Curriculum Vitae

Priyanka Kushwaha we64xh@r.postjobfree.com

G-401, Jagriti apartment Mobile +91-966*******

Bangalore, India

JOB OBJECTIVE

To practice quality in every assignment and achieve organizational goals along with personal goals by working in a competitive and challenging environment.

PROFILE

Total Work Experience: 4.3 Years

Skill Set: Isolation and pure culture formation of bacteria, Characterization of unknown bacterial population, General and Applied Microbiology for microbial risk assessment of “Water Treatment Devices”, Standards and Guidelines for foods and drinking water ( HACCP, ISO 22000, USEPA Guidelines) Microbiological aspects of Foods and HPC products; Micro Risk Assessment for Ice-cream products at Ultra low temperature; Virus/ Vibrio cholera (Rugose morphology) inactivation kinetics; standardization of molecular methods for waterborne pathogens and risk assessment of Algae in source water.

ORGANISATIONAL EXPERIENCE

Hindustan Unilever Limited From July’2008 to till now

Designation: Senior Research Executive

Role:

• Isolation of microbes and pure culture formation from a mixed population.

• Fully hands-on testing microbiological safety of raw materials used in HHC (House Hold Care) and water/foods.

• Develop scientific approach for robust water treatment technologies in the team

• Risk assessment and benchmarking of competitor products in the areas of “Antibacterial Soap/hard surface cleaners” Water treatment technologies” and food processing method”

• Assessment of antimicrobial efficacy of Surface cleaner/soap formulation including bacteria/virus.

• Develop molecular methods for rapid identification of water / food borne pathogens.

KEY PROJECTS and DELIVERIES

• Project: Development and optimization of Antiseptic Liquid formulation

KEY DELIVERABLES:

Identification of effective components of formulation.

Isolation of bacteria from volunteer hands (both the hands) and base line count for the assay development.

Standardizatition of antimicrobial assay for target bacteria/virus for the formulation

• Project: Development of Hard surface cleaner (Cif project)

KEY DELIVERABLES:

Define performance window of “New Cif Formulation” for gram Positive/negative bacteria

Consistency in product performance in Clean and dirty condition under different physical parameters (pH, Temperature).

Defining the criticality of the steps involved in the technology.

• Project: Non-Disruptive method for biofouling assessment

KEY DELIVERABLES:

Isolate and pure culture formation of bacteria from biofouled membrane.

Characterization of all the organisms from pure culture.

Assessment of biofouling efficiency of individual bacteria as well as bacterial cocktail.

Development and standardization of method to assess biofouling of membrane on the basis of pressure increase on the membrane.

• Project: High throughput method for MS2 Bacteriophage quantification

KEY DELIVERABLES:

Define and Standardize MS2 host culture age (E.coli) for effective infection by Phage.

Standardization of culture dilution to get infected cell count for unknown MS2 bacteriophage concentration

Full proof SOP for MS2 bacteriophage quantification and its practical application.

• Project: Very low cost “Water Treatment Technology”:

KEY DELIVERABLES:

Define performance window of “Sachet Formulation” in line with USEPA Guidelines.

Consistency in product performance under different water quality parameters.

Defining the criticality of the steps involved in the technology.

• Project: Development and optimization of filtration technique for Cryptosporidium cyst from drinking water.

KEY DELIVERABLES:

Characterization of cyst structure and Zeta potential.

Development and optimization of PLEATED FILTERS for cyst filtration from source water as per USEPA and NSFP231 guidelines.

• Project: Kill kinetics and Mathematical modeling of Resistant waterborne pathogens (ENTERIC VIRUS and Vibrio cholera)

KEY DELIVERABLES:

Standardized a high throughput method to quantify MS2 Bacteriophage.

Physiological conversion of Vibrio (N16961) Smooth strain in to Rugose strain.

Data generation and modeling of kill kinetics pattern of MS2 Bacteriophage and Vc Rugose strain with chlorine under different physical parameter.

Determination of Aggregate size of Vc Rugose by using PSD and DLS to understand the resistance for certain specific population.

Research paper for publication (WR 1925) is under process in Water Research.

• Project: Emerging waterborne pathogens and development of molecular techniques.

KEY DELIVERABLES:

Identification of key bacteria of Enterobacteriacae using molecular biological techniques

Primer validation and standardization.

Demonstrate “Proof of concept” in bacteria (different strains of bacteria) cocktail.

Highlights

• Hold a patent (Assigne-Hindustan Unilever Research Centre) for water purification device 10175259.0-1213.

• Manuscript (Water Research1925) for a Research Paper on “A modified Ct-criterion for virus inactivation in the presence of decaying chlorine concentration” has been submitted.

• Presented a paper in an International conference on Sustainable water technologies in NEERI, Nagpur on “A modified Ct-criterion for virus inactivation in the presence of decaying chlorine concentrations” in 2011.

• Awarded by Hindustan Unilever research centre for outstanding contribution to continuous improvement for Filter design enabling higher efficacy and superior consumer benefit.

• Delivered seminar on “Difference of inactivation kinetics for two physiological different strain of Vibrio cholerae” in Unilever UK , Colworth laboratory.

Mathura Veterinary University,Mathura From Nov’2007 to June 2008

Designation : Senior Research Fellow (SRF)

I have worked on Department of Biotechnology (DBT) funded project on “Niche Area” to Identify Disease outbreaks in animals Especially Foot and Mouth Disease (FMD).

Role:

• Literature scanning and identifying key data gaps in molecular methods for FMD virus.

• Standardization of Nested PCR and RT- PCR for FMD virus.

• RNA isolation and SDS PAGE.

• Design primers and Development of a robust Molecular method to detect FMD.

• Standardization of primers and methods.

ACADEMIC CREDENTIALS

• Post-Graduate in Microbiology with 75% from Bundelkhand University, Jhansi, India in 2007.

• Bachelor of Science in Industrial Microbiology with 67% from Deen Dayal Upadhyay University, Gorakhpur India in 2005.

• Successfully completed Eight-month summer training program of Post-Graduation on “Detection of Salmonella Enterica Serovar typhi in chronic typhoid carriers state in normal population and its molecular characterization” in Banaras Hindu University, Varanasi, India

• M.Sc. Topper of (Microbiology branch) Bundelkhand University, Jhansi (2007).

• Attended a workshop on “Biofilm and identification methods on the membranes”, Biofilm Engineering Institute Montana, USA (March 2010).

• Attended a workshop on “Sustainability and product development” (June 2011).

• Attended a workshop on “COES assessment for projects”, Unilever UK , Colworth lab, (September 2011).

REFERENCE

1. Dr. Morris Waskar

Research Scientist

Hindustan Unilever Research Centre, Bangalore, India

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2. Dr. Moira Johnston

Senior Research Scientist

Unilever R & D, Colworth, UK.

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