Degree

Year

Field/School

Class/ Division

University/Board

Postdoc

****-****

Department of Biochemistry

University of California, Riverside, USA.

Ph. D.

2010

Department of Biotechnology

National Centre For Cell Science, University of Pune, Maharashtra, India

M. Sc.

2003-2005

Department of Biochemistry

First

University of Delhi, India,

B.Sc.

2000-2003

Department of Biochemistry

First

University of Delhi, India,

Honors and relevant experience

2014 Postdoctoral fellow at National Institute of Health, Bethesda, MD

2013 Senior resident at Vardhman Mahavir Medical College, New Delhi, India

2011-2012 Postdoctoral fellow at University of California, Riverside, USA

2007-2010 Senior Research Fellowship, National Centre for Cell Science

(NCCS), India

2005-2007 Junior Research Fellowship, National Centre for Cell Science

(NCCS), India

Personal Statement

I am highly motivated researcher to carry out research in the field of cancer biology. I have a

broad background in cancer biology with specific training and expertise in cell and molecular biology. As a doctoral fellow at NCCS, Pune, India, I played the key role in completing few projects. More specifically, using molecular biology tools, I have demonstrated the transcriptional regulation of osteopontin gene in cervical cancer model.

In addition, I successfully worked on cancer drug discovery program using target specific

synthetic drug for cervical cancer. I have excellent expertise in various techniques such as confocal microscopy, immunohistochemistry, Electrophoretic Mobility Shift Assay (EMSA), western blotting, gene and promoter cloning, protein expression, RT-PCR and mice experiments. My sincere efforts and scientific attitude prompted me to produce several peer-reviewed international publications. In summary, I have demonstrated testimony of accomplished and productive research in an area of cancer biology, and my expertise and experience have prepared me a suitable candidate for this position.

Chromatin biology,

Epigenetics,

Signal transduction,

Cancer Biology,

Tumor Metastasis,

Angiogenesis

1. “Investigation of in vitro activation of ERK 1 and 2 upon membrane fusion” under the supervision of Prof. Debi P. Sarkar, Department of Biochemistry, University of Delhi.

2. “Designing of vectors for expression of bacteriophge λ ‘D’ protein based fusion proteins” under the supervision of Dr. Vijay K. Choudhary, Department of Biochemistry, University of Delhi.

• Cell Culture

1. Mammalian cell culture.

2. Primary culture of Mouse Embryonic Fibroblast (MEF) Cells.

3. Hypoxic culture of cells.

• SDS Gel Electrophoresis, Western Blot, Immunoprecipitation.

• Gelatin, Casein and Plasminogen Zymography.

• Isolation of DNA and RNA, Primer designing, RT-PCR, DNA agarose gel electrophoresis.

• Protein purification and quantification of protein.

• Bacteria Culture and Purification of Plasmid.

• Transfection of cell lines and production of novel cell lines with specific genetic characteristics (generation of stable clones).

• Cloning and expression of gene.

Co-Immunoprecipitation

• Chromatin Immunoprecipitation (ChIP) assay.

• siRNA design and transfection.

• Immunofluoresence study, confocal microscopy, and flow cytometry.

• Cell viability, proliferation, migration, co-migration, invasion, colonigenecity, adhesion,

tumor stromal and tumor endothelial interaction assays.

• Electrophoresis mobility shift assay (EMSA).

• Kinase assay and autophosphorylation study.

• Angiogenesis assays like in-vitro tube formation assay, in vivo matrigel migration assay,

• Animal related work,

1. Generation of xenograft cervical and melanoma and other tumors in nude mice.

2.Isolation of blood from recto-orbital plexus.

3.In vivo invasion assay.

4.Colony maintenance

• Human clinical sample analysis.

• Histopathology and immunohistochemical study of mice and human tumor samples and

Analysis of clinical samples.

1.Kumar S, Sharma P, Kumar D, Chakraborty G, Gorain M, Kundu GC. Functional characterization of stromal osteopontin in melanoma progression and metastasis PLoS One. 2013 Jul 23;8(7):e69116. doi: 10.1371 Print 2013.

