Engineering Project

SANDEEP KUMAR

Correspondence Address:

Department of Biotechnology

National Institute of Technology (NIT)

Jalandhar, Punjab 144011

Phone No: 092********

E-mail: ********.**.***@****.**.**,

*************@*****.***

Residential Address:

Sandeep Kumar

S/O Sh Gopi Ram

Behind Chungi,

Dadri Gate, Bhiwani,

Haryana, India -127021

Career Objective

To work with challanges and new directions with implimentation of all the knowledge and skills

gained in life with simultaneous learning of associating aspects, along with humanity and ethics of life

and profession.

Ph. D. Research Project

Immobilization of Enzyme Serratiopeptidase on Magnetic Nanoparticles (MNPs) and

Characterization of Resulted Nanoparticles

Major Research Objectives

Preparation of magnetic nanoparticles

Surface modifications and functionalization of magnetic nanoparticles (MNPs) for its stability

and ease of enzyme attachment

Attachment of enzyme (serratiopeptidase)

Properties studies of enzyme magnetic nanoparticles (EMNPs)

Research supervisor: Dr A. K. Jana,

Professor and Head,

Department of Biotechnology, NITJ

email: ******@****.**.**

M.Tech. Research Project

Preparation of Cross Linked Enzyme Aggregate (CLEA) for enantioselective Nitrile hydrolysis

Major Research Objectives

Purification of enzyme (Nitrilase)

Screening of aggregating and cross linking agent

Optimization of various cross linking and stability parameters

Research supervisor: Dr. U. C. Banerjee,

Professor and Head,

Department of Pharmaceutical Technology (Biotechnology),

NIPER, Mohali

email: **********@*****.**.**

Area of interest

Biopharmaceuticals and Therapeutic Enzymes

Targeted Delivery System

Protein Engineering

Protein and Enzyme Technology

Enzyme Kinetics

Biocatalysis

Protein Purification

Analysis

Intellectual Property Rights

Paper publication

Sandeep Kumar, Asim K. Jana*, Isha Dhamija, Yashpaul Singla, “Direct attachment of

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serratiopeptidase on magnetic nanoparticles for magnetically targetable preparation ” manuscript

under preparation to target Biomacromolecules.

Sandeep Kumar, Asim K. Jana*, Isha Dhamija, Yashpaul Singla, “Chitosan assisted

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immobilization of serratiopeptidase on magnetic nanoparticles, characterization and its targeted

delivery in vitro and in vivo” manuscript ready for Journal of drug targeting.

Sandeep Kumar, Asim K. Jana*, Isha Dhamija, Yashpaul Singla, “Preparation,

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characterization and targeted delivery of serratiopeptidase immobilized on amino-functionalized

magnetic nanoparticles” (Minor revisions in European Journal of Pharmaceutics and

Biopharmaceutics) (IF-4.4)

Kapish Gupta, Asim K. Jana, Sandeep Kumar, Mithu Maiti, “Immobilization of α-amylase

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and amyloglucosidase onto ion-exchange resin beads and hydrolysis of natural starch at high

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concentration”, (2013), Bioprocess and Biosystems Engineering, DOI 10.1007/s00449-013-0946-y.

(IF1.8)

Neeraj Kumar, Shishu, Sandeep Kumar, Asim Kumar Jana, “The Effect of N-Alkyl

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Substituents on the Usability of Imidazolium Cation based Ionic

Liquids in Microemulsion Systems: A Technical Note”, (2013), AAPS PharmSciTech, DOI:

10.1208/s12249-013-9939-z. (IF-1.43)

Neeraj Kumar, Shishu, Gulshan Bansal, Sandeep Kumar, Asim Kumar Jana, “Ditosylate

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Salt of Itraconazole and Dissolution Enhancement Using Cyclodextrins.”,(2012), AAPS

PharmSciTech, 13 (3), 863-874. (IF-1.43)

Neeraj Kumar, Shishu, Gulshan Bansal, Sandeep Kumar, Asim Kumar Jana, “Preparation

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and cyclodextrin assisted dissolution rate enhancement of itraconazolium dinitrate salt.”, (2012),

Drug Development and Industrial Pharmacy, 1-10, DOI: 10.3109/03639045.2012.681382. (IF-1.49)

Sandip V. Pawar, Vachan Singh Meena, Shubhangi Kaushik, Ashwini Kamble, Sandeep

8.

Kumar, Yusuf Chisti, U. C. Banerjee, “Production of nitrilase by recombinant Escherichia coli in a

laboratory scale reactor and its utilization in the enantioselective hydrolysis of mandelonitrile to R- - mandelic acid.” (2012), 3 Biotech, 2 (4), 319-326.

Sandeep Kumar, Utpal Mohan, Ashwini L. Kamble, Sandip Pawar, Uttam C. Banerjee,

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“Cross-linked enzyme aggregates of recombinant Pseudomonas putida nitrilase for enantioselective

nitrile hydrolysis”, (2010), Bioresource Technology, 101(17), 6856-58. (IF- 4.5, Citations: 5)

Conferences

International conference

Abstract in Proceedings and oral presentation: Sandeep Kumar, Asim K Jana, Mithu Maiti

1.

