Chemical Engineering Research Associate

Education:

Northeastern University Boston, MA Master’s Degree in Chemical Engineering: GPA 3.586 Sept 2018-Dec 2020

Rutgers The State University of New Jersey New Brunswick NJ Bachelor’s Degree in Chemical Engineering: GPA 3.41 Sept 2012-Oct 2016

Work Experience

Senior Research Associate– Decibel Therapeutics Jan 2023 – Feb 2024

• (LNP) Lipid nanoparticle particle transfection, AAV transduction, RNAdvance isolation with RT-qPCR analysis

•HEK293T, HEK293A, Hela cell culture, SAM D73 crispr cell line maintenance, automated fluorescence counting with Sony Cell Sorter, Guava Flow Cytometry for high throughput potency assay, ELISA for potency assays, Enzymatic Assays, Gel Electrophoresis for Western Blotting, tissue culture

•Benchling plasmid check in and design, plasmid dilution, promotor expression, dirty AAV preps from cell lysates and supernatants, Recording experimental protocols and data on electronic lab notebook (ELN)

•Method development for functional assay for hearing disorder programs of pendrin, stereocilin, and otoferlin

Scientist I – Axcella Therapeutics Nov 2022 – Dec 2022

•Human Lung Microvascular Endothelial Cell culture, CTF reading, RNA Isolation, DAPI cell stain counting

•ELISA based testing for markers for NASH and long covid, PCR, DNA preparation for molecular biology

•Tryzol lysis with zymo kit based RNA isolation, electronic multichannel micropipette

Scientist I – Thermofisher Scientific Jan 2022 – Oct 2022 •qPCR, QuantStudio Flex 7, cell free DNA isolation, Tumor Cell Culture, IonTorrent sequencing

•King Fischer automated DNA isolation, E.coli transformation, Midi-Prep Kit Isolation, Nuclei Acid Synthesis

Scientist I - Sanofi Mar 2021 – Dec 2021 •Immunological Assays such as MSD, ELISA, ELISpot, flow cytometry and RT-qPCR

•Flow cytometry with antibody fluorophores for cell sorting and quantification (FITC, BUV395, PE, PE/Cy7, etc)

•Radiation pulsing with tritium thymine to measure cell proliferation, In vitro Immunofluorescence

•T cell isolation, transfection, and culturing, CD3 stimulation, CHO TCRa stimulation, moDC isolation, differentiation, and T cell coculture, tissue culture, immunochemistry

•Splenocyte mice extraction and culturing, Cardiac blood mice extraction and culturing. Mouse CO2 euthanasia

•Small molecule dosing assay with IgG and IgM antibodies for immune checkpoint markers, LNP encapsulated RNA transfection of immune cells

Research Student (Retinal Regeneration) - Northeastern University Nov 2018 – Mar 2021 •Morphology DAPI and Alexfluoro staining to qualify cell differentiation within different hydrogels conjugated to small molecules, quantitative image analysis

•CCK spectroscopy, human retinal progenitor cell culturing and IPSC differentiation.

•Hydrogel conjugation to small molecules like RGD and YSIR via oxidation

Skills

Programs: MATLAB, Simulink, Maple, SolidWorks 2017, LIMS software, Benchling, PRISM, FlowJo, ImageJ

Laboratory Procedures: Biological Safety Level 2, GMP stem cell culturing, plasmid transfection, Cytometry, ELISA, Tritium Pulsing

Instrumentation: Fortessa Flow Cytometer, Sony cell sorter, QuantStudio PCR, Electronic Micropipetes, KingFischer DNA isolator, Incucyte,

References

Rebecca Carrier, Vijay Patel, Hemax Patel, Shishir Chundawat

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