Yuwei Wang

Department of Pharmaceutics, University of Utah

Email: wpqfdd@r.postjobfree.com

20S 2030E, Bldg 570 (BPRB) Room 194

Phone: 801-***-**** (office)

Salt Lake City, UT 84112-5820 801-***-**** (cell)

EDUCATION

2006 – present PhD Candidate, Pharmaceutical Chemistry, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT

2003 – 2006 MS, Medicinal Chemistry, Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Peking University, Beijing, P. R. China

1999 – 2003 BS, Pharmaceutical Science, School of Pharmaceutical Sciences, Peking University, Beijing, P. R. China

RESEARCH EXPERIENCES

2006 - present Graduate Research Assistant, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT

Apply RNA interference technology to replace current treatments for osteoclast-mediated excessive bone resorption

o Express recombinant murine sRANKL in E.coli and osteoclast and osteoblast cultures

o Determine knock-down of cellular targets and screen small interfering (si)RNA with the highest efficacy

o Fabricate siRNA/bPEI-incorporated PLGA particles of certain sizes for siRNA-specific target delivery to phagocytic cells

o Modify calcium bone cement with siRNA-containing PLGA particles for local siRNA delivery

o Evaluate in vitro knockdown effects on osteoclast and osteoblast cell proliferation, differentiation, bone resorption and bone mineralization activities

Inhibit implant fibrous encapsulation by device-based local delivery of siRNA against mammalian target of rapamycin (mTOR) in a murine subcutaneous implant model

o Fabricate siRNA/bPEI encapsulated PEG-based hydrogel

o Design mouse subcutaneous siRNA-releasing device implantation

o Histological analysis of foreign body response and immunohistochemical analysis of target protein expression

Compartmental and non-compartmental PK analysis using WinNonlin

2003 - 2006 Graduate Research Assistant, Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Peking University, Beijing, P. R. China

Purify and characterize therapeutic anti-thrombosis and thrombolysis protein components in Agkistrodon halys pallas snake venom

o Purify proteins with highest bio-activities using chromatography, FPLC and HPLC

o Determine protein molecular weight and N-terminal sequence

o Test bioactivities of venom-derived fractions in Wistar rat models

o Synthesize RGD-containing peptides

SCIENTIFIC AND TECHNICAL SKILLS

Molecular Biopharmaceutics:

o Cell culture, cytotoxicity and cell proliferation assays, phagocytosis analysis

o DNA/RNA/protein analysis, PCR, real-time PCR, SDS-PAGE, western blotting, ELISA, cloning, protein expression, protein purification, chromatography, HPLC, FPLC

o siRNA transfection, cell fluorescent microscopic imaging

Particle Fabrication and Drug Release Assays:

o Nano-sized/micro-sized polymer PLGA particles, double-emulsion solvent evaporation technique

o Particle size distribution & zeta potential analysis, SEM

o Formulation and release assays for siRNA in simulated biological milieu

Animal Preclinical Studies: anti-platelet aggregation assay, anti-thrombosis assay, mouse bone marrow cell harvest, mouse subcutaneous implantation, histological and immunohistochemistry analysis

Computer Literate: Thoroughly skilled in using Microsoft applications, such as statistical analysis using Excel, ChemDraw, WinNonlin

Independent scientific experimental design and conduct, technical reporting, critical evaluation, manuscript preparation, publishing

OTHER EXPERIENCES

2010-2011 Invited Reviewer Expert Opinion on Therapeutic Targets Journal (EOTT)

International Journal of Genetics and Molecular Biology

2009 - 2010 Teaching Assistant, Taught Drug Delivery and Dosage Forms (PHCEU 5125), Spring semesters, School of Pharmacy, University of Utah, UT

2008 - 2010 Supervisor, undergraduate research project, Dr. Grainger’s lab, University of Utah, Salt Lake City, UT

2009 Student Project Judge, Salt Lake Valley Science & Engineering Fair, Salt Lake City, UT

2008 - 2009 Graduate Admissions Committee, Program of Molecular Biology and Biological Chemistry, University of Utah, Salt Lake City, UT

2003 - 2006 Laboratory Manager, Protein purification lab, Department of Medicinal Chemistry, Peking University, Beijing, P. R. China

2004 Teaching Assistant, Taught Medicinal Spectroscopy course, Health Science Center, Peking University, Beijing, P. R. China

2003 Summer Internship, Department of Pharmacy, People’s Hospital, Beijing, P. R. China

PRESENTATIONS

1. Yuwei Wang, David W. Grainger. Using small interfering RNA to knock down the molecular target of alendronate in both osteoclasts and osteoblasts. The 6th Annual Mountain West Biomedical Engineering Conference. Park City, UT, 2010 Poster presentation.

