Curriculum Vitae

Personal Informations:

Name: Sumant Chaubey Contact:

Date of birth: 18 February,1980 Tel : +91-999*******,-890-***-****

Marital Status : Married igjjrj@r.postjobfree.com

Address:

B- 12 Akhanddeep Tenaments,

Atmjyoti Ashram Road, Ellora Park, Vadodara-390007

Key Skills

Protein purification type’s viz. size exclusion chromatography, affinity chromatography, ion exchange chromatography and hydrophobic interaction chromatography- Experience >2 years (industrial)

Upstream Purification steps like inclusion body dissolution, refolding of proteins, membrane based micro-filteration. Experience >2 years (industrial)

HPLC Experience on method development and validation-More than 5 year ( 4.5 year Ph.D + 1.5 Year Industrial)

HPLC – Isocratic and gradient, Optimization of mobile phase, selection of column and different kind of detectors.

Expert in analytical instrument and process validation and method development following USFDA.

Instruments validation list : UV-Visible spectrophotometer, RP-HPLC (WATER and SHIMADZU), HPLC column performance check and their calibration, Chromatography column and instrument performance check and method development.

Antibiotics(polyketides) bioassay method development: MRSA ,E.Coli, Salmonella Spp. >4 years experience(during Ph.D)

Analytical Methods and their result analysis viz. NMR, LC-MS, FT-IR, GC, GC-MS, HP-TLC etc.

Cell culture, Cell banking, Analytical assay validation, ELISA, Immunoelectrophoresis and gel diffusion, SDS PAGE electrophoresis analysis, Preparation of standards, Calibration of standards, Stability studies, Calibration of equipments and LAL test

Bacterial Identification by biochemical and molecular methods.

TOC Analyzer (shimadzu), cleaning validation according to US-FDA.

Expert in developing methods on - >1.5 years(industrial)

I. AKTA pilot: which ensure the good laboratory practice GLP and current good manufacturing practice cGMP demands for Phase I–III in drug development and final-scale production

II. AKTA Purifier: Purification of protein microgram, milligram or gram scale.

Electrophoresis- Agarose and Acrylamide based, Western and Southern blotting.

Bacterial gene cloning by transformation, electroporation and conjugation, restriction enzymes and its use in molecular biology. Experience ->5 year(1.5 years M.Sc. dissertation + 4.5 years Ph.D)

Basics of Polymerase Chain Reaction (PCR) and its modifications based on use. ->5 year experience (1.5 years M.Sc. dissertation + 4.5 years Ph.D)

Cell free enzymatic assay for the enzyme-glucose dehydrogenase(gdh), invertase. G-6PDH. Experience>2 years

Bioinformatics, Biostatistics and Mathematical Simulations.

Industrial expertization(Paper Work):

Planning & scheduling of batches & timely completion

Review of Technology Transfer Record

Preparation of Master documents for exhibit batches

Preparation of documentation in exhibit batches/ validation batches

Performing periodic discussions on protocols for updation

Leading the plant teams during Scale-up of new products to Mfg. scale.

Preparation of process validation documents and Scale up data

Data for regulatory submission

Investigation reports/ Trial reports

Train subordinates on GMP, process development & validation aspects

Ensuring GMP compliance and Continuous improvement of systems & SOPs

Implementation of new technologies through participation in discussions, training sessions

Education:

Jan,2006 Present Ph.D. Microbiology

Institution/University/Centre. Department Of Microbiology and Biotechnology Centre, (DST-FIST Sponsered), Maharaja Sayajirao University Of Baroda, Vododara, Gujarat,India.

Research Area/Topic Studies on Antibiotic biosynthesis by fluorescent Pseudomonas strains: Biochemistry and molecular Approach.

Ph.D Superviser Dr. G. Archana, Reader, (http://www.bcmsu.ac.in/archana.htm)

2004-2006 Job / Industrial exposure:

Company Intas Biopharmaceutical Ltd, Ahemedabad

Position/Rank/Post Hel Research Associate

Responsibility Protein purification of various therapeutic recombinant human proteins viz. Granulocyte colony stimulating factor (GCSF, PEG-GCSF), Erythropoietin, Interferon, Interleukin. Analysis of Purified Proteins.

2002-2004 M.Sc. (Biotechnology)

Institution/University/Centre. Biotechnology Centre, Department of Microbiology, M.S.University Of Baroda, Vadodara, Gujarat

Dissertation title: Random insertional mutagenesis of Magnaporthe grisea

(Blast fungus) to trace the gene(s) involved in their pathogenicity.

Dessertation Guide: Prof.B.B.Chattoo(http://www.bcmsu.ac.in/Bbc.htm)Director, Genome Research Centre., Co-ordinator Biotech Programme, M.S. Univ, Baroda

Fellowships / Awards:

FEMS - Young Scientist Meeting Grant from Federation of Euoropean Microbiological Society achieved in June ,2010

Best Poster Award at 1st Asian PGPR congress organized by Auburn University, USA and ANGRU, Hyderabad (21-24 June, 2009). (http://asiapgpr.freehostia.com/)

UGC Research Fellowship for Meritorious Students (2007-Present.)

