Hrushikesh B. Agashe, Ph. D

*****, *. ************ ******, *** # 400, Oklahoma city, Oklahoma 73120

Mobile # 313-***-****, Email: v6z1l0@r.postjobfree.com

Summary of qualifications:

Offering comprehensive experience and expertise in the following areas:

• 3+ years of hands on experience in development and characterization of protein formulations

• Experience with in vitro and in vivo evaluation of protein formulations in cells and animals

• Successfully developed protein formulations like liposome encapsulated hemoglobin

• Expertise in formulation development of novel systems like liposomes and nanoparticles containing hydrophobic anticancer drugs

• Expert in formulation development of polymeric drug delivery systems like polymer-drug complex/conjugates, their physiochemical characterization, in vitro and in vivo evaluation

• Experience with solubility enhancement studies using cyclodextrin and dendrimer

• Hands on experience of working with small animals for organ distribution and pharmacokinetics

• Ability to independently plan research projects, interpret data and to draw meaningful conclusions

• Ability to work in multidisciplinary team

Professional experience: Post-doctoral scholar

Oklahoma University Health science center, OK City, OK, USA (May 2007-Present)

Summary of projects handled

• Development, characterization and evaluation of protein formulations (NIH funded project): Development of Liposome Encapsulated Hemoglobin (LEH) formulation, physicochemical

and functional characterization of LEH, in vitro toxicity studies in HUVEC cell lines, in vivo efficiency evaluation in 40% hemorrhagic shock model in rats

• Development, characterization and evaluation of liposomal anticancer agents: Development of liposome containing hydrophobic anticancer agents, physicochemical characterization, in vitro cytotoxicity studies in H441 lung and PC3 prostate cancer cell lines, in vivo antitumor activity evaluation in xenograft lung cancer model in nude rats

• Development of nanoparticles for anticancer drugs (ongoing project): This project is at optimization stage. Our ultimate goal is to develop peptide/antibody targeted nanoparticles for cancer targeting.

• Solubility Enhancement: Solubility enhancement studies on hydrophobic drugs using cyclodextrins and dendrimers, phase solubility analysis, formation and characterization of inclusion complexes by DSC and XRD, encapsulation of inclusion complexes into liposome for drug delivery

Wayne State University, Detroit, MI, USA (May 2006-April 2007)

• Responsibility: Formulation development of polymer based drug delivery systems Summary: Formation and characterization of dendrimer-drug conjugates dendrimer-drug complexes, PEGylation of dendrimers.

Education

Ph. D: Pharmaceutical Sciences, 2006, Department of Pharmacy, Dr. H. S. Gour University, Sagar, M.P., India

Dissertation topic: Poly (propylene imine) dendrimer based molecular containers for delivery of antimalarial bioactives

M. Pharm: Pharmaceutics, 2000, University of Pune, Maharashtra, India

B. Pharm: 1998, Dr. B. A. M. University, Maharashtra, India

M. B. A., 2002, Dr. B. A. M. University, Maharashtra, India

Instrumentation skills

EmulsiflexC3, Avestin, Canada (high-pressure homogenizer), Lipex Extruder, Triad Freeze dryer, Labconco USA, Optima L100 XP Ultracentrifuge, Beckman coulter, USA, Cross flow filtration assembly FiberFlo, Minntech, USA, Particle sizer and ZetaPlus, Brookhevan Instruments, USA

Awards

Junior research fellowship (University grants commission, N. Delhi, India) 2003-2006

Computer proficiency

MS Office, ISISI draw, Endnote

Recent publications

H. B. Agashe, P. Lagisetty, S. Awasthi and V. Awasthi, (2010) ‘Improved formulation of liposome encapsulated hemoglobin with an anionic non-phospholipid’ Colloids and Surfaces B: Biointerfaces 75 (2) 573-583.

H. B. Agashe, P. Lagisetty, B. Grady, V. Awasthi, (Communicated) ‘Liposomal curcumin analog 3,5-bis(2-fluorobenzylidene)-4-piperidone: Preparation and in vitro characterization. J. Liposome Research.

V. Awasthi, H. B. Agashe, S. Doblas, R. Towner, (in press) ‘Magnetic Resonance Spectroscopy for Evaluation of Liposome-Encapsulated Hemoglobin as Resuscitation fluid’ Artificial Cells, Blood Substitutes, and Biotechnology An International Journal.

G. Pathuri, H. B. Agashe, V. Awasthi, H. Gali (in press) Radiosynthesis and in vivo evaluation of F-18-labeled pancreatic islet amyloid inhibitor hIAPP. Journal of Labeled Compounds and Radiopharmaceuticals.

H. B. Agashe, A. K. Babbar, S. Jain, R. K. Sharma, A. K. Mishra, A. Asthana, M. Garg, T. Dutta, N. K. Jain (2007) Investigations on biodistribution of technetium-99m-labeled carbohydrate-coated poly(propylene imine) dendrimers. Nanomedicine: Nanotechnology, Biology, and Medicine 3: 120– 127.

H. B. Agashe, T. Dutta, M. Garg, N. K. Jain (2006) Investigations on the toxicological profile of functionalized fifth-generation poly (propylese imine) dendrimer. The Journal of pharmacy and pharmacology. The Journal of pharmacy and pharmacology, 58(11): 1491-8.

U. Gupta, H. B. Agashe, N. K. Jain (2007) Poly (propylene imine) dendrimer mediated solubility enhancement: effect of pH and functional groups of hydrophobes. Journal of pharmacy & pharmaceutical sciences, 10(3): 358-67.

P. Vijayrajkuar, H. B. Agashe, T. Dutta, N. K. Jain (2007) PEGylted dendritic architecture for development of a proloned drug delivery system for an antitubarcular drug. Current Drug Delivery 4(1): 11-19.

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