2. Yuwei Wang, David W. Grainger. Inhibiting osteoclast resorption of bone using small interfering RNAs targeting RANK. AAPS Annual Meeting. Los Angeles, CA, 2009 Poster presentation.

3. Yuwei Wang, Alexandra Buffington, David W. Grainger. Inhibiting osteoclast resorption of bone using small interfering RNAs targeting RANK. Canyons Biomedical Conference. Park City, UT 2008 Poster presentation.

4. Yuwei Wang, David W. Grainger. Inhibiting osteoclast resorption of bone using small interfering RNAs. The 3rd Annual Mountain West Biomedical Engineering Conference. Park City, UT 2007 Poster presentation.

HONORS AND SCHOLARSHIPS

2009, 2010 UU Graduate School Travel Assistance Grant, University of Utah, Salt Lake City, UT

2007-present Research Assistantship, Department of Pharmaceutics, University of Utah, Salt Lake City, UT

2006 Fellowship, Molecular Biology and Biological Chemistry Graduate Program, University of Utah, Salt Lake City, UT

2001-2002 Tian Yao Fellowship, College of Pharmaceutical Science, Health Science Center, Peking University, Beijing, P. R. China

PATENTS AND INVENTIONS

1. David W. Grainger, Yuwei Wang. Method for enhancing bone mineralization by inhibiting osteoclast formation and bone resorption using small interfering RNAs targeting RANK and other bone-resorbing signaling pathways. University of Utah Technology Transfer Office, 2009

2. Shiqi Peng, Ming Zhao, Chao Wang, Yuwei Wang. Purification and preparation and application of a antithrombotic double-chain peptide from Agkistrodon halys pallas venom. Chinese Patent Application number: CN200610144241.9, 2006

3. Shiqi Peng, Ming Zhao, Chao Wang, Yuwei Wang. Purification and application of an acidic phospholipase A2, which having anticoagulant activity from Agkistrodon halys pallas venom. Chinese Patent Application number: CN200610087259.X, 2006

PUBLICATIONS

1. Yuwei Wang, David W.Grainger. RANK siRNA encapsulated in PLGA particles released from injectable calcium phosphate cement to target osteoclasts effectively improve osteoporotic bone regeneration by inhibiting osteoclast-mediated bone resorption. (In preparation)

2. Yuwei Wang, David W.Grainger. Cellular targets of small interfering RNA to attenuate osteoclast-mediated excessive bone resorption. Review. (In preparation)

3. Yuwei Wang, Alexandra Panasiuk, David W. Grainger. Small Interfering RNA Knocks Down the Molecular Target of Alendronate, Farnesyl Pyrophosphate Synthase, in Osteoclast and Osteoblast Cultures. Molecular Pharmaceutics (2011) (Accepted, In press)

4. Hironobu Takahashi,* Yuwei Wang,* David W. Grainger. Device-based local delivery of siRNA against mammalian target of rapamycin (mTOR) in a murine subcutaneous implant model to inhibit fibrous encapsulation. J. Controlled Release (2010); 147:400-407. *co-first authors

5. Yuwei Wang, David W. Grainger. siRNA knock-down of RANK signaling to control osteoclast-mediated bone resorption. Pharm Res (2010); 27(7):1273-84.

6. Yuwei Wang, Guohui Cui, Ming Zhao, Jian Yang, Chao Wang, Roger W.Giese, Shiqi Peng. Bioassay-directed purification of an acidic phospholipase A2 from Agkistrodon halys pallas venom. Toxicon (2008); 51:1131-1139.

7. Yuwei Wang, Ming Zhao, and Shiqi Peng. The Pharmacological Foundation of ACEI and ARB. Great Wall Cardiology Express (2005); 3(2):34-36.

PROFESSIONAL AFFILIATIONS

Member, American Association of Pharmaceutical Scientists (AAPS)

REFERENCES

Available upon request

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