Scholarship for the M.Sc Biotechnology Programme for the year 2002-2004 by Department of Biotechnology, Govt of India

Obtained 95 Percentile in Graduate Aptitude Test in Engineering (GATE-2004)

All India entrance exam for admission in Post Graduate in biotechnology ,conducted by JNU, New Delhi

Computational techniques:

Primer Design, Restriction Mapping, 3-D structure of proteins, Nucleotide and amino acid blast techniques, Evolutionary distant tree based on nucleotide sequence and restriction fragment length, real time primer design,2-D gel analysis.

Design of Experiment, Mathematical Modeling, Principal Component Analysis, Systat, Statistica, Mathmatica, Minitab, Matlab etc.

Memberships:

Biotech Research Society of India

Society of Biological Chemist of India

Association of Microbiologist of India

Symposium/ Training;/Conferences Presentations:

Abstracts:

Factors influencing polyketide, 2, 4-Diacetylphluoroglucinol (DAPG) production by rhizosphere competent fluorescent Pseudomonas strains: Presented at the 49th Annual International Conference by Association of Microbiologist of India at University of Delhi, New Delhi (18-20 Nov, 2008)

Combination of environmental factors influencing the physiology of bio-control in Pseudomonas fluorescensCHA0; Presented at the 77th Annual conference of Society of Biological Chemist at Indian Institute of Technology, CHENNAI (18-20 Dec,2008)

Best Poster Award out of 370 posters from India,USA,China,Indonesia,Holland and other countries across asia for the Work “Nutritional factors affecting the bio-control traits of fluorescent Pseudomonas in Vigna radiata rhizosphere”; Presented at the 1st Asian PGPR congress organized by Auburn University ,USA and ANGRU ,Hyderabad(21-24 June ,2009).

Studies on the interaction between bio-control strains of fluorescent Pseudomonas spp. and rhizobium spp. in the presence of fungal phytopathogen. Presented at International Workshop On Rhizosphere Biology of Agriculture, Horticulture & Forestry: Present & Future (February 25-27, 2010)

Training;

21 days hands on training on Novel and Innovative Biochemical and Molecular Tools for Characterization of Agriculturally Important Microorganisms at National Bureau of Agriculturally Important Microorganisms Kusmaur,Mau Nath Bhanjan,U.P.( Jan. 12 - Feb. 1, 2009)

Publications;

Molar fraction of carbon, nitrogen and mineral nutrients regulate the proportion of different antibiotics biosynthesis by Pseudomonas fluorescens CHA0. Chaubey Sumant, Talreja Shweta and G. Archana*. Manuscript under Preparation.

Sucrose and high inorganic phosphate does not inhibit the polyketide 2,4-DAPG production in the some fluorescent Pseudomonas isolates. Chaubey Sumant, Kotak Malini and G. Archana*. Manuscript under Preparation.

Role of organic acid and glucose dehydrogenase in Mineral rock phosphate solubilisation by Pseudomonas fluorescens CHA0. Chaubey Sumant,Dandekar Devdutt and G.Archana Manuscript Under Preparation

Education/Job Technical Exposure

Ph.D.(January,2006-Present) PCR (Degenerate, Nested PCR, Hot start, Inverse, Reverse Transcription-PCR and Q PCR), Gene Cloning, DGGE, 2-D Gel Electrophoresis, HPLC (Reverse Phase, Optimization of solvents, Quality of HPLC Columns Performance.

Research Associate(2004-2006) Purification of therapeutic proteins viz. G-CSF, Erythropoietin and IFN; Solubilisation of semi purified inclusion bodies, Refolding of misfolded /unfolded/intermediate proteins of inclusion body, ultrafilteration, size exclusion chromatography, ion exchange chromatography and analysis of purified protein by HPLC ,SDS PAGE,Western blotting, IEF.Animal cell culturing(CHO cell line) .TOC analyser(Shimadzu),Chromatography Column Packing.,

M.Sc.(Biotechnology) Genomic DNA isolation, Plasmid Isolation, Small RNA (5s rRNA, t-RNA etc) isolation, DNA, RNA and Protein electrophoresis, Transformation of bacteria and yeast, Polymerase chain Reaction, Study of E.coli Glucose-6-Phosphate Dehydrogenase. Mapping of gene markers by interrupted mating (conjugation), fine mapping by Transduction, Transposition. Isolation of the Auxotrophic strains of the chemically mutagenized Yeast. Isolation and Identification of the bacteria, Primary and Secondary Screening Techniques for isolation of industrially Important Bacteria

B.Sc.(Zoology) Drosophila culturing and maintenance,Monohybrid,Dihybrid and Trihybrid cross of Drosophilla genetics ,Ovarictomy of mice and mice maintenance.

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