Curriculum Vitae

Dr Agnes Abiola Oluwatoyin Awomoyi, PhD.

***** ********* **., ********** ******** US 20184

Email: adcm5p@r.postjobfree.com or adcm5p@r.postjobfree.com. cell phone: 540-***-****

● Educational History:

Diploma in Nursing and Patient Care ALISON online Education May 2018 Diploma in Nursing Leadership and Care Management ALISON online Education May 2018 Diploma in Clinical Nursing Skills ALISON online Education May 2018 Bachelor of Science in Microbiology General (July 1987) from Faculty of Science, Biological Sciences Department, Akoka Campus, University of Lagos, Nigeria. Dissertation: The use of an evaporative cooler or ambient temperature for storage of tomato, Lycopersicum esculentum var miller.

Master of Science in Medical Microbiology (1989) from The College of Medicine of the University of Lagos Idi-Araba, Surulere, Lagos, Nigeria. Dissertation: Study of sexually transmitted diseases in Lagos Nigeria.

Doctor of Philosophy in Human Genetics (Nov 15th 2000) from The Open University U.K. Walton Hall Milton Keynes MK7 6AA United Kingdom. Thesis title: Genetics of susceptibility to tuberculosis.

● Professional Experience:

Adjunct Faculty (08/2018 - Present)Northern Virginia Community College (NVCC) Sterling Loudoun County Virginia. I teach General Biology BIO 101 and BIO 102 (lecture and laboratory Instructor).

Senior Researcher/Grant Writer (April 2012-2017) Restorative Therapeutic Services In charge of logistics and all research tasks related to the needs of the company. Researched and applied for a grant for the company. Managed office personnel and directed clients to prospective clinicians Trained office employees to ensure that all necessary information were easily accessible. Acted as the client clinician liaison.

Principal Investigator: (2010 – 2012). AbiBi01, LLC: 1) Determination of the mechanisms by which J2 virus immortalized murine and human cells: a model for understanding emerging human diseases of murine virus origin and how SLC11A1 links murine virus infections with the development of human malignancies especially leukemias. 2) Epigenomic, genomic and exogenous determinants of gene expression during monocyte differentiation. Skills and Expertise: I make my own PCR buffers and Taq DNA polymerase especially for high GC and genomic regions rich in repeat elements. I design my own PCR primers, shRNAs and siRNAs with near 100% accuracy. I create my own mammalian expression vectors whether it be promoter region, full length or fusion gene. I transform primary innate immune cells such as macrophages and Dendritic cells to immortalized cell-lines. I have use viral oncogenic constructs to transform human and murine PBMCs. I have worked with animal gene knock out models. I perform microsurgery to harvest cells and organs from murine animal models. Now, I would like to use newer technology which utilizes gene editing and avoids genome instability to create gene knockout in human and murine mammalian cells. This is especially important for accurately teasing out the particular role of a gene in oncogenic processes. I isolate, engineer and re-engineer vesicles which originate from the endo-lysosomal pathway and interact with the nucleus. Patent Principal Researcher: (2010 – Present). Article One Partners. I research prior acts in the Life Sciences discipline especially in the areas of Biotechnology and Molecular Biology for inclusion in a said patent application prior to filing with the USPTO. In addition, I specifically search for areas or claims within a said patent application which conflict with previous works whether published or not; to avoid potential lawsuit for the present patent application. This is part time position. 555 Madison Ave 30th fl, New York NY 10022 WyzAnt Tutoring: (2011 -Present). Immunology, Biology and Genetics. I provide home schooling or tutoring in aforementioned subjects to a wide range of students including high school students, who are preparing for the AP Biology exams. In addition, I provide tutoring to undergraduate and graduate students who wish to gain a better understanding or grounding of difficult concepts in Biology especially Genetics, Organismal Biology and Microbiology. This is a part time position. http://www.wyzant.com/Tutors/MD/Bethesda/7938398/#ref=1RJRFA Postdoctoral Fellow/ Principal Investigator: (2008 -2010). Postdoctoral Research Fellow in Inflammation: Proteins of the lysosomal pathway, the inflammasome and pathology. Dorothy Davis Heart and Lung Institute. Ohio State University Medical Center (DHLRI-OSUMC) Columbus OH 43210. USA.

