Susan Egbert
ORCID: https://orcid.org/****-****-*458-1099
ad5tt7@r.postjobfree.com
Objective
I’m a postdoctoral fellow in Dr. Kian-Huat Lim’s lab research on therapeutic targets for pancreatic cancer. My interest in research started before pharmacy school and continued on during pharmacy school. Through researching in the lab, I have been able to learn various techniques. I am planning to pursue academia as I feel that there are many students that could become scientists but do not have the opportunity to do so. Education
Doctorate of Philosophy-Chemistry : 09/2019-12/2023 University of Manitoba, Winnipeg, MB
Various techniques include NMR, IR, mass spectrometry, culturing, and structure elucidation. Doctorate of Pharmacy : 08/2012- 05/2019
University of Louisiana at Monroe, Monroe, LA
Various training courses including:
Immunization certification through APhA, certification for use of vivarium, NMR course (graduate course audit)
Work Experience
Postdoctoral Fellow: 10/2023-present
Washington University at St. Louis, MO
I am working on trying to get a TPL2 KO cell line to inject into the mice. Additionally, I have conducted genotyping and breeding of mice to obtain a KPPC-TPL2-KO mice to investigate the mechanism of TPL2. During this time I have done other molecular biology techniques in addition to this. Research Assistant: 09/2019-09/23
University of Manitoba-Winnipeg, MB
I am responsible for learning various techniques to do structure elucidation (NMR, IR, mass spectrometry). Also, I’m working on the usnic acid biosynthesis pathway in lichens and trying to have functional expression of the lichen genes outside of lichens.
Teaching Assistant: 09/2019-05/2022
University of Manitoba-Winnipeg, MB
I am responsible for teaching students in the organic chemistry lab (CHEM 2210, 2220, 2122). I assist students in their experiments and help them understand why various techniques are necessary to learn. Pharmacy Intern: 07/2018 – 05/2019
Walgreens- Monroe, West Monroe, LA
I am responsible for inputting prescriptions, dispensing medications to patients, counsel patients on medications, provide MTM services, transfer prescriptions between pharmacies, and give immunizations to patients. I’ve also helped patients who were in buyout pharmacies transition into the Walgreens system. Pharmacy Intern: 08/2016-07/2018
Rite Aid-Monroe, West Monroe, Ruston, LA
I was responsible for inputting prescriptions, dispensing medications to patients, counsel patients on medications, provide MTM services, transfer prescriptions between pharmacies. I’ve also aided in closing of Rite Aid pharmacies and with transitioning Rite Aid pharmacies to Walgreens during the buyout. Pharmacy Intern: 07/2016-03/2017
Walmart, Rayville, LA
I was responsible for inputting prescriptions, dispensing medications to patients, counsel patients on medications, and manage inventory.
Research Experience
Washington University at St. Louis, MO (10/2023-present) 1. To understand the TPL2 mechanism in PDAC
a. KO using CRIPSR-CAS9
b. Breeding mice to achieve KPPC-TPL2
c. If a drug (tilpisertib) in the clinic is worth pursuing as a therapy option for PDAC. 2. Coding
a. Single cell analysis in R
b. Some basics in Python
University of Manitoba-Winnipeg, MB (09/2019-09/2023) 3. Evaluating different lichens in the herbarium on campus. Made crude extracts of lichens and isolated lichen secondary metabolites from extraction from lichens to using column chromatography to isolate metabolites. Metabolites were identified through TLC plates, NMR spectroscopy, and mass spectroscopy.
4. Evaluating protein structure of polyketide synthase domains and understanding the difference between different species
5. Managing collaboration projects with groups in both New York and Newfoundland. ULM-College of Pharmacy- Monroe, LA (03/2014-07/2019) 6. Collection of lichens from the field (Russell Sage Wildlife Refugee) and identification of lichens 7. Made crude extracts of lichens and isolated lichen secondary metabolites from extraction from lichens to using column chromatography to isolate metabolites. Metabolites were identified through TLC plates, NMR spectroscopy, and mass spectroscopy. 8. Biological assays were conducted to test metabolites through MTT assay, wound healing assay, invasion assay, and western blot assay.
