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Ph.D. in Pharmacology

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Country: United States
State: New York
City: New York
ZIP: 10001
Salary: 80,000
Posted date: 4/30/2012   all resumes
Email: zzt2s8@r.postjobfree.com
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AREA OF EXPERTISE

• Pharmacology
• G protein coupled receptor signal transduction pathway

PROFESSIONAL SKILLS

Electrophysiology:
• Intranuclear micro-injection, whole cell patch-clamp.
Molecular biology:
• DNA isolation and assay (plasmids amplification and isolation), PCR, plasmids construction (cloning and sub-cloning), site-directed mutagenesis, DNA sequencing and analysis.
• RNA isolation and assay (RNA isolation from tissue and cells, RT-PCR).
• Yeast two-hybrid screening: cDNA library construction, conventional yeast two-hybrid screening (baits construction, yeast growth assay by mating or co-transformation, β-galactosidase activity assays in liquid culture, yeast plasmids extraction), DUAL-membrane system yeast two-hybrid screening.
• Protein extraction and assay (protein extraction from cultured cells, protein assay, western immunoblotting analysis, immunoprecipitation and co-immunoprecipitation).
• Single cell RT-PCR.
• qPCR analysis.
• Gene knockdown (siRNA).
Imaging:
• Confocal microscopy, inverted-fluorescence microscopy, phase-contrast microscopy.
Cell biology:
• Retroviral generation of stable cell lines.
• Immunocytochemistry.
• mammalian cell culture (both primary and cell line), transfection.
• Luciferase assay and dual-luciferase assay.
• Cell proliferation assay, migration and invasion assay.
Animal handling:
• Dissection and micro-dissection; acute cell isolation (rat superior cervical ganglion and retinal bipolar cells).
• Nude mice tumor zenograft (subcutaneous injection and subrenal capsule injection); tumor measurement; tumor isolation, sample process and handling.

RESEARCH EXPERIENCE

New York University New York, NY
Postdoctoral Fellow 2010 to 2011
• Characterize structural and functional domains of p44 in its interaction with androgen receptor and Smad1 in prostate cancer.
• Effects of p44 on androgen regulated genes in androgen dependent cell line.
• Generate mouse tumor xenograft model using both androgen-dependent and –independent cell lines.
• Generate retroviral stable cell lines.

Kent State University Kent, OH
Ph.D. 2003-2009
• Developed acute isolation and cell culture protocol of primary rat retinal bipolar cells.
• Constructed rat retinal cDNA library.
• Characterized G protein coupling profile of metabotropic glutamate receptor 6 using reconstitution system and whole cell patch-clamp technique.
• Identified endogenous coupling G protein of mGluR6 in rat On bipolar cells using single cell RT-PCR.
• Identified interacting proteins of metabotropic glutamate receptor 6 using conventional yeast two-hybrid screening and DUAL membrane yeast two-hybrid system.

Nationwide Children’s Hospital Columbus, OH
Ph.D. student 2009
• Confirmed interaction between mGluR6 and target interacting protein in cultured cells using Co-immunoprecipitation and immunocytochemistry.
• Quantified expression level of target genes in cultured cells using qPCR analysis.

University of Rochester Rochester, NY
Research Assistant 2006-2007
• Identified expression profile of regulatory proteins in rat ON bipolar cells using single cell RT-PCR and western immunoblotting.
• Characterized physiological function of regulatory proteins using SCG reconstitution system, whole cell patch-clamp technique and gene knockdown by siRNA.

West China University of Medical Sciences Chengdu, China
Pharmacy student 2000
• Therapeutic Drug Monitoring: controlled Cyclosporin A of patients received kidney transplantation.
• Detected Cyclosporin A or Benzbromarone in blood sample using HPLC.
• Withdrew blood samples from patients with kidney transplantation, processed samples to separate serum and plasma, and extracted Cyclosporin A or Benzbromarone from the blood samples.
• Analyzed HPLC data to evaluate efficacy and safety of domestic Benzbromarone.

TEACHING AND WORKING EXPERIENCE

Kent State University Kent, OH
Graduate Assistant 2003-2009
• Lab assistant in medical physiology for medical students.
• Lab assistant in biochemistry for undergraduate students.
• Lab assistant in microbiology and virology for medical and pharmacy students.
• Teaching assistant in medical pharmacology for graduate students.
• Teaching assistant in pharmacoinfomatics for graduate students.
• Teaching assistant in seminar of pharmacology for graduate students.
• Tutor in biochemistry for nursing students.