T32 Postdoctoral fellowship Advisor: Professor Mark D. Wewers Postdoctoral Fellow training in Cell signaling with secondary assignment in Human Genetics of susceptibility to Respiratory Syncytial Virus diseases in young infants (2003-2007) at Department of Microbiology & Immunology, University of Maryland School of Medicine Baltimore, MD. Human Genetics: Genetics of susceptibility to Respiratory Syncytial Virus. Cell signaling: Intracellular signaling leading to the induction of genes that are involved in macrophage activation. Advisor: Professor Stefanie Nancy Vogel Faculty Research Assistant (2001-2003) Department of Biology, University of Maryland, College Park MD 20742 USA. Human Genetics: Genetics of susceptibility to infectious diseases and relevance to human evolution. Microsatellite and sequence variation within and around SLC11A1 gene in globally diverse human populations. Field study in Nigeria: Organization, recruitment of subjects and collection of blood samples for population genetics study. Advisor: Professor Sarah Anne Tishkoff

Higher Scientific Officer/Open University UK, Graduate Student (1995-2000) Medical Research Council Laboratory Fajara, P. O. Box 273 Banjul, The Gambia. Virology: Epidemiology of RSV in The Gambia. Respiratory Syncytial Virus (RSV) culture and isolation. Detection of RSV by immunofluorescence. Detection of C. pneumoniae by nested Polymerase Chain Reaction (PCR) from nasopharyngeal and lung aspirates of Gambian children. Micro-neutralization assay, maintenance and growth of HEP-2 cell lines. Production of EBV transformed B cell lines. Cryo-preservation of B cell lines in liquid nitrogen phase. Immunology: - Whole Blood assays, ELISPOT assays for detection of intracellular cytokine. Cytokine ELISA for the detection of released cytokines, Detection of cell surface molecules by FACS analysis. Molecular Biology: - DNA extraction, PCR RFLP, ARMS PCR, Design of PCR primers, Optimization of PCR amplification conditions. Genetic analysis: Genetics of susceptibility to tuberculosis in humans. Genetics of susceptibility to meningitis in the Gambia. Gambian Twins study. Skills acquired: DNA sequencing by both manual and semi- automated techniques (Big dye primer and dye terminator chemistry). Microsatellite analysis by both manual and semi-automated techniques. Graduate School Advisors1) Professors Keith PWJ McAdam, Melanie Jane Newport and Jennie Margaret Blackwell. Visiting Research Assistant/Student Intern (1992-1993) Institution: ARC Epidemiology Research Unit, University of Manchester, Stopford Building Manchester M13 9PT UK. Tissue culture;Tissue typing, Production of EBV transformed B cell-lines and cryopreservation in liquid nitrogen phase. Molecular Biology: Oligonucleotide synthesis by phorsphoramidite chemistry. Allele specific oligonucleotide hybridisation of HLA DRB* ARMS PCR of Transporters Associated with Antigen Presentations (TAP) gene polymorphisms. Sequence specific oligonucleotide analysis of HLA DPB*. Professor William (Bill) Ollier Junior Research Fellow (1991-2001) Institution: Immunogenetics Unit, Nigerian Institute for Medical Research Yaba. Lagos. Nigeria. Field study: - Blood sample collection from leprosaria within Nigeria. Laboratory work: DNA extraction for genetic analysis (Leprosy). Complement factor B allotypes in HIV infections. Serodiagnosis of HIV. Investigation into genetic factors which may determine susceptibility into different clinical manifestations seen in leprosy patients in Nigeria.

● Brief Research Interest: My ultimate research goal is to design and carryout a study that should contribute to the understanding of the mechanisms by which a balance is maintained between infections caused by pathogens (ranging from viral, bacterial and protozoan; intracellular vs. extracellular) and disorders resulting from (acute vs. chronic) inflammation by studying host/ pathogen interactions that determines how the sterile cellular stress, infections lead to neurodegenerative disorders. In addition, I would like to gain a comprehensive understanding of the interactions of the imaging, biological and signaling networks invoked by the host that result in either susceptibility or resistance to infections, neurodegenerative disorders.

