Yingyun Cai, Ph.D.

*** ************* ** *** ***, Rockville MD 20852

202-***-****, ogmwfe@r.postjobfree.com

PROFESSIONAL PROFILE

•12+ years of hands-on laboratory research experience in Virology, Molecular Biology, Immunology, Antibody Development, Protein Expression and Purification, Cell Culture

•Highly motivated and dedicated with ability to analyze data and develop experimental plan to solve scientific problems

•Experience with Biostatistics analysis and Epidemiology study

•Can work independently or as an excellent team player with effective oral and written skills

EDUCATION

•University of Arkansas for Medical Sciences, Little Rock, AR

Ph.D., Virology, 2000-2006

•Shanghai Jiao Tong University, Shanghai, P. R. China

M. S., Biochemistry and Molecular Biology, 1998-2000

B. S., Biotechnology, 1994-1998

B. S. (minor), Computer Science, 1994-1998

RESEARCH EXPERIENCE

Postdoctoral Fellowship 2006-Present

Mentor: Dr. Ed. Berger, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Disease (NIAID), National Institutes of Health (NIH), Bethesda MD

Project: Development of Immunotoxins as Novel Therapeutic Strategies for Kaposi's Sarcoma Associated Herpesvirus (KSHV) related Multicentric Castleman’s Diseases (MCD)

•Constructed, expressed and affinity purified KSHV glycoproteins

•Generated and characterized monoclonal antibodies for binding affinity and viral neutralization

•Developed immunotoxins specific for KSHV glycoproteins for KSHV related MCD

•Designed chimeric antigen receptors specific for KSHV glycoproteins as an alternative strategy

•Established real-time PCR assay to detect intact KSHV viral particles in vitro

•Characterized KSHV glycoproteins mediated fusion assay

•Screened EBV-LMP1 specific hybridoma cells by high-through put flow cytometry

•Supervised and mentored NIH postbac IRTA fellows with regard to the design, execution and analysis of projects

•Collaborated with intramural and extramural research groups

Ph. D. Research 2000-2006

Advisor: Dr. Xuming Zhang, Department of Microbiology and Immunology, University of Arkansas for Medical Science, Little Rock, AR

Thesis: Characterization of Cellular Gene Expression by Coronavirus Infection and the Role of

Cellular Proteins in Coronavirus Replication.

•Investigated the cellular gene expression profile upon murine coronavirus infection by microarray

•Characterized the role of MEK signal pathway on murine coronavirus replication

•Collaborated with colleagues to develop an infectious cDNA clone of a human enteric coronavirus

•Trained graduate students in laboratory techniques

•Managed inventory of basic laboratory supply and reagents

•Handled monthly radioactive lab testing and disposal of radioactive wastes

Master’s Degree Research 1999-2000

Advisor: Dr. Rongxiu Li, Shanghai Research Center of Biotechnology, Institutes for Biological Sciences, Chinese Academy of Sciences (CAS), Shanghai, P.R. China

Thesis: Cloning and Characterization of a Novel Zinc Finger Gene Expressed Restrictedly in Rat Brain

•Characterized the expression profile of rZic gene in the different anatomy sizes of rat

•Screened rZic interaction proteins by yeast two hybridization system

TECHNICAL SKILLS

•Virology: virus isolation, propagation, characterization, purification and titration, infectious cDNA cloning, reverse genetic system, pseudo viral particle production, viral fusion assay, viral neutralization assay

•Molecular Biology: DNA/RNA extraction, gene cloning, PCR (primer and probe design), RT-PCR, DNA sequencing and sequence analysis, microarray, RACE cDNA amplification, Real-Time quantitative PCR, site-directed mutagenesis, various reporter assays (luciferase, β-gal, CAT), defective interfering RNA system, siRNA gene knock down, transfection, transformation, cDNA library screening, SDS-PAGE, protein labeling, protein tagging, cell culture (mammalian, insect, yeast, E. coli)

•Immunology: Western Blot, immunoprecipitation, ELISA, immunofluorescence assay, FACSCan/FACSCalibur Flow Cytometry, cytotoxicity assay, cell-proliferation assay

•Antibody: antigen design, hybridoma single-cell cloning, monoclonal antibody screening, characterization, purification, epitope mapping, VH and VL segments cloning, Mab High-Throughput Screening (HTS), recombinant immunotoxin construction, expression, purification and charaterization

•Protein Expression: 293 Free style mammalian expression system, E. coli pET expression system, viral expression systems (vaccinia virus, baculovirus, adenovirus, lentivirus), yeast two hybridization system

•Protein Purification: ion exchange chromatography, size-exclusion chromatography, affinity chromatography, hands-on experience in AKTA Prime and FPLC

•Biostatistics Analysis: Z-test, F-test, t-test, Chi-Square test, Regression (linear, multiple), ANOVA (one-way, two-way), ANCOVA, survival analysis, sample size and power analysis

•Proficient with Microsoft Word, Excel, PowerPoint, software associated with FACs (Flow Jo, Cellquest) and AKTA explore (Unicorn)

•Familiar with Bioinformatics Software for DNA, RNA and protein sequence analysis

PUBLICATIONS

Cai, Y., Liu, Y., and Zhang, X. (2007) Suppression of coronavirus replication by inhibition of the MEK signaling pathway. J. Virol. 81:446-456.

