Evgeny Makarev, Ph.D.

*** ****** ******* **** #** Gaithersburg MD 20878

+1-301-***-****

fn3xmt@r.postjobfree.com

Summary

. Excellent organization, presentation, and communication skills.

. Hands-on experience with NCBI databases with DNA/RNA sequence

analysis

. Expertize in Genomics, Proteomics, and Cell Signaling.

. Knowledge of relational databases and coding experience with C++ and

PERL

Experience and Research skills

. Research Associate (2010 - present time) University of the District of

Columbia, DC Modeling of NF-kB signaling pathway. (system biology of

cancer).

Developed a new model system for cancer signaling network.

Analyzed cancer related expression profiles.

Identified new protein as a negative modulator of NF-kB pathway.

Utilized: Quantitative analysis of protein expression, statistical

software, ELISA, Multi-color flow cytometry, Co-immunoprecipitation, SDS

PAGE, Western blotting, bacterial and mammalian protein expression, in

vitro transcription, affinity protein purification.

. Postdoctoral scientist (2007 - 2010) NIH, Bethesda, MD

Stem cells in small intestine.

Found potential marker of intestine stem cells.

Created conditional knock-out of Ldb1 protein.

Utilized: Confocal fluorescent live cell imaging, IF, electron microscopy,

rodent surgery, cell cultures, establishment primary cell cultures,

transfection, luciferase reporter assay, RNA interference, gel shift

assay, ELISA, multi-color immunochistochemistry, knock-out/transgenic mice,

mouse genotyping and maintenance mouse colony.

. Postdoctoral scientist (2005 - 2006) University of Dayton, Dayton, OH

miRNAs identification during newt's lens regeneration.

Established model system to study eye regeneration in

amphibians.

Developed new protocol for miRNA isolation.

Found differently expressed miRNAs in regenerating eye.

Utilized: RNA/DNA purification, cDNA libraries, RT-PCR, QPCR,

Northern/Southern blotting, in situ hybridization, molecular cloning,

mutagenesis, microarray-based technologies, histology, IHC, amphibian

surgery.

Education

. Ph.D., Developmental Biology, 2005

Koltzov Institute of Developmental Biology, Russian Academy of Science,

Moscow, Russia.

. M.Sc., Cell Biology, 1999

Dept. of Molecular Biology, Moscow State University, Moscow, Russia

Additional Courses (FAES graduate school Bethesda, MD)

. 2012 Theoretical and Applied Bioinformatics

. 2012 C++ Programming

. 2012 PERL Introduction

. 2009 Immunofluorescence and Confocal Microscopy

. 2009 Public Speaking

List of poster and oral presentations

1. Evgeny Makarev. CCDC130 protein is a cellular partner of Traf2 and a new

caspase8 substrate. AACR Annual Meeting 2012. Chicago, Illinois March 31-

April 4, 2012

2. Evgeny Makarev, Marat Gorivodsky and Heiner Westphal. Islet1 and its co-

factor Ldb1 express in quiescent cells of mouse intestinal epithelium.

(2009) NICHD 4th Annual Meeting.

3. Evgeny Makarev, Heiner Westphal. Islet1 and its cofactor Ldb1 express in

the mouse intestinal epithelium. (2008) Society for Developmental Biology

67th Annual Meeting. Abstract #293 published in Developmental Biology 319

(2008) 553-560.

4. Evgeny Makarev. Role of LIM-homeobox Genes in Adult Small Intestine.

(2007) NICHD Third Annual Meeting.

5. Makarev E.O. Expression of regulatory genes Pax6, Prox1, Six3 during

lens and retina regeneration in adult newts // Russian Journal of

Developmental Biology, Vol. 35, No. 5, 2004, pp. 320. (Abstract of Paper

Presented at the Summary Conference "Stem Cell Biology" (Moscow, January

28-29, 2004)

List of publications

1. E. Makarev and M. Gorividskiy. Islet1 and its co-factor Ldb1 are

expressed in quiescent cells of intestinal epithelium. Molecular and

Cellular Biology (in press)

2. P. A. Tsonis and E. Makarev. On dorsal/ventral-specific genes in the

iris during lens regeneration. // Cell. Mol. Life Sci. 65 (2008) 41 - 44

3. Evgeny Makarev, Mindy K. Call, Matthew W. Grogg, Donald L. Atkinson,

Brett Milash, Shannon J. Odelberg, Panagiotis A. Tsonis. Gene expression

signatures in the newt irises during lens regeneration. // FEBS Letters

581 (200*-****-****

4. Evgeny Makarev, Jason R. Spence, Katia Del Rio-Tsonis and Panagiotis A.

Tsonis. Identification of miRNAs and other small RNAs from the adult

newt eye. // Molecular Vision 12 (2006) 1386-91.

5. Markitantova Yu.V., Makarev E.O., Smirnova U.A., Zinov'eva R.D.,

Mitashov V.I. Analysis of the expression pattern of regulatory genes

Pax6, Prox1 and Six3 during regeneration of newt eye structures. //

Biology Bulletin, 2004, Vol. 31, No. 5, pp. 428-436.

6. Markitantova Yu.V., Makarev E.O., Pavlova G.B., Zinovieva R.D., Mitashov

V.I. Location of the Prox1 Gene Expression during Newt Lens and Retina

Regeneration // Doklady Biological Sciences, Vol. 391, 2003, pp. 361-

364.

7. Makarev E.O., Zinovieva R.D., Mitashov V.I. Expression of regulatory

homeobox genes during regeneration of retina in adult newts. // Biology

Bulletin, 2002, Vol. 29, No. 6, pp. 540-544.

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