● Professional Memberships:

1. The American Society of Human Genetics. (ASHG)

2. Society of Leukocyte Biology (SLB)

3. International Endotoxin and Innate Immunity Society (IEIIS)

● Honors and other Recognitions:

1. British council’s CHEVRON SCHOLARSHIP 1992-1993 (formerly called Foreign and commonwealth office fellowship, British Council Office Lagos Nigeria for a research attachment at the ARC Epidemiology Research Unit University of Manchester UK). 2. First British Society for Histocompatibility and Immunogenetics Travel Award 1998-1999.

3. Annual Short Course on Medical and Experimental Mammalian Genetics Travel award 2005.

4. FASEB travel Awards (severals)

Original Achievements, Investigations and Research.

● Completed GRANTS

● World Health Organization Medical Research Council Laboratories Banjul The Gambia: - Grant no 95012 Detection of Chlamydiae by nPCR in nasopharyngeal and lung aspirates of malnourished Gambian children with or without pneumonia 1997-1998. $12,000

● The Sir Halley Stewart Trust Medical Research Council Laboratories Banjul The Gambia and University of Cambridge Addenbrookes. Cambridge United Kingdom: - Who gets Tuberculosis? 1997-2000. £52500.

● Online citations

● Wikipedia citations and references under the following titles: 1. SLC11A1 further reading section

2. Sub-Saharan Africans,

3. Bushmen,

4. History of Africans

● Mendelian Disorder in Man (OMIM)

1. +603030 Toll-like Receptor-4 TLR-4

● other online citation

1. CITATIONhttp://www.membranetransport.org/protein.php?pOID=hsap2&pSynonym= NP_000569

● Commercial Company citation

1. anti-SLC11A1 polyclonal antibody (Human). MBL medical and Biological Laboratories co Ltd URL https://ruo.mbl.co.jp email adcm5p@r.postjobfree.com Tel 052***-****

● Books:

1. How can we use Natural variation in disease resistance to understand host pathogen interactions and devise new therapies. Working Group 14b. The Genomic Revolution: Implications for treatment and control of infectious disease. The 3rd National Academies Keck Futures Initiative, Irvine CA 2006

● Serve as peer reviewer for the following:

1. Thorax; an International Journal of Respiratory Medicine, 2. The International Journal of Tuberculosis and Lung Disease (IJTLD), 3. Immunogenetics and the International Journal of Biomedical Sciences. 4. Society of Leucocyte Biology’s Journal of Leucocyte Biology.

● Peer-reviewed primary publications:

1. Awomoyi A. A. O, Donn R P, Davies E J, Carthy D, Thomson W, Ollier W E R. 1995 Absence of TAP 2D in Yoruba Nigerians. Eur J Immunogen 22,187-190. 2. Carthy D, Macgregor A, Awomoyi A, Rigby A S, Thomson W, Donn R, Silman A, Ollier WER. (1995) HLA-DPB1*0201 is associated with particular clinical features of rheumatoid arthritis. Rev. Rheum. [Engl. Ed.], 62 (3) 163-168. 3. Koch O, Awomoyi A, Usen S, Richardson A, Hull J, Pinder M, Newport M, Kwiatkowski D. (2002) IFNGR1 promoter polymorphisms and susceptibility to cerebral malaria. J Infect Dis. Jun 1;185(11):1684-7.

4. Awomoyi A A, Marchant A, Howson JMM, McAdam, Blackwell JM, Newport MJ. (2002) Interleukin-10, Polymorphism in SLC11A1 (formerly NRAMP1) and Susceptibility to Tuberculosis. J Infect Dis. Dec15 186: 1808-14.

5. Weber MW, Milligan P, Sanneh M, Awomoyi A, Dakour R, Schneider G, Palmer A, Jallow M, Oparagou A, Whittle H, Mulholland EK, Greenwood BM. An epidemiological study of RSV infection in the Gambia. Bull World Health Org. 2002; 80: 562-568. 6. Newport JM, Awomoyi AA, Blackwell JM. Polymorphism in interferon gamma receptor- 1 gene and susceptibility to tuberculosis in The Gambia. Scandinavian J of Immunol. 2003 58; 383-385.