Zhu, H., Liu, Y., Cai, Y., Yu, D., Pu, Y., Harmon, L. and Zhang, X. (2006) Toward the development of an infectious cDNA clone of a human enteric coronavirus. Adv Exp Med Biol. 581:527-530.

Cai, Y., Liu, Y., and Zhang, X. (2006) Induction of transcription factor Egr-1 gene expressing in cultured astrocytes by murine coronavirus infection. Virology. 355:152-163.

Cai, Y., Liu, Y., Yu, D., and Zhang, X. (2003) Down-regulation of transcription of the pro-apoptotic gene BNip3 in cultured astrocytes by murine coronavirus infection. Virology. 316:104-115.

Zhang, X., and Cai, Y. (2003) An overview on RNA-dependent RNA polymerases of RNA viruses. RNA Viruses: Mechanisms of Replication and Transcription, Transworld Press.

Liu, Y., Cai, Y., and Zhang, X. (2003) Murine coronavirus induces apoptosis in cultured rat oligodendrocytes during virus entry. J. Virol. 77:119**-*****.

Cai, Y., Kang, J., Liu, H., Liu, J. and Li, R. (2001) Cloning, sequencing and preliminary expression of novel Zinc finger gene expressed restrictedly in rat brain. Journal of Shanghai Jiao Tong University, 35:1071-1075.

MANUSCRIPTS

Cai, Y. and Berger, E.A. (2011) Generation and characterization of monoclonal antibodies directly against KSHV glycoprotein gH. (in preparation)

Cai, Y. and Berger, E.A. (2011) An immunotoxin targeting the gH glycoprotein of KSHV for selective killing of cells in the lytic phase of infection. Antiviral Research (revised and resubmitted)

Patent

Cai, Y. and Berger, E.A. (2011) Immunotoxins against glycoprotein H (gH) of Kaposi’s sarcomar- associated herpesvirus (patent application E-260-2010/0-US-01-423*-*****-**)

MEETING ABSTRACTS

Cai, Y., and Berger, E.A. (2010) Development of an immunotoxin directly against KSHV gH protein as a novel therapeutic strategy for KSHV-related multicentric Castleman’s disease. The 35th Annual International Herpesvirus Workshop, University of Utah, Salt Lake City, Utah.

Cai, Y., and Berger, E.A. (2009) Generation and characterization of monoclonal antibodies directed against KSHV glycoprotein gH. The 34th Annual International Herpesvirus Workshop, Cornell University, Ithaca, New York.

Liu, Y., Cai, Y., and Zhang, X. (2007) Role of transcription factor Egr-1 in murine coronavirus

replication. The 26th annual meeting of American Society for Virology, Oregon State University,

Oregon.

Cai, Y., and Zhang, X. (2006) The murine coronavirus replication is inhibited by ERK signal pathway inhibitor UO126. The 25th annual meeting of American Society for Virology, University of Wisconsin-Madison, Wisconsin.

Zhu, H., Liu, Y., Cai, Y., Yu, D., Pu, Y., Harmon, L. and Zhang, X. (2006) Toward the development of an infectious cDNA clone of a human enteric coronavirus. The 10th International Nidovirus Symposium, Colorado Springs, Colorado.

Cai, Y., and Zhang, X. (2005) Induction of transcription factor Egr-1 by murine coronavirus infection. The 24th annual meeting of American Society for Virology, Penn State University, PA.

Cai, Y., and Zhang, X. (2004) Up-regulation of transcription factor Egr-1 gene expression in DBT cells by murine coronavirus infection. Annual Meeting of South Central Branch of America Society for Microbiology, Mississippi State University, Mississippi.

Cai, Y., and Zhang, X. (2003) Down-regulation of the pro-apoptotic BNip3 gene in DBT cells by murine coronavirus infection. The IXth International Symposium on the molecular biology, evolution, immunology, and pathogenesis of Nidoviruses (Arteriviruses and Coronaviruses). Egmond aan Zee, the Netherlands.

Liu, R., Cai, Y., and Zhang, X. (2001) The 5’-penultimate nucleotide of the genome RNA of coronavirus mouse hepatitis virus revisited. Annual Meeting of South Central Branch of American Society for Microbiology. Baton Rouge, LA.

AWARDS

•Fellows Award for Research Excellence (FARE) from NIH, 2010

•Travel award to attend the 24th annual meeting of American Society for Virology, 2005

•Travel award to attend the IXth International Symposium on the molecular biology, evolution, immunology, and pathogenesis of Nidoviruses, 2003

•Grant award entitled “Regulation of BNip3 Gene Expression by Mouse Hepatitis Virus Infection” from University of Arkansas for Medical Sciences Graduate Student Research Fund (GSRF), 2002

PROFESSIONAL MEMBERSHIPS

•American Society for Virology

•American Society for Microbiology

ADDITIONAL TRAINING

•Sep, 2009-Dec, 2009 NIH FAES Introductory Toxicology (TOXI303)

•Sep, 2009-May, 2010 NIH FAES Introduction to Experimental Statistics (STAT 200)

•Sep, 2010-Dec, 2010 NIH FAES Introduction to Epidemiology (STAT 314)

•Sep, 2010-Dec, 2010 NIH FAES Introduction to Environmental Health Sciences (PBHL501)

•Sep, 2010-Present NIH FAES Principles of Clinical Pharmacology (PHAR500)

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