10 years of experience in Research and Development

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Dr. Alok Kumar Tripathi, Post-Doctoral Fellow

Department of Biochemistry, Weizmann Institute of Science, Rehovot-76100, Israel.

(M) +918*********, +972-*********, Email: adjvc0@r.postjobfree.com, adjvc0@r.postjobfree.com Summary of Qualifications:

10 years of research experience in the field of chemistry and biology, synthetic and structural characterization of organic (drug) and inorganic molecules, synthesis and characterization of different types of polymers (degradable and non-degradable), catalysis (homogeneous and heterogeneous).

Surfactants (SDS, CTAB, Tween-20, Triton X) and bio-surfactants (bile salts, lipids, fatty acids), chemistry of soft matters (DNA, proteins), preparation of gel and its characterizations, chemistry of nanoparticles and nanomaterials, photobiology and chemical biology.

Spin chemistry (NMR and EPR), laser spectroscopy, spectroscopy (fluorescence and phosphorescence), XRD, microscopy, flow cytometry, Mass, MALDI, IR, GPC, HPLC, rheometer, chromatography. Research experience:

Post-Doctoral Fellow, Biochemistry lab, Weizmann Institute of Science, Israel (Jul. 2019- till now).

Isolation, purification of bacteriophages and their structural determination by TEM imaging.

Identification of pathogenic bacteria by using labeled bacteriophages by using plate-reader, flow cytometry, Flow imager and confocal microscopy.

Isolation and synthesis of polypeptides and their characterization by using Nano pores sensing.

Isolation of DNA or RNA and their identification by using Nano pore sensing and PCR. Post-Doctoral Fellow, Synthesis and Photochemistry lab, NTU-Taiwan (Jan. 2019-Jul. 2019).

Design and multi-steps synthesis of two color emitter fluorescent molecules (drug) and their structural characterization by NMR (1H and 13C), IR and mass spectrometry.

Photophysical and self-aggregation behavior of these drug molecules in different solvent systems. Characterization of nanostructures or aggregates by UV-Visible, fluorescence spectroscopy, DLS, zeta potential measurement, SEM and TEM techniques.

Application of fluorescent drug molecules on treatment of MF7-cells by using fluorescence microscopy. Post-Doctoral Fellow, Synthesis, Photochemistry and EPR lab, TIFR-Mumbai (Jan.2017-Jan. 2019).

Synthesis of TEMPO (free radical) containing naphthalene, anthracene, pyrene and anthraquinone) molecules and their structural characterizations by NMR (1H and 13C), IR, Mass spectrometry.

Distance, solvent and concentration dependent photophysical quenching of excited singlet and triplet states of the chromophores by the linked TEMPO by using steady-state and time-resolved EPR experiments, nanosecond flash photolysis instrument and TCSPC.

Distance, concentration and solvent dependent, formation of charge transfer state in the chromophore covalently linked with TEMPO by using steady-state and time-resolved fluorescence. Research Associate, Catalysis and Polymer lab, CSIR-NCL-Pune (Jun 2016-Jan. 2017).

The title of the project “Exploratory research on the development of homogeneous catalysts for copolymerization of CO2 with propylene oxide”.

Industry sponsored - Expanded Polymer Systems Pvt Ltd, C-44/1 & 2, C-45/3, T.T.C. Area, MIDC, Pawane Village, Navi Mumbai – 400705, Maharashtra, India.

Synthesis of Schiff base ligands and their Zn2+, Co3+ and Cr3+ catalysts in bulk quantity.

Structural characterization by using NMR (1H and 13C), IR, mass spectrometry and X-ray crystallography techniques.

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Catalytic copolymerization of epoxides and CO2 for production polycarbonates and cyclic carbonates in bulk scale. Poly-carbonates were characterized by NMR (1H and 13C), FTIR, GPC, MALDI. Ph.D., Synthesis and Photochemistry lab, at IIT-Madras, Chennai (Jan 2011-June 2016).

Synthesis of fatty acid containing dansylamide, 1-naphthol and acridine based fluorescent drug molecules and their structural characterization by using NMR (1H and 13C), IR and Mass spectrometry.

Basic photophysics like quantum yield, solvent study, polarity scale, anisotropy, by using steady-state time-resolved fluorescence instruments.

Micellization process of surfactants (SDS, CTAB, Triton X-100 and Tween-20), formation of mixed micelles with bile salts (NaDC and NaC) and cholesterol for fat salubilizaton by using steady-stat and time resolved fluorescence instruments.

Structure and dynamics of lipid bilayer membrane (constituents for cell membrane and a drug carrier) and Niosomes (a drug carrier in biological systems).

Binding mechanism or host-guest relation between a drug carrier like cyclodextrine or ct-DNA with these drug by using steady-state, time-resolved fluorescence, DSC and CD experiments. Research Assistant, Organometallic and Polymer lab, at IIT Madras, Chennai (Jan. 2008- Jan. 2011).

Design and synthesis and structural characterization (1H and 13C NMR, IR, Mass) of polydentate ligands

(Mannich base or Schiff base) and their azeotropic distillation to remove moisture.

Synthesis of mono- and bi-nuclear metal complexes of Zn2+ and Sm3+ in glovebox, under dry conditions by using ppm level moisture and oxygen free dry solvents. Structural characterization using NMR (1H and 13C), IR, Mass spectrometry and X-ray crystallography.