7. Allen A, Obaro S, Sirugo G Awomoyi A, Bojang K, Greenwood BM, Whittle H, Newport M. Variation in TLR4 and susceptibility to group A meningococcal meningitis in Gambian children. The Ped. Infect. Dis. J. Nov 2003 22; 11 8. Awomoyi A, Nejentsev S, Richardson A, Hull J, Koch O, Todd JA, KPWJ McAdam, Blackwell J, Kwiatkowski D, Newport MJ. Polymorphism in interferon gamma receptor 1

(IFNGR1) and susceptibility to tuberculosis Thorax 2004; 59: 291-294. 9. Newport MJ, Allen A, Awomoyi A, Dunstan S, Sirugo G. The toll-like receptor 4 Asp299Gly variant : no influence on LPS responsiveness or susceptibility to pulmonary tuberculosis in The Gambia Tuberculosis (Edinb) 2004;84(6):347-352 10. Stockton JC,, Howson JM, Awomoyi AA McAdam KPWJ, Blackwell JM, Newport MJ. Polymorphism in NOD2, Crohn’s disease and susceptibility to tuberculosis FEMS Immunol Med Microbiol. 2004 1;41(2): 157-160

11. Medvedev AE, Thomas K, Awomoyi A, Kuhns DB, Gallin JI, Li X, Vogel SN. Cutting Edge: Expression of IL-1 Receptor-Associated Kinase-4 (IRAK-4) Proteins with Mutations Identified in a Patient with Recurrent Bacterial Infections Alters Normal IRAK-4 Interaction with Components of the IL-1 Receptor Complex. J.of Immunol. 2005 Jun 1;174(11):6587-91.

12. Awomoyi AA, Charurat M, Marchant A, Miller EN, Blackwell JM, McAdam KPWJ, Newport MJ. Interleukin (IL) – 1 -511 Polymorphism: Associated with tuberculosis and decreased LPS – induced IL-1 production after IFN- priming in a Gambian Population sample. J. of Endotoxin Res. 2005 11(5) 281-286

13. Vogel SN, Awomoyi AA, Rahllabandi P, Medvedev AE. Mutations in TLR4 signaling that lead to increased susceptibility to infection in humans: An overview J. of Endotoxin Res 2005 11 (6) 333-339.

14. Awomoyi A, Sirugo G, Newport J.M, Tishkoff S. Global distribution of a novel trinucleotide microsatellite polymorphism (ATA)n in intron 8 of the SLC11A1 gene and susceptibility to pulmonary tuberculosis. Int. J. Immunogenet. 2006 33, 11-15. 15. Weber MW, Gopalakrishna G, Awomoyi A,, Cunningham A, Adegbola RA, Falade AG, Ogunlesi OO, Whittle HC, Mulholland EK. The role of Chlamydia pneumoniae in acute respiratory tract infections in young children in The Gambia, West Africa. Ann. Tropical Paed. Int. Child Health. 2006 26, 87 -94(8).

16. Awomoyi AA. The human solute carrier family 11 member 1 protein: linking infections, autoimmunity and cancers? Mini Review FEMS Immunol Med Microbiol. 2007 49, 324-330.

17. Awomoyi AA, Rallabhandi P, Pollin T I, Lorenz E, Sztein M, Boukhvalova M, Hemming V, Blanco JGC, and Vogel SN. Association of Toll-like receptor 4 (TLR4) Extracellular Polymorphisms with Severe Respiratory Syncytial Virus (RSV) Infection in Infants and Young Children. J. Immunology 2007 Sep 1; 179(5): 3171-3177. 18. Rallabhandi P, Awomoyi AA, Thomas KE, Phalipon A, Fujimoto Y, Fukase K, Kusumoto S, Sztein MB and Vogel SN. Differential Activation of Human TLR4 Polymorphisms on Signaling Awomoyi AA J Immunology 2008 Jan 15 180 (2) 1139-1147. 19. Tishkoff SA, Reed FA, Friedlaender FR, Ehret C, Ranciaro A, Fremont A, Hibro J, Awomoyi AA, Bodo J-M, Doumba O, Ibrahim M, Juma AT, Kotze MJ, Lema G, Jason H. Moore, JH, Mortensen, H, Nyambo, TB, Omar SA, Powell K, Pretorius, GS, Smith, MW, Thera, MA, Wambebe C, Weber JL, and Williams SM. The Genetic Structure and History of Africans and African Americans. Sciencexpress Research Article. 2009 April 30th . 20. Tishkoff SA, Reed FA, Friedlaender FR, Ehret C, Ranciaro A, Fremont A, Hibro J, Awomoyi AA, Bodo J-M, Doumba O, Ibrahim M, Juma AT, Kotze MJ, Lema G, Jason H. Moore, JH, Mortensen, H, Nyambo, TB, Omar SA, Powell K, Pretorius, GS, Smith, MW, Thera, MA, Wambebe C, Weber JL, and Williams SM. The Genetic Structure and History of Africans and African Americans. Science Research Article. 2009 May 1st. 21. Awomoyi AA, Tishkoff SA. Microsatellite haplotype variability, extent of linkage disequilibrium and association studies at the SLC11A1 locus in globally diverse human populations. Manuscript with the senior author.

