF ILLINOIS Urbana, IL
Jan. 2009 – Present
Postdoctoral Research Associate
Worked in the laboratory of Dr. George Ordal on numerous projects to elucidate the chemotaxis
pathway in Bacillus subtilis, with a focus on membrane-bound chemotaxis receptors and post-
translational modification s
UNIVERSITY OF ILLINOIS Urbana, IL
Aug. 2000 – Dec. 2008
Graduate Research Assistant
Doctoral thesis work in the labor atory of Dr. George Ordal on various aspects of signal transduction in
the chemotaxis system of B. subtilis
BASF CORPORATION RTP, NC
Aug. 1997 – Aug. 2000
Assistant Biologist/Greenhouse Manager
Planned, conducted, and cooperated in laboratory, greenhouse, and field experiments to determine
fungicide efficacy and sensitivity
UNIVERSITY OF NORTH CAROLINA Chapel Hill, NC
Jun. 1996 – Aug. 1997
Undergraduate Research Assistant
Worked in the laboratory of Dr. Roger Miller with nitric acid, sulfuric acid and ammonia aer osols and
probed their effect on the atmosphere using spectrometry
UNIVERSITY OF NORTH CAROLINA Chapel Hill, NC
Aug. 1993 – Oct. 1996
Research Assistant
Studied heart rate variability and its effects on myocardial ischemia, both silent and painful, under the
supervision of Dr. David Sheps
SKILLS AND TRAINING
Chromatography: FPLC and HPLC (reverse-phase, ion exchange, affinity, and gel filtration)
Crop Science: Fungicide, herbicide and insecticide spray tests, fungal pathogen isolation, plant growth
Immunology: Antibody purification and Western analysis
Methylation Assays, including with radioactivity
Microbiology: Numerous in vivo biochemical assays in prokaryotic systems
Microscopy: Fluorescence and Phase Contrast, live cell tracking
George D. Glekas – 2
Molecular biology: Bacterial chromosomal and plasmid DNA isolation, bacterial phage transduction,
bacterial transformation, PCR, cloning and mutagenesis
Molecular Modeling: Phyre, LOMETS, I-TASSER, Modeler, Rosetta, PyMOL, VMD
Phosphorylation Assays, including with radioactivity
Protein Characterization and Interactions: Isothermal Titration Calorimetry (ITC), Pulldown Assays,
Surface Plasmon Resonance (SPR), Circular Dichroism (CD)
Protein Purification: Soluble and membrane-bound
Short courses: Genomics, Proteomics and Bioinformatics (University of Illinois), Flow cytometry
(University of Illinois), and Isothermal Titration Calorimetry (University of Illinois)
Computer: Multiple software packages including Adobe Photoshop and Dreamweaver, Corel Graphics
Suite, DOS, Linux, Matlab, Microsoft Office and numerous structural modeling and alignment packages;
Network set-up; Hardware installation; Web site design
Illinois Certificate in Business Administration for Scientists (University of Illinois)
Fluent in English and Greek, proficient in French
SELECTED PUBLICATIONS
(12 total publications)
Yuan W*, Glekas GD*, Allen GM, Walukiewicz HE, Rao CV and Ordal GW. (2012) The importance of the
interaction of CheD with CheC and the chemoreceptors compared to its enzymatic activity during chemotaxis in
Bacillus subtilis. PLoS One. 7:e50689. *Co-first authors
Glekas GD, Mulhern BJ, Kr oc A, Duelfer KA, Lei V, Rao CV and Ordal GW. (2012) The Bacillus subtilis
chemoreceptor McpC senses multiple ligands using two discrete mechanisms. J. Biol. Chem. 287:39412-8.
Glekas GD*, Plutz MJ*, Walukiewicz HE*, Allen GM, Rao CV and Ordal GW. (2012) Elucidation of the multiple
roles of CheD in Bacillus subtilis chemotaxis. Mol. Microbiol. 86:743-56. *Co-first authors
Cannistraro VJ*, Glekas GD*, Rao CV and Ordal GW. (2011) Cellular stoichiometry of the chemotaxis proteins in
Bacillus subtilis. J. Bacteriol. 193:3220-7. *Co-first authors
Glekas GD, Cates JR, Cohen TM, Rao CV and Ordal GW. (2011) Site-specific methylation in Bacillus subtilis
chemotaxis: effect of covalent modifications to the chemotaxis receptor McpB. Microbiology 157:56-65.
Glekas GD, Foster RM, Cates JR, Estrella JA, Wawrzyniak MJ, Rao CV and Ordal GW. (2010) A PAS domain
binds asparagine in the chemotaxis receptor McpB in Bacillus subtilis. J. Biol. Chem. 285:1870-8.
MENTORING AND TEACHING EXPERIENCE
UNIVERSITY OF ILLINOIS Urbana, IL
Jan. 2004 – Present
Mentor for Undergraduate Research Students
Supervised undergraduate research for seven senior thesis students and 12 undergraduate research
assistants
UNIVERSITY OF ILLINOIS Urbana, IL
Jan. 2001 – Dec. 2001
Teaching Assistant
Jan. 2006 – May 2006
Instructed undergraduate general chemistr y laboratory classes (CHEM 106)
Led discussion group-based class for undergraduate cell biology classes (MCB 252)
AWARDS
University of Illinois Teaching Award - “Teachers Ranked as Excellent” – Rated as Outstanding (highest
rating), Spring 2001, Fall 2001, Spring 2006
IBM Watson Scholarship, 1993-1997
National Merit Scholar, 1993-1997
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