“Immobilization of therapeutic enzyme drug on iron oxide magnetic nanoparticles for targeted

delivery”, International Conference on Sustainable Technologies for Energy and Environment in

Process Industries and Indo-US Joint International Conference on Energy and Environment

(CHEMCON-2012), held on 27-30 December, 2012, NIT Jalandhar (paper id 1018).

2. Full paper in Proceeding and oral presentation: Sandeep Kumar, Asim K Jana,

“MAGNETIC NANOPARTICLES IN ENZYME AND PROTEIN TECHNOLOGY: AN UPDATED

OUTLOOK”, 2nd International Conference on Biomedical Engineering and Assistive

Technologies (BEATS-2012), held on 6-7 December, 2012, NIT Jalandhar, 724-730

3. Full paper in Proceeding: Madan Lal Verma, Sandeep Kumar, Aruna Devi, Asim K Jana,

“Engineering Enzyme for Efficient Biocatalysis”, 2nd International Conference on Biomedical

Engineering and Assistive Technologies (BEATS-2012), held on 6-7 December, 2012, NIT

Jalandhar, 742-746

4. Full paper in Proceeding: Madan Lal Verma, Sandeep Kumar, Aruna Devi, Asim K Jana,

“Strategy to Optimize the Recombinant Gene Expression in Escherichia coli ”, 2nd International

Conference on Biomedical Engineering and Assistive Technologies (BEATS-2012), held on 6-7

December, 2012, NIT Jalandhar, 747-750

5. Full paper in Proceeding and oral presentation: Sandeep Kumar, Asim K Jana, “Enzyme

nanoparticles (ENPs) based biosensors”, International Conference on Biomedical Engineering

and Assistive Technologies (BEATS-2010), held on 17-19 December, 2010, NIT Jalandhar,

paper number: J-315

National Conference

1. Presented a poster on “In vitro Free Radical Scavenging and Antioxidant activity of Moringa

oliefera pods” in APTI 12th Annual National Convention held on 25-27 October, 2007,

University Institute of Pharmaceutical Sciences, Punjab University Chandigarh.

Seminars

“PHRARMACIST HARBORING MANAGERIAL ACUMEN”, held on 23 December 2008,

Institute of Pharmaceutical Science, Kurukshetra University, Kurukshetra.

Academic Career

Year Qualifying Board/University/Institute Marks/ CGPA

Examination

2009- Ph. D. Dr. B R Ambedkar, National Institute of Persuing

current Technology (NIT), Jalandhar

2006-08 M. Tech. National Institute of Pharmaceutical 9.00 CGPA on a 10-

(Pharm.) Education & Research (NIPER), SAS point scale

Nagar, Punjab

2002- 06 B. Pharm Department of Pharmaceutical Sciences, 63%

GJU Hissar, Haryana

Scholarships

Awarded with a fellowship by Department of Biotechnology (DBT), Government of India

for a period of two years (2006-08).

Awarded with a fellowship by Ministry of Human Resource Development (MHRD),

Government of India for a period of four years (2009-2012).

Technical Skills

Instruments Handled

Experience in handling of HPLC, GC, FPLC, UV-Visible spectrophotometer, Sonicator,

French press, Dyno mill, Probe sonicator, Centrifuge, Microscope, Packed bed reactor, Stirred

and continuous bioreactor.

Biochemical Methods & Recombinant DNA Technology

Experienced hand in Gel electrophoresis techniques (SDS PAGE, Native PAGE, Agarose),

Western Blotting, Chromatographic methods- affinity, ion exchange, size exclusion. Plasmid

isolation technique, competent cell preparation, PCR.

Microbiological Technique

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Well versed in microbial cell culture techniques, aseptic handling and sterilization methods,

preparation and inoculation of different culture media, media optimization, screening of

microorganism, enzyme and cell immobilization using different matrices.

Pharmacological Techniques

Trained in handling of animals, administrations of drugs through various routes like IV, IM, IP

and oral.

Additional Qualification

Qualified GATE organized by IIT Kharagpur, 2006, with rank 611

Completed distance learning “General Course on Intellectual Properties” from Feb 1 to March

15, 2007, conducted by World Intellectual Property Organization

Computer Proficiency

Windows operating systems and application software: MS Office, ChemDraw, Reference

Management Software (Endnote Plus), Sigma Stat, Sigma Plot and Adobe Photoshop. Scientific data

retrieval from various Internet portals like Sciencedirect, Pubmed, Google, Highwire, Scirus, Scopus

etc.

Interest and Hobbies

Reading novels & books

Desire to learn music

Sports (cricket, volleyball, table tannis etc)

Spiritual practices (dhayan, pranayam)

REFERENCES

Dr. A. K. Jana

Professor and Head

Department of Biotechnology

National Institute Technology (NIT)

Jalandhar, Punjab-144011

Email: ******@*****.**.**

Dr. U. C. Banerjee

Prof. and Head, Department of Pharmaceutical Technology

National Institute of Pharmaceutical Education and Research (NIPER)

Mohali, Punjab- 160062

Email: **********@*****.**.**

Dr. Milind Parle

Professor

Department of Pharmaceutical Sciences

Guru Jambheshwar University of Science and Technology

Hisar, Haryana-125001

Email : ******@**********.***

Dr. Dinesh Dhingra

Associate Professor,

Department of Pharmaceutical Sciences

Guru Jambheshwar University of Science and Technology

Hisar, Haryana-125001

Email : ***********@**********.***

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