Dr. Subramanyam Kondeti

National Institute of Agricultural Botany (NIAB), Cambridge–CB24 9NZ, England, United Kingdom.

+44-745*******; ad4qjb@r.postjobfree.com; Dr. Subramanyam Kondeti SUMMMARY

Enthusiastic researcher with an immense inquisitive mind and an avid interest in the field of Plant Biotechnology. My research expertise relies on various regeneration and genetic transformation techniques to generate transgenic crops towards improved nutritional quality and stress resistance. Backed by a solid educational background, experience gathered by working in a well reputed research laboratories and projects from different Biotechnology funding agencies has given me a deep understanding of plant genetic modification towards the crop improvement. EXPERIENCE

Scientist – Plant Transformation; August 2021 – present Department of Crop Transformation, NIAB, Cambridge, United Kingdom. Advisor: Dr. Emma Wallington I am involved in using various regeneration and genetic transformation technologies to establish the genetic transformation platforms for the economically and commercially important legume species including soybean, faba bean, common bean, cowpea, and Medicago.

Postdoctoral Research Associate; January 2019 – August 2021 Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA. Advisor: Prof. Neal Stewart Jr Under the supervision of Prof. Neal Stewart Jr, a respected authority in the field of plant synthetic biology I have contributed to establishing the high throughput soybean protoplast transfection and attracted a research funding from BASF to elucidate the phytotoxic severity of certain groups of genes and development of hppd inhibitor resistant callus from soybean protoplasts. During my tenure at this laboratory, I was also involved in one of the DARPA- APT project objectives dealing with the Agrobacterium-mediated genetic transformation of phytosensor constructs into a wide range of plant species.

Postdoctoral Research Assistant; January 2015 – July 2018 Department of Molecular Biotechnology, Ghent University, Ghent, Belgium. Advisor: Prof. Els JM Van Damme During my tenure I had the privilege of working alongside Prof. Els Van Damme, a well-known glycobiology Professor. Under her supervision, I have performed research related to several abiotic and biotic stress assays and studied the Euonymus related lectins (EUL lectins) expression in rice. Established rice transformation platform and developed abiotic and biotic stress resistant transgenic rice plants harboring the EUL genes. Graduate Research Fellow (Ph.D research scholar); July 2009 – December 2014 Department of Biotechnology, Bharathidasan University, Tamil Nadu, India. Advisor: Prof. Andy Ganapathi During my PhD, I was actively involved in several research projects focusing on improving nutritional quality and abiotic stress resistance in several crop plants. One such study which led to my doctoral dissertation work involved the study of establishment of somatic embryogenesis and Agrobacterium-mediated genetic transformation protocols for India soybean cultivars and developed transgenic soybean harboring tobacco osmotin

(Tbosm) gene confirming resistant to salinity and fungal diseases. Senior Research Fellow; Jan 2009 – April 2014

Department of Biotechnology, Bharathidasan University, Tamil Nadu, India. Advisor: Prof. Andy Ganapathi During my tenure, I was involved in a group developing Agrobacterium-mediated genetic transformation protocols for Podophyllum hexandrum to improve podophyllotoxin content. Performed Agrobacterium-mediated genetic transformation of several commercial and vegetable crops including, sugarcane, Jatropha curcas, eggplant, okra, and snake gourd.

Research Assistant, December 2003 – December 2008

Shreedhar Bhat Laboratory, Bangalore, Karnataka, India I was assisting the genetic engineers in generating the transgenic capsicum and banana plantlets. Individually, I established a highly efficient genetic transformation method for banana cv. ‘Rasthali’ by employing the various wounding methods during the transformation. In addition, I was also working on genetic purity analysis of hybrid seeds and involved in commercial scale Taq DNA polymerase purification. EDUCATION

Doctor of Philosophy (Ph.D.,), Biotechnology

Bharathidasan University • Tamil Nadu, India • 2014. Master of Science (M.Sc.,), Biochemistry

Sri Krishnadevaraya University • Andhra Pradesh, India • 2003 COURSEWORK

Basic molecular biology techniques

Sri Krishnadevaraya University, Andhra Pradesh, India • 2004 Plant tissue culture and genetic transformation.

Shreedhar Bhat Laboratory, Bangalore, India • 2003 Recombinant DNA technology.

BEST biotech research labs, Bangalore, India • 2003 AWARDS

BOF Postdoctoral Research Assistantship from Ghent University, Belgium • 2015 Senior Research Fellowship (SRF) award from CSIR, India • 2012 Senior Research Fellowship (SRF) award from DRDO, India • 2009 TECHNICAL SKILLS

Strategic design: planning, and execution of research projects. Analysis of results, writing manuscripts, answering reviewers’ comments, and reviewing the manuscripts received from peer reviewed plant biology journals. Plant tissue culture and genetic transformation: Different methods of plant regeneration (direct and indirect organogenesis, and somatic embryogenesis), initiation and maintenance of cell suspension cultures, Agrobacterium- mediated genetic transformation of several crop species, particle bombardment, In-planta transformation, Arabidopsis floral dip transformation, protoplast transfection. Molecular biology: Plasmid DNA, genomic DNA, and RNA isolation, preparation of over expression and CRISPR- CAS9 constructs using traditional cloning and gateway cloning, screening of transgenic plants by polymerase chain reaction, Southern blot hybridization, gene expression studies using qRT-PCR, DNA finger printing techniques such as RAPD and SSR.