Production of biodegradable polylactides and polylactones by catalytic polymerization. Biodegradable polymers were characterized by NMR (1H and 13C), IR, FTIR, GPC, and MALDI. Educational Qualifications:

B.Sc. in 2005 from D. D. U. Gorakhpur University in Chemistry, Botany Zoology (1st).

M.Sc. in 2007 from D. D. U. Gorakhpur University in Chemistry (1st).

Ph.D. in 2016 from IIT-Madras, Chennai in Organic and Physical Chemistry. Technical skills:

Synthetic skills: Organic molecules (drug), organometallic compounds (Zn2+, Co3+, Fe3+, Sm3+, Cr3+, Ti4+, Hf4+, Zr4+), catalysts, nanomaterials (nanoparticles), organic and inorganic photovoltaic materials and semiconductors, biodegradable polymers (polylactides, polylactone, polycarbonates), non-bio- degradable polymers (polyethylene), synthesis and characterization of gel, high pressure gas polymerization reaction in Parr-reactor.

Characterization and purification techniques: Bruker and Varian NMR (400, 500, 600, 700 and 800MHz), IR, FTIR and Raman spectroscopy, mass spectrometry, GCMC, HRMS, MALDI, GPC, HPLC, chromatography, TLC, Rheology, powder XRD.

Handling of biological materials: different type of bacteria, viruses, protein, DNA, MF7 cells, yeast.

Moisture and oxygen sensitive reagent handling: Glove box, pyrophoric substances like butyl lithium, Grignard reagent, LiAlH4, Gilman reagent and other metal salts, handling of Na and K metal.

Steady-state spectrometers: Bruker-EPR instrument, PerkinLambda and JASCO UV/vis spectrometer, Fluorolog-3 and FluoroMax-4 fluorimeter, Netzsch-DSC instrument, Jasco CD spectrometer, Malvern Zetasizer-DLS instrument, Biotek and Tecan microplate reader, NanoDrop spectrometer.

Time-resolved spectrometers: Laboratory-built EPR spectrometer, Laboratory-built setup picosecond laser or nano-LED based TCSPC-lifetime instrument, Nanosecond laser flash photolysis spectrometer. 3

Laser used: Excimer laser, Nd:YAG laser, dye laser, tsunami laser (10W and 5W).

Imaging instrumental Skills: Flow cytometry and imageStreamX, Zeiss and Nikon fluorescence microscope, Olympus and Leica confocal microscopy, SEM, TEM techniques.

Software: Origin, Mercury, Topspin, Chem-draw Software. Publications in journals (total 9):

Alok Kumar Tripathi, M. Mohapatra and A. K. Mishra, Fluorescence of N-acylated dansylamide with a long hydrophobic tail: sensitive response to premicellar aggregation of sodium deoxycholate. Phys. Chem. Chem. Phys., 2015, 17, 299**-*****.

Alok Kumar Tripathi, Binding interaction of N-acylated dansylamide derivatives with β-cyclodextrin and DPPC lipid bilayer membrane:A fluorescence study. J.Fluoresc., 2018, 28, 409-417.

Alok Kumar Tripathi, Binding interaction of N-acetylated acridine conjugate with ct-DNA and β- cyclodextrin: Synthesis and photophysical studies. Spectrochim. Acta A, 2018, 205, 497–502.

Alok Kumar Tripathi, Interaction of fatty acid containing 9-aminoacridine derivative with surfactants and bio-surfactants: Synthesis and photophysical studies. J. Photochem. Photobiol. A 2018, 367, 321.

Alok Kumar Tripathi, S. Kundu and R. Das, Distance-dependent formation of electronic charge- transfer states in the ground states of anthracene and pyrene covalently linked to a TEMPO free radical. Phys. Chem. Chem. Phys., 2019, 21, 77-88.

Alok Tripathi and Vinayak Rane, Towards achieving the theoretical limit of electron spin polarization in covalently linked radical-chromophore dyads. J.Phys.Chem.B, 2019, 123, 6830−6841.

Alok Kumar Tripathi, V. Rane, S. Kundu, and R. Das, A phenomenological scheme for reversed- quartet mechanism of electron spin polarization in covalently linked systems of chromophore and free radical: determination of magnitude of polarization and application to pyrene-TEMPO linked molecules. J. Chem. Phys., 2019, 151, 154305.

Alok Kumar Tripathi, Interaction of alkyl chain containing pyrene derivatives with different micellar media: Synthesis and photophysical study. Spectrochim. Acta A, 2020, 228, 117806.

P.Mani, A.K.Pandey, Alok Kumar Tripathi, Synthesis, Binding Affinity of Hydrophobic Tail Containing Naphthalene Derivatives with Different Type of Organized Media. ChemistrySelect, 2020,5, 2135. Conferences and workshops: (total 10)

Alok Kumar Tripathi and R. Das (2018) 14th DAE-BRNS, TSRP, BARC, Mumbai, India. Jan. 2018.

Alok Kumar Tripathi and A. K. Mishra (2015) MAF-14, Würzburg, Germany, September 2015.

Alok Kumar Tripathi and A. K. Mishra (2014) 8th APC -Trivandrum, Kerala, India. Nov.2014.

Alok Kumar Tripathi attended the workshop on colloids and interfaces with polymers and surfactants, Indian Institute of Technology, Madras, from 3 to 7 March 2014. Awards, Scholarships and Fellowships (total 6):

Marcus Postdoctoral Fellowships in Water Sciences - Fall 2019, Ben-Gurion University of the Negev, Beer Sheva, 84105.

Junior and senior research fellow of CSIR, New Delhi, India: December 2010 and June 2010.

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