9. Current project is isolation of fungi from lichens and seeing if they could keep the integrity and produce the same metabolites
Skills
o Software Skills: Word, Excel, Powerpoint, Schrodinger molecular modelling, Coding (R, Python) o Molecular Biology: Cloning, DNA isolation and purification, RNA isolation and purification, protein isolation and purification
o Analytical Technique: NMR, Thin Layer Chromatography, High-Performance Liquid Chromatography
(HPLC), Liquid Chromatography Mass Spectrometry (LC-MS) o In Vitro Studies: MTT, wound healing, invasion assays o Identification technique: identification of lichen and fungi through an identification key o Extraction of natural products through varying techniques Publication
Jiang, Y., Rex, D.A.B., Schuster, D., Neely, B.A., Rosano, G.L., Volkmar, N., Momenzadeh, A., Peters-Clarke, T.M., Egbert, S.B., Kreimer, S. and Doud, E.H., 2023. Comprehensive Overview of Bottom-up Proteomics using Mass Spectrometry. arXiv preprint arXiv:2311.07791. Covington, E.W., Jasper Trotter, S.L., Arnold, R.D., Amin, R., Egbert, S. and Chung, A., 2023. Prospective pilot study evaluating a vitamin D3 loading dose in critically ill children with vitamin D deficiency. Fundamental & Clinical Pharmacology.
Emily G. McDonald, MD MSc, Gloria Aggrey, MD, Abdullah Tarık Aslan, MD, Michael Casias, PharmD, BCIDP, AAHIVP, Nicolas Cortes-Penfield, MD, Mei Qin (Denise) Dong, Pharm.D., Susan Egbert, PharmD, Brent Footer, Pharm.D., et al. Guidelines for Diagnosis and Management of Infective Endocarditis in Adults: A WikiGuidelines Group Consensus Statement. JAMA Netw Open. 2023;6(7):e2326366. doi:10.1001/jamanetworkopen.2023.26366 Li, X., Ndungu, P., Taneja, S.B., Chapin, M.R., Egbert, S.B., Akenapalli, K., Paine, M.F., Kane Gill, S.L. and Boyce, R.D., 2023. An evaluation of adverse drug reactions and outcomes attributed to kratom in the US Food and Drug Administration Adverse Event Reporting System (FAERS) from January 2004 through September 2021. Clinical and Translational Science. Egbert, S., Hoffman, J. R., McMullin, R. T., Lendemer, J. C., & Sorensen, J. L. (2022). Unraveling usnic acid: A comparison of biosynthetic gene clusters between two reindeer lichen (Cladonia rangiferina and C. uncialis). Fungal Biology.
Terlouw, B. R., Blin, K., Navarro-Muñoz, J. C., Avalon, N. E., Chevrette, M. G., Egbert, S., ... & Medema, M. H. (2022). MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters. Nucleic Acids Research.
Patient and Herbal Supplement
February 2018-Drug Topics article
https://www.drugtopics.com/view/patients-and-herbal-supplements Ebrahim HY, Elsayed HE, Mohyeldin MM, Akl MR, Bhattacharjee J, Egbert S, El Sayed KA. Norstictic Acid Inhibits Breast Cancer Cell Proliferation, Migration, Invasion, and In Vivo Invasive Growth Through Targeting C-Met. Phytother Res. 2016 Apr;30(4):557-66. doi: 10.1002/ptr.5551. Epub 2016 Jan 6. PubMed PMID: 26744260; PubMed Central PMCID: PMC5045260.
