Raj Pandit

u5y2pf@r.postjobfree.com

310-***-****

EDUCATION

California State University, East Bay M.S. Biochemistry

University of California, Los Angeles B.S. Biochemistry

EXPERIENCE

Life Technologies

Scientist I, Production

September 2011 - Present

Functional testing of Invitrogen molecular biology products.

Bacteria cell culture, transformation, restriction enzyme digestion, cloning, site directed mutagenesis.

Prokaryote, eukaryote and plant genomic DNA and RNA isolation, plasmid purification.

cDNA library construction, plasmid preparation, PCR, qPCR, RT-PCR, nucleic acid quantitaion

SDS-PAGE, western blotting, HPLC, gel electrophoresis

Troubleshot assays, report results and present data in weekly meetings.

California State University, East Bay

Graduate Student, Biochemistry

February 2009 - December 2010

Hands on experience in cell culture, protein and analytical chemistry.

Primary culture of mammalian cells, cell line maintenance, cloning, transfection, contamination testing.

Protein purification/characterization, affinity chromatography, activity assays, western blotting, HPLC.

UV/VIS, IR Spectroscopy, GC/MS.

LabCorp

Quality Control Associate

September 2005- February 2009

Manufacture and testing of reagents and controls used in PCR based viral assays.

Viral DNA/RNA extraction, PCR/RT-PCR, TaqMan qPCR.

Southern Blotting, immunostaining, SDS-PAGE, gel electrophoresis.

Initiate, investigate, and close deviation records per quality system procedure.

Streamline work flow and procedures to increase efficiency and improve quality.

Implement corrective and preventative actions in response to observed trends.

Develop and validate Excel spreadsheets and macros used in information systems.

Schedule and perform PM, calibration and troubleshooting of laboratory equipment.

Execute IOPQ protocols for validation of new equipment.

LabCorp

Laboratory Technician

June 2003 - September 2005

Perform FDA licensed, PCR viral assays.

Viral DNA/RNA extraction, PCR/RT-PCR, Southern Blotting, Hybridization and qPCR.

Track samples, manage specimen database.

Maintain laboratory, prepare buffers and dilutions, document all procedures.

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