2.Santosh Kumar, Priyanka Sharma, Harshal Patil, Dheeraj Kumar, H thulasiram and Gopal C. Kundu. Andrographolide Inhibits Osteopontin Expression and Breast Tumor Growth Through Down Regulation of PI3 kinase/Akt Signaling Pathway. Curr Mol Med 2012 (paper in press)

3.Muyu Xu, Priyanka Sharma, Songqin Pan, Sohail Malik, Robert Roeder, Martinez E. Core promoter-selective function of HMGA1 and Mediator in Initiator-dependent transcription. Genes and Development, 25(23): 2513-24, 2011.

4.Ahmed M, Behera R, Chakraborty G, Jain S, Kumar V, Sharma P, Bulbule A, Kale S, Kumar S, Mishra R, Raja R, Saraswati S, Kaur R, Soundararajan G, Kumar D, Thorat D, Sanyal M, Ramdasi A, Ghosh P, Kundu GC. Osteopontin: a potentially important therapeutic target in cancer. Expert Opin Ther Targets. 2011 Sep;15(9):1113-26. Jul 25.

5.Priyanka Sharma, Santosh Kumar and Gopal C. Kundu. Transcriptional regulation of human osteopontin promoter by histone deacetylase inhibitor, trichostatin A in cervical cancer cells. Molecular Cancer, July 7, 9 (1), 178, 2010.

6.Saurabh J Pradhan, Rosalin Mishra, Priyanka Sharma and Gopal C. Kundu. Quercetin and sulforaphane in combination suppress the progression of melanoma through the down-regulation of matrix metalloproteinase-9. Experimental and therapeutic medicine. 2010 Nov;1(6):915-920. Epub 2010 Aug 26.

7.Goutam Chakraborty, Shalini Jain, Reeti Behera, Mansoor Ahmed, Priyanka Sharma, Vinit Kumar and Gopal C. Kundu. The Multifaceted Roles of Osteopontin in Cell Signaling, Tumor Progression and Angiogenesis. Curr Mol Med, 6: 819-830, 2006.

8.Kumar S, Sharma P, Kundu GC. Endocytotic mediated nuclear localization of osteopontin and its role in cell cycle progression in cancer cell (manuscript under preparation).

9.Sharma P, Kumar S, Kundu GC. A novel compound CH-330 inhibits osteopontin expression and suppresses in vivo cervical tumor growth (manuscript under preparation).

1.Oral presentation in 3rd International Symposium on Translational cancer research: cell signalling and cancer therapy held in Bhubaneswar from December 18-21, 2009.

2.Poster entitled “Transcriptional regulation of osteopontin (OPN) by trichostatin A (TSA), a Histone deacetylase inhibitor” was presented in “International symposium on Novel strategies for targeted prevention and treatment of cancer” held in JNU Delhi from December 19-20, 2008.

3. Participated in 30th All India Cell Biology Conference & Symposium on “Molecules to Compartments: Cross-Talks and Networks” held in New Delhi from February 2-4, 2007.

4.Participated in 10th Asian conference on Transcription and Transcription assembly held in Bangalore from January 13-16, 2008.

Prof Debi P Sarkar,

Department of Biochemistry

Address (Campus) University of Delhi South Campus, New Delhi-

110021

(Residence) Type VB, Flat No. 6A, Ground Floor, UDSC,

New Delhi-110021, INDIA

Phone No (Campus) 91-11-241*****, 981*******

Fax 24115270, 24110283

Email adj9ge@r.postjobfree.com

Ernest Martinez, Ph.D.

Associate Professor

Department of Biochemistry - Boyce Hall 4434

University of California Riverside

900 University Ave.

Riverside, CA 92521-0127

Tel. : 951-***-**** (Office)

951-***-**** (Lab)

Fax : 951-***-****

E-mail: adj9ge@r.postjobfree.com

http://biochem.ucr.edu/faculty/martinez/martinez.html

Dr Vasudevan Sheshadri

National Centre For Cell Science, Pune NCCS

Department of Cell Biology

Email adj9ge@r.postjobfree.com

Priyanka Kumar, Ph.D

1001 Rockville Pike,

Apt#1620

Rockville, Maryland

20852

Mobile: +1-240-***-****

E-mail: adj9ge@r.postjobfree.com

Educational Background

RESEARCH INTERESTS

PROJECTS DONE DURING MASTERS DEGREE

EXPERIMENTAL EXPERTISE

PAPERS PRESENTATION IN CONFERENCES.

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Selected peer reviewed publications

CONFERENCE PRESENTATIONS

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REFERENCES/RECOMMENDATIONS

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