People’s Hospital of Sichuan Province Chengdu, China
Clinical Pharmacist 2000-2002
• Prepared medications according to physician prescriptions
• Detected therapeutic incompatibilities in prescriptions
• Dispensed medications by compounding, packaging and labeling of pharmaceutical products
• Provided pharmacological information to health care professionals
• Counseled patients on drug therapies

EDUCATION
Kent State University Kent, OH
Ph.D. in Pharmacology 2003-2009, awarded May 2010
Topic of Dissertation: G protein coupling and regulation of metabotropic glutamate receptor 6


West China University of Medical Sciences Chengdu, China
B.S. in Clinical Pharmacy 1995-2000 Topic of research: evaluation of the efficacy and safety of domestic Benzbromarone in the treatment of hyperuricaemia (using randomized controlled clinical study)

PUBLICATIONS

Ligr, M., Patwa, R.R., Daniels, G., Pan, L., Wu, X., Li, Y., Tian, L., Wang, Z., Xu, R., Wu, J., Chen, F., Liu, J., Wei, J., Lee, P., (2011) Expression and Function of Androgen Receptor Coactivator p44/Mep50 in Ovarian Cancer. PLoS ONE. 2011.
Tian, L., Ligr, M., Daniels, G., Zhou, L., Li, Y., Wu, X.,Wang, J., Melamed, J., Wang, Z., Lee, P., Structural and Functional Domains of AR cofactor p44 Mediating Growth Inhibition and AR Activation in Prostate Cancer. (Manuscript in preparation).
Daniels, G., Zhou, A., Tian, L., Gellert, L., Melamed, J., Li, Y., Zhang, D., Meruelo, D., Logan, S., Basch, R., Lee, P., Expression and function of TBLR1 in androgen-dependent and androgen-independent prostate cancer (manuscript in preparation).
Tian, L., Kammermeier, P.J.and Cismowski, M.J. Identification and characterization of CIP98 as a novel regulator of mGluR6 (manuscript in preparation).
Tian, L., and Kammermeier, P.J. (2006) G protein coupling profile of mGluR6 and expression of G alpha proteins in retinal ON bipolar cells. Visual Neuroscience. 2006 Nov-Dec; 23(6):909-16.
Tian, L. NK-2 Tachykinin receptor (2009). xPharm: The Comprehensive Pharmacology Reference; Elsevier Science, Inc., New York, 2009. doi:10.1016/B978-008055232-3.63783-3.
Tian, L. GR159897 (2009). xPharm: The Comprehensive Pharmacology Reference; Elsevier Science, Inc., New York, 2009. doi:10.1016/B978-008055232-3.61842-2.
Tian, L. GR94800 (2009). xPharm: The Comprehensive Pharmacology Reference; Elsevier Science, Inc., New York, 2009. doi:10.1016/B978-008055232-3.61847-1.

ABSTRACTS

Tian, L., Kammermeier, P.J.and Cismowski, M.J. (2008) Identification and characterization of CIP98 as a novel regulator of mGluR6 The FASEB Journal. 2008;22:726.6. (conference publication).
Tian, L., Cismowski, M.J., and Kammermeier, P.J. (2007) Identification of proteins interacting with mGluR6. The FASEB Journal. 2007 Apr; 21(6): A791-A791. (conference publication).

CONFERENCE PRESENTATIONS

Tian, L., Ligr, M., Daniels, G., Zhou, L., Li, Y., Wu, X., Wang, J., Melamed, J., Wang, Z., Lee, P., (2011) Structural and Functional Domains of AR cofactor p44 Mediating Growth Inhibition and AR Activation in Prostate Cancer. Department of Defense, Innovative Minds In Prostate Cancer Today (IMPaCT) Meeting. March 2011, Orlando, FL.
Tian, L., Kammermeier, P.J.and Cismowski, M.J. (2008) Identification and characterization of CIP98 as a novel regulator of mGluR6. Experimental Biology 2008. April 2008, San Diego, CA. Recipient of ASPET travel award.
Tian, L., Kammermeier, P.J. and Cismowski, M.J. (2008) Identification and characterization of CIP98 as a novel regulator of mGluR6. Kent Research Conference, Kent, OH. Awarded outstanding poster in competition.
Tian, L., Kammermeier, P.J.and Cismowski, M.J. (2007) Identification and characterization of interacting proteins of mGluR6. Experimental Biology 2007. April 2007, Washington DC.
Tian, L., Cismowski, M.J., and Kammermeier, P.J.(2006) Identification of proteins interacting with mGluR6. Biomedical Sciences Research Day, Rootstown, OH.
Tian, L., Cismowski, M.J., and Kammermeier, P.J.(2006) Identification and characterization of interacting proteins of mGluR6. Society for Neurosciences Annual Meeting. October 2006, Atlanta GA.
Tian, L., and Kammermeier, P.J. (2005) Coupling profile of mGluR6. Society for Neurosciences Annual Meeting. November 2005, Washington DC.

AWARDS

Postdoctoral Prostate Cancer Training Award (Department of Defense, 2010)
Frank G. Standaert Award (ASPET, 2008)
Outstanding Poster Award, Research Conference of the School of Biomedical Sciences (Kent State University, 2008)
Kent State University Research Grant (Kent State University, 2008)

ADDITIONAL SKILLS

Computer skills: Igor, GraphPad Prism, Adobe Acrobat photoshop, LSM Image Browser, Image J, Microsoft PowerPoint, Microsoft Excel, Microsoft Word, Instat
Language skills: Chinese Mandarin (native) and English (proficient)

PROFESSIONAL AFFILIATIONS

Member, SfN (Society for Neuroscience) 2005-2008
Member, ASPET (The American Society for Pharmacology and Experimental Therapeutics) 2005-2008
Member, AAAS (American Association for the Advancement of Science) 2007-2008

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