Maria Naim Rasheed

**** ******* **** **, ********, Texas 77407 · 832-***-**** · ad5axa@r.postjobfree.com EDUCATION

BS, Neuroscience: College of Arts and Sciences

Georgia State University, Atlanta, Georgia

MS, Neuroscience: Graduate School of Biomedical Sciences University of Texas Health and Science Center, Houston, TX EXPERIENCE

GRADUATE RESEARCH ASSISTANT, JOHN BYRNE - UT HEALTH, HOUSTON, TX Aug.2022 - Present

• Conducted in-depth research on the effects of operant conditioning on neuronal excitability.

• Utilized electrophysiological techniques, including patch-clamp recordings, to measure membrane potentials and action potentials.

• Employed MATLAB for data analysis, extracting key biophysical properties and conducting statistical analyses.

• Developed conductance-based models using MATLAB to simulate the biophysical properties of motor neurons.

• Programmed scripts to read and process data files, ensuring accuracy in empirical model simulations.

• Collaborated with a multidisciplinary team to integrate findings into a comprehensive computational model of the feeding central pattern generator.

• Thesis work contributed to understanding the dynamics of neurons and their role in behavior modification.

RESEARCH ASSISTANT II, DONG KIM LAB - MD ANDERSON, HOUSTON, TX Mar. 2019 – Aug.2022

• Identifying how rare THSD1 variants and loss of function impact the brain in IA and SAH cases

• Conducting various experiments on genetically engineered mice

• DNA & RNA isolation of rodent tissue Specifically Circle of Willis

• DNA & RNA isolation of Human samples of blood, saliva and pineal cysts

• Preforming various perfusions methods including saline, India ink and gelatin, Evan and Bromophenol blue dye, latex, corrosion vascular casting

• Immunohistochemistry to determine cell proliferation, apoptosis, and other possible cell abnormalities

• Disassociation with dissected Circle of Willis for RNA-Seq

• Organizing and maintaining mouse colony

• Genotyping and breeding

• Applying hypertension models using both diet and surgical methods

• Monitor blood pressure on mice using a tail cuffing method RESEARCH ASSISTANT I, DO MONTE LAB – UT HEALTH, HOUSTON, TX Aug. 2017- Mar. 2019

• Behavioral experiments reward, fear, and stress in rats

• Histological analysis of brain tissue

• Collecting, staining, and analyzing vaginal cells to determine the estrous cycle phase in female rats

• Constructing optical fibers and patch cords for optogenetic experiments

• Techniques including optogenetics, chemogenetics, and viral tracer studies

• Performing immunohistochemistry for identification of specific brain proteins

• Hand-scoring and analyzing behavioral data using Any-Maze and Excel software

• Working independently in a project to identify sex differences in stress and foraging behavior

• Taking care of the rat colony inventory, handling, and training animals to bar- press for food and performing administrative duties in the lab.

• Presenting scientific articles at Journal Clubs. BEHAVIORAL RESEARCH INTERN, GEORGIA AQUARIUM, ATLANTA, GA Jan. 2016 - Dec. 2016

• Behavior research training

• Observing, monitoring, and recording data of whale sharks

• Record any unusual activity according to eating behavior and swimming patterns then comparing them to collected baseline data for whale sharks GSU RESEARCH STUDENT, NEUROSCIENCE LAB, ATLANTA, GA Jan. 2016 - Mar. 2016

• Experimenting on crustaceans

• Applying Nicotine to ACh-nicotinic receptors and recording activity

• Using auditory stimulus to determine Stress-responsive behavior

• Performed Immunocytochemistry on nerve cords

• Surgeries and planted electrodes to record using electrophysiology

• Using PCR then examining the results using gel electrophoresis CLINICAL RESEARCH ASSISTANT, PROOVE BIOSCIENCES, ATLANTA, GA May. 2015 - Dec. 2015

• Working with Doctor of Osteopathic Medicine

• Screening patients and determining whether they qualify for opioid risk testing

• Collect saliva samples from patients to send out for sequencing

• Review sequencing results with patients to assess their tolerance to pain and appropriate drug intake

• Reviewing results with doctors to assist with prevention of opioid addiction RESEARCH ASSISTANT, GEORGIA STATE UNIVERSITY, ATLANTA, GA May. 2014 - Apr. 2015

• Learning about socialization and motivation of same-sex and opposite sex in male hamsters.

• Assisting with behavioral tests and recording behaviors

• Learning and training about Polysialyted Neural Cell Adhesion molecule

• Staining using immunohistochemistry to visualize the PSA-NCAM.

• Helping with injection of the Endo-alpha-sialidas (Endo-N) in the MePD to prevent emergence of preference.