● Other publications:

1. Abstract: - Complement factor B allotypes and protein concentration patterns in HIV infections Uko G., Harry T., Awomoyi AAO. VIII international conference on AIDS/III STD World Congress Abstract Vol. Congrex Holland B.V.1992 2. Abstract: - Immunoserology of Nigerians with HIV infection UKO GP, Harry A, Awomoyi A. Nig. Soc. Immunol. Book of Abstract 1992.

3. Abstract :- Allelic mRNA expression imbalance in inflammasome related genes. AA Awomoyi, A Papp, G Smith, W Sadee M Wewers. Keystone Symposia Pattern Recognition Molecules and Immune Sensors of Pathogen Banff AB March 2009

4. Abstract :- Allelic mRNA expression imbalance in inflammasome related genes. AA Awomoyi, A Papp, G Smith, W Sadee M Wewers. The American Society of Human Genetics 59th Annual meeting Honolulu Hawai’i Oct 20-24th 2009.

● Invited Talks and Participations:

1. There is more to the Solute Carrier 11 family 11 member 1 (SLC11A1) than cytokine regulation. BIT Life Sciences 2nd Annual World Vaccine Congress -2010 Beijing International Convention Center (BICC) Beijing China March 26 2010. 2. Working Group 14b. The Genomic Revolution: Implications for treatment and control of infectious disease. The 3rd National Academies Keck Futures Initiative, Irvine CA 2006. Platform Presentations:

1. Genetic Analysis of LPS responsiveness in The Gambian population. British Society for Histocompatibility and Immunogenetics. 10th Annual meeting Harrogate, UK., 10th February 1999.

2. Genetic regulation of immune response to TB. The joint meeting of International Union of Immunological Societies and Federation of Immunological Societies Yaounde, Cameroon. 14th -18th February 2000.

Poster Presentations:

1. Allelic mRNA expression imbalance in inflammasome related genes. AA Awomoyi, A Papp, G Smith, W Sadee M Wewers. The American Society of Human Genetics 59th Annual meeting Honolulu Hawai’i Oct 20th 24th 2009 2. Allelic mRNA expression imbalance in inflammasome related genes. AA Awomoyi, A Papp, G Smith, W Sadee M Wewers. Keystone Symposia Banff CA March 2009. 3. Differential Activation of Human TLR4 by Eschericia coli and Shigella flexneri 2a Lipopolysaccharide: Effect of TLR4 Polymorphisms on NF-K Activation and Cytokine Production. Joint SLB/IEIIS meeting San Antonio, Texas Nov 9-11 2006. 4. The 5’ Promoter region of the Human Solute Carrier family 11 member 1 protein

(SLC11A1): linking Infections, Autoimmunity and Cancer? 11th International Congress of Human Genetics, Brisbane Australia. 6th – 10th Aug 2006. 5. The 5’ Promoter region of the Human Solute Carrier family 11 member 1 protein

(SLC11A1): linking Infections, Autoimmunity and Cancer? The National Academies 3rd Futures Initiative. The Genomic Revolution: Implications for Treatment and Control of Infectious Disease. The Beckman Center 5251 California Ave, Irvine, CA., 92617,USA. 10th -13th Nov 2005.

6. Association of Toll-like receptor 4 (TLR4) Extracellular Polymorphisms with Severe Respiratory Syncytial Virus (RSV) Infection in Infants and Young Children. 46th Annual Short Course on Medical and Experimental Mammalian Genetics Bar Habor, Maine. USA. 17th-29th July 2005.

