JIAN MA

*** ******* **. ****** ****, NC *****

540-***-**** (C) / uyu04b@r.postjobfree.com

OBJECTIVE

I am seeking molecular biological position. I hold a Master’s degree in Biochemistry and Molecular Biology and have years of academic and industrial experience which trained me to work resourcefully and independently as well as contribute to team projects. I have a positive attitude and willingness to adapt to change coupled with hard work and readiness to assume responsibility and accountability.

QUALIFICATIONS SUMMARY

Biotechnology & Pharmaceutical R&D: Hands on experience with:

 DNA: DNA Isolation, Purification, DNA cloning (Recombinant DNA ) and sequencing, Restriction

Enzyme Digestion, Picogreen, PCR, RT-PCR, Gel Electrophoresis, Southern Blot, DNA transformation

 RNA: RNA Extraction and Purification (column and HPLC), qRT-PCR, In-vitro transcription, Ribogreen

 Protein: BCA, SDS-PAGE, Western blotting

 Others: Histology, Immunostaining, FISH, Tissue culture, Radioactive isotope (P32), Microscopy

 Models: Alphavirus, human plasma, Mice (surgery and tissue sectioning), E.coli, Yeast, Drosophila

Computer Skills:

 Microsoft Office, Adobe Photoshop, BLAST, DNA sequence analysis software (Sequencher and Vector NTI)

PROFESSIONAL EXPERIENCE

AlphaVax, Inc. --- Development Associate Scientist II Aug. 2009 – Present

Project: 1. Investigate the efficiency of incorporation of the m7G Cap Analog for in vitro transcription reaction

2. Optimize the HPLC purification of RNA

 HPLC purification of RNA and oligonucleotides

 Digest the uncapped mRNA with 5’-phosphate-dependent exonuclease and optimize the reaction condition

 Perform oligo and RNA hybridization and digest with different ribonucleases and endonucleases (S1 nuclease, P1 nuclease, RNase H et.,)

 Quantify the mRNA by using quanltative real-time fluorogenic PCR

Talecris Biotherapeutics ---Associate Research Scientist Jan. 2008 – Aug. 2009

Project: 1. Development of NAT assays for detection of human virus in human plasma

2. Development of highly efficient purification of human viral RNA and DNA from human plasma

 Responsible for hands-on development of a qualitative real-time fluorogenic PCR assays for detection of HCV and HIV in human plasma donations

• Prepare HCV genome sequence alignment to identify most conserved sequence regions within the

5’ UTR for primer and probe design

• Evaluate multiple primer/probe sets

• Optimize PCR master mix formulation and thermal cycling conditions

• Construct HCV amplification and internal controls by in-vitro transcription

 Develop one-tube viral RNA and DNA extraction and purification method from human plasma

 Contributor to the reagent formulation project: formulation of PCR reagents for the HBV, HCV, and HIV assays

• Responsible for evaluation and optimization of Taq DNA polymerase and reverse transcriptase

from different manufacturers as alternate enzymes for the HCV detection assay

 Experienced in data analysis and interpretation, writing reports and presenting data at meetings

 Experienced with 7300 Real-Time PCR System, Bio-Rad IQ Real Time PCR System, GeneAmp PCR system 9700, Nanodrop Spectrophotometer and Sub-Cell GT Agarose Gel Electrophoresis System

 Familiar with functions of a laboratory performing under cGMP regulations

University of Tennessee---Research Assistant Mar. 2004-Aug. 2007

Project: Investigate a gene’s function in chromosome segregation and two proteins interaction

 Identify a new gene’s function on proper Chromosome segregation in Meiosis

• Generation of clones and transgenic Drosophila

• Designed probe and performed FISH & Immunostaining to analyze meiosis process using Confocal

Microscopy

 Determine the protein-protein interaction by setting up a yeast-two hybrid system

• Designed primers and constructed expression vectors (plasmid)

• Carried out E.coli and yeast transformation and extracted and purified plasmid DNA

• Proved the protein-protein interaction by performing reporter gene expression assay

• Confirmed the activity of proteins using SDS-PAGE and Western Blotting

 Utilized Southern blot and cDNA screen on Drosophila genome cDNA library

ShangHai Reproductive Medicine Key Laboratory---Research Assistant Sept. 2001-Feb. 2004

Project: Explore the bacterial infection in male reproductive system with human infertility

 Performed bacterial infection and transfection on mice for infertility research

 Measured the sperm activity

 Assisted in growing and harvesting cell culture and maintained cell line

 Hands on experience with surgery preparation/anesthesia and tissue sectioning on mice and rat

EDUCATIONAL BACKGROUND

MS Degree in Biochemistry, Cellular & Molecular Biology Aug. 2007

UNIVERSITY OF TENNESSEE, Tennessee GPA 3.6

Bachelor of Medicine Aug. 1999

NANJING MEDICAL UNIVERSITY, China GPA 3.5

~ Medical Doctor at Nanjing Brain Hospital (1999-2001)

~ References Available upon Request ~

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