o Extraction of natural products through varying techniques Presentation
Investigation of the undiscovered potential for lichen-fungi metabolites (poster) July 2023 ASP
Investigation of the undiscovered potential for lichen-fungi metabolites (poster) May 2023 Ft. Detrick Spring Research Festival
Outstanding Poster Award in Applied and Environmental Biology Lichens, an underexplored symbiotic organism, issues in literature, and future potentials. (oral) March 2023 CanFunNet
Investigation of the undiscovered potential for lichen-fungi metabolites (poster) February 2023 NIH Graduate Student Symposium
A New Perspective On Usnic Acid Biosynthesis In Cladonia Rangiferina (oral) Sept 5, 2022 ICPS
Identifying the Squamatic Acid Biosynthetic Gene Cluster in Lichen Cladonia uncialis (poster) July 2022 ASP
Identifying the Squamatic Acid Biosynthetic Gene Cluster in Lichen Cladonia uncialis (oral) June 3, 2022 CanFunNet
A New Perspective on Usnic Acid Biosynthesis in Cladonia rangiferina (poster) October 18-21, 2021 MANA 2021
A New Perspective on Usnic Acid Biosynthesis in Cladonia rangiferina (poster) July 16, 2021 ASP
What do we really understand about lichen biosynthetic gene cluster (oral) May 26, 2021 MSA
https://www.youtube.com/watch?v=yHUCPw1q-jc
Lichen-Past, Present Future (oral)
May 4, 2021 Torrey Botanical Society
Judging
Undergraduate medical student research program
August 12, 2021 University of Manitoba-Faculty of Medicine 3MT competition (undergraduate)
August 10, 2021 University of Manitoba-Faculty of Science Extracurricular Activities/Organizations
LOGIC (WIC) retreat 2022
TBA
Position held: co-chair
CCCE 2021
August 15-21, 2021
Position held: volunteer
LOGIC (WIC) retreat 2021
August 7-13, 2021
Position held: organizer
PhD Balance
June 2021-present
Stronger Together Team leader (August 2021-Dec 2021) Vice President (October 2021-Dec 2021)
President (Jan 2022- )
American Pharmacist Association-Academy of Pharmaceutical Research and Science (APhA-APRS) June 2021-present
Committee member
University of Manitoba International Student Association (UMISO) May 2021-present
Vice President Logistics
Females in Mass Spectrometry (FeMS) Mentoring Program April 2021-present
Planning committee
Summer project supervisor
May 2020-August 2020
Supervised an undergraduate student researching the synthesis of lichen secondary metabolites. U of M Womyn’s Centre
July 2020-May 2021
Vice President Social Media
LOGIC (WIC) retreat 2020
May 17-23, 2020
Position held: organizer
COVID-19 translation
April 2020-May 2020
Science Fair Project coordinator
Jan 2020-March 2020
Supervised elementary students with their science fair project. This involved looking into the bioactive compounds of cinnamon and testing its activity against pathogenic bacteria. CWIC (Canadian Working Towards Inclusivity in Chemistry) Jan 2020-present
Incoming CFO (May 2021-present)
Women in Chemistry/Working Towards Inclusivity in Chemistry (WIC) January 2020-Present
Co-President (Internal May 2021-present)
Co-Vice president Recruitment (July 2020- May 2021) U of M Chemistry Graduate Association
September 2019-Present
ULM Student Research Club
January 2018-May 2019
Positions held: Vice President, President
ULM Global Ambassador
May 2016-May 2019
Positions held: Vice President, President, Grad Liaison Events Held: Health Fair, Art Crawl, Orientation for International Students ULM Student Research Symposium
March 2016, March 2017, April 2018
ULM APhA-ASP
August 2015-May 2019
ULM American Chemistry Society
January 2014-May 2015
Position held: Historian
ULM Pre-Pharmacy Organization
January 2013-May 2015
ULM Honors Program
August 2012-May 2019
Positions held: Freshmen Representative, Treasurer, President, Grad Liaison. Thesis defense: May 2015
Awards: Student of the Year (two years in a row), Freshmen of the Year Training
2021 VADA Program Data Science and AI Summer School July 13-15, 2021
Reviewing
2021 CADTH Symposium
July 2021
Abstract Reviewer
UMGSA awards
Summer 2021