7. Global distribution if a novel trinucleotide microsatellite polymorphism (ATA) n in intron 8 of SLC11A1 gene and susceptibility to pulmonary tuberculosis. 53rd Annual American Society of Human Genetics Meeting Los Angeles CA 4th - 8th Nov 2003. 8. Microsatellite haplotype variability at the SLC11A1 locus in globally diverse human populations 6th International Meeting on Molecular Epidemiology and Evolutionary Genetics in infectious diseases MEEGID VI Institute Pasteur Paris, France. July 23rd - 27th 2002.

9. Global distribution if a novel trinucleotide microsatellite polymorphism (ATA) n in intron 8 of SLC11A1 gene and susceptibility to pulmonary tuberculosis. 53rd Annual American Society of Human Genetics Meeting Los Angeles CA 4th - 8th Nov 2003. 10. Microsatellite haplotype variability at the SLC11A1 locus in globally diverse human populations 52nd Annual American Society of Human Genetics Meeting Baltimore, MD., 15th-19th Oct. 2002.

11. Microsatellite haplotype variability at the SLC11A1 locus in globally diverse human populations 6th International Meeting on Molecular Epidemiology and Evolutionary Genetics in infectious diseases MEEGID VI Institute Pasteur Paris, France. July 23rd - 27th 2002.

12. Identifying tuberculosis disease resistance genes using Quantitative Phenotype Analysis. 51st Annual American Society of Human Genetics Meeting San Diego CA 12th -16th Oct. 2001.

13. Genetic regulation of immune response to TB. Society of leukocyte biology meeting Cambridge, UK., Sept 1999.

14. Host determinants of susceptibility to tuberculosis. Keystone Symposia. Colorado, USA., March 1998.

15. Host determinants of susceptibility to tuberculosis. Medicine and Research in the Tropics. Medical Research Council The Gambia. Jubilee meeting Nov 15th 1997. 16. Absence of TAP2D in Yoruba Nigerians. British Society for Histocompatibility and Immunogenetics Annual meeting Belfast January 5 th-7th 1994.

● Grant Writing Workshops attended:-

1. Grand Rounds:- Writing a competitive Grant Application by Wendy Sanders. November 17 2006.

2. Tips for applying for an NIH “K” Award by Wendy Sanders, Douglas D Ross and Miriam Smyth. May 7th 2007.

3. Writing a first grant application:- Biomedical communications workshop for postdoctoral fellows and junior faculty by Wendy Sanders and others. June 26th 2007 4. Giving a research talk and communicating to get the job:- Biomedical communications workshop for postdoctoral fellow and junior faculty by Wendy Sanders and others June 28th 2007 .

5. Grantsmanship Training Program FASEB Grant writing Seminars and Practical Exercises workshop. Getting started as a successful grant writer and Academician FASEB Campus Bethesda MD Jun 19 2009.

● Research Support:- United States of America:

1. Burroughs Wellcome Fund Career Award, a David and Lucile Packard Career Award and National Science Foundation grant to Dr Sarah Tishkoff. University of Maryland College Park MD 20742

2. National Institute of Health Immunology Research Unit grant no N01 AI30028 to Drs Marcelo Sztein and Stefanie Vogel. University of Maryland School of Medicine Baltimore MD 21201

National Institute of Health Ruth L Kirschstein National Research Service Award

(NRSA) Institutional Research Training Grant T32 To Professor Mark D. Wewers. Ohio State University Dorothy Davis Heart and Lung Research Institute Ohio State Medical Center.

Extra-curricular Activities: - Customer Service Experiences Walmart Port Covington, Maryland 2005-2006 :- Door Greeter Ross Dress for less Rockville-Pike, Maryland 2013 :- Overnight / Day Sales Associate Target Rockville –Pike, Maryland 2014- 2016 Softlines Fitting Room Sales Associate Target Sterling West, Virginia 2016 – 2018 Backroom Flexible Fulfilment Harris Teeter Lansdowne, Virginia 2016 - 2018 :- Overnight Cashier Home Depot Chantilly, Virginia 2018 ProDesk Associate Salamander Resort and Spa Middleburg, Virginia 2019 Spa Facilitator Maryland Insurance Producer License #2131867 (2014 – 2016). Currently studying towards obtaining a Diploma in Customer Services at ALISON online Education Services

Contact this candidate