• Collecting and organizing experimental data

VOLUNTEER EXPERIENCE

MEDICAL ASSISTANT, SHIFA CLINIC, DULUTH, GA Jan. 2014 - Jan. 2017 Maria Naim Rasheed

9327 Mineral Rock Ln, Richmond, Texas 77407 · 832-***-**** · ad5axa@r.postjobfree.com

• Front Desk Administrator: Patient registration, medical record keeping, ultrasound scheduling, resource referrals (mammogram), administrative work, delegate patients to doctors, and creating new handbooks.

• Lab Administrators: Fill out lab forms, calculate pricing of the lab work done, collecting payments, and logging the patients’ test results

• Medical Assistant: checking and recording blood pressure, weight, temperature, patient history, and EKG

• EMR System Manager: Transferring paper charts to EMR and leading a team

• Equipment Manger: Order supplies, contact local hospital for donation of equipment and services for the clinic.

• Health Fair Coordinator: Appoint volunteers in the monthly health fairs, host meetings on expectations of the specific health fair, and create events.

• Health Fair Volunteer: Testing patients’ lipid panel, glucose, blood pressure, body mass index.

WISH GRANTER, MAKE-A-WISH, ATLANTA, GA May 2014 - Jan. 2017

• Being an advocate for each child with a terminal illness

• Coordinating and organizing details per specific wish

• Bringing motivation and encouragement to the families PRESENTER, DISCOVERY DAY - ATLANTA SCIENCE FESTIVAL, ATLANTA, GA Jan. 2015 - May 2015

• Presenting the anatomy of the brain regions and basic functions

• Demonstrating fun and interactive ways to engage and teach audience about neuroscience.

• Giving visitors of all ages the opportunity to get involved and gain their curiosity and possible exploration into science.

ACQUIRED SKILLS

Program scripts in RStudio and MATLAB for data analysis, statistical analysis, computational modeling, ensuring accuracy in empirical model simulations and leveraging artificial intelligence techniques for enhanced data analysis. Rodent tail cuff blood pressure, latex casting, microfil perfusion, carotid artery ligation, Retro-Orbital Injection, Intracranial injections, fear and reward conditioning, vaginal lavage for estrous cycle staining, transcranial perfusion, histology, electrophysiology, immunohistochemistry, PCR, ELISA, DNA/RNA extraction of human blood, saliva, and pineal cysts. POSTERS IN SCIENTIFIC CONFERENCES

Engelke, DS; Terzian, AL; Rasheed, MN; Fernandez-León, J; Do Monte, FH. Paraventricular thalamus balances reward seeking with the risk of predation. Federation of European Neuroscience Societies meeting 2018, Berlin, Germany. Fabricio H. Do Monte, DVM, PhD Page Fernandez-León, J; Engelke, DS; Rasheed, MN; Terzian, AL, Do Monte, FH. Neuronal correlates of reward vs. fear memory discrimination in the prelimbic cortex. Society for Neuroscience meeting 2018, San Diego, CA.

Terzian, AL; Engelke, DS; Rasheed, MN; Li, S; O’Malley, JJ; Dasgupta, R; FernandezLeón, J; Justice, NJ; Beierlein, M; Kirouac, GJ; Do Monte, FH. CRF neurons in the paraventricular thalamus reduce food- seeking behavior. Society for Neuroscience meeting 2018, San Diego, CA. Engelke, DS; Fernandez-León, J; Terzian, A.L.; Rasheed, MN; Do Monte, FH. Balancing food seeking with the risk of predation recruits the paraventicular thalamus. Society for Neuroscience meeting 2018, San Diego, CA.

Engelke, DS; Rasheed, MN; Terzian, AL; Fernandez-León, J; Olivo, L; Do Monte, FH. Elucidating the neural circuits regulating emotional memories. GSBS Poster Session 2018, Houston, TX. Zhang, X; Terzian, AL; Engelke, DS; Rasheed, MN; Li, S; O’Malley, JJ; Dasgupta, R; Fernandez-León, J; Justice, NJ; Beierlein, M; Kirouac, GJ; Do Monte, FH. CRF neurons in the paraventricular thalamus reduce food-seeking behavior. UTH Poster Session 2018, Houston, TX. PUBLICATIONS:

Fernandez-Leon, J. A., Engelke, D. S., Aquino-Miranda, G., Goodson, A., Rasheed, M. N., & Do-Monte, F. H. (2021). Neural correlates and determinants of approach-avoidance conflict in the prelimbic prefrontal cortex. https://doi.org/10.1101/2021.05.27.445881

Yan, Y., Martinez, R., Rasheed, M. N., Cahal, J., Xu, Z., Rui, Y., Qualmann, K. J., Hagan, J. P., & Kim, D. H.

(2021). Germline and somatic mutations in the pathology of pineal cyst: A whole exome sequencing study of 93 individuals. Molecular Genetics & Genomic Medicine, 9(6). https://doi.org/10.1002/mgg3.1691

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