Plant stress physiology: Different abiotic (salinity drought, high temperature, cold, heavy metal, and hormones) and biotic stress (bacteria, fungus, and insects) experiments, antioxidant enzyme activity assays. Protein biochemistry: Expression of cloned genes in E. coli and Pichia pastoris, protein isolation and purification, SDS- PAGE, Western blotting, immunoprecipitation.

RESEARCH PUBLICATIONS

Subramanyam K, Sailaja KV, Muralidhara Rao D, Lakshmidevi K (2011) Highly efficient Agrobacterium-mediated transformation of banana cv. Rasthali (AAB) via sonication and vacuum infiltration. Plant Cell Rep 30:425–436.

Subramanyam K, Sailaja KV, Subramanyam K, Muralidhara Rao D, Lakshmidevi K (2011) Ectopic expression of an osmotin gene leads to enhanced salt tolerance in transgenic chilli pepper (Capsicum annum L.). Plant Cell Tiss Organ Cult 105:181–192.

Subramanyam K, Arun M, Mariashibu TS, Theboral J, Rajesh M, Singh NK, Manickavasagam M, Ganapathi A (2012) Over-expression of tobacco osmotin (Tbosm) in soybean conferred resistance to salinity stress and fungal infections. Planta 236:1909–1925.

Subramanyam K, Rajesh M, Jaganath B, Vasuki A, Theboral J, Ilayaraja D, Karthick S, Manickavasagam M, Ganapathi A (2013) Assessment of factors influencing the Agrobacterium- mediated In-Planta seed transformation of brinjal (Solanum melongena L.). Appl Biochem Biotech 171:450–468.

Subramanyam K, Arunachalam C, Thaneswari RM, Sulaiman AA, Manickavasagam M, Ganapathi A (2015) Highly efficient Agrobacterium-mediated in planta genetic transformation of snake gourd (Tricosanthes cucumerina L.). Plant Cell Tiss Organ Cult 123: 133–142.

Subramanyam K, Du Laing G, Van Damme EJM (2019) Sodium selenate treatment using a combination of seed priming and foliar spray alleviates salinity stress in rice. Front Plant Sci 10:116

Mariashibu TS*, Subramanyam K*, Arun M, Theboral J, Rajesh M, Kasthuri Rengan, Chakravarthy R, Manickavasagam M, Ganapathi A (2013) Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars. Ind J Exp Biol 51:849–859. *Equally contributed

Mariashibu TS*, Subramanyam K*, Arun M, Mayavan S, Rajesh M, Theboral J, Manickavasagam M, Ganapathi A

(2013) Vacuum infiltration enhances the Agrobacterium- mediated genetic transformation in Indian soybean cultivars. Acta Physiol Plant 35:41–54. *Equally contributed

Mayavan S*, Subramanyam K*, Jaganath B, Sathish D, Manickavasagam M, Ganapathi A (2015) Agrobacterium- mediated in planta genetic transformation of sugarcane setts. Plant Cell Rep 34:1835–1848. *Equally contributed

Arun M, Subramanyam K, Theboral J, Sivanandhan G, Rajesh M, Kapil Dev G, Jaganath B, Manickavasagam M, Girija S, Ganapathi A (2013) Transfer and targeted overexpression of γ- tocopherol methyltransferase (γ-TMT) gene using seed specific promoter improves tocopherol composition in Indian soybean cultivars. Appl Biochem Biotech 172:1763–1776.

Jaganath B, Subramanyam K, Mayavan S, Karthik S, Elayaraja D, Udayakumar R, Manickavasagam M, Ganapathi A

(2013) An efficient in planta transformation of Jatropha curcas and multiplication of transformed plants through in vivo grafting. Protoplasma 251:591–601.

Mayavan S, Subramanyam K, Arun M, Rajesh M, Kapil Dev G, Sivanandhan G, Jaganath B, Manickavasagam M, Selvaraj N, Ganapathi A (2013) Agrobacterium tumefaciens-mediated In- Planta seed transformation strategy in sugarcane. Plant Cell Rep 32:1557 – 1574.

Arun M, Subramanyam K, Karthik S, Mariashibu TS, Theboral J, Manickavasagam M, Ganapathi A (2014) Application of sonication in combination with vacuum infiltration enhances the Agrobacterium-mediated genetic transformation in Indian soybean cultivars. Appl Biochem Biotech 175:2266–2287.

Arun M, Subramanyam Kondeti, Theboral J, Ganapathi A, Manickavasagam M (2014) Optimized shoot regeneration for Indian soybean: the influence of exogenous polyamines. Plant Cell tissue and Organ Culture 117:305–309.

Manickavasagam M, Subramanyam K, Ishwarya R, Elayaraja D, Ganapathi A (2015) Assessment of factors influencing the tissue culture-independent Agrobacterium-mediated in planta genetic transformation of okra

[Abelmoschus esculentus (L.) Moench]. Plant Cell Tiss Organ Cult 123:309–320.

Vasudevan V, Subramanyam K, Elayaraja D, Karthik S, Vasudevan A, Manickavasagam M (2017) Assessment of the efficacy of amino acids and polyamines on regeneration of watermelon (Citrullus lanatus Thunb.) and analysis of genetic fidelity of regenerated plants by SCoT and RAPD markers. Plant Cell Tiss Organ Cult