Megan M. Bucher

h9qf48@r.postjobfree.com

570-***-****

*** ******* ******

Northumberland, PA 17857

Objective: Obtain a full time position as a laboratory technician to further my experience in research and development

Education:

Wilkes University Wilkes-Barre, PA

Bachelor of Science in Biology May 2009

Minor in Chemistry

GPA: 3.52/4.00

Dean’s List: Fall 2005, Fall 2007, Spring 2008, Fall 2008, Spring 2009

Senior Research:

Project Leader: Localization of TGF-B in Wild-Type and TSP-KO mice, Dr. Valerie Kalter

Work Experience:

Geisinger Health Services, Weis Center for Research

Genomics Core Research Technician August 2009-Present

•Lead data collection for gene mutation study of Polycythemia Vera by isolating DNA, amplifying via PCR, and organizing results

•Managed several DNA sequencing projects including Non-Alcoholic Steatohepatitis by collecting specimens, updating data, and storing samples

•Coordinated data entry for research projects using Microsoft Excel and Freezerworks applications

•Isolated DNA by using Qiagen robots M48 and Symphony Models

•Collaborated with scientists and staff to create future projects in partnership with research institutions

Wilkes University Biology Department

Intern/Research Assistant, Dr. Valerie Kalter Summer 2008

•Cultured lung fibroblasts both from wild-type and thrombospondin deficient mice

•Prepared histological slides of mouse brains in order to view anatomical differences between wild-type and thrombospondin deficient mice

•Updated lab mice conditions by changing cages, filling water and food, and monitoring activity

•Counted and recorded bacteria colonies from mouse colon on MacConkey agar

•Aided in RNA purification from mouse colon

Activities:

Sigma Alpha Pi, The National Leadership Honor Society 2008 - 2009

Beta Beta Beta, The Biological Honors Society, Historian 2007 - 2009

Wilkes University Women’s Tennis Team 2005 - 2008

Pre-Med Day Team, High School Program Volunteer 2007, 2008

Laboratory Techniques & Skills:

DNA Isolation

PCR

Immunohistochemistry

RNA Purification

Cell Culturing

Microscope Slide Preparation

Genotyping

Spectrophotometry

Data Management

Laboratory Machinery:

Qiagen M48

Qiagen Symphony

Nanodrop ND-1000 Spectrophotometer

Applied Biosystems Real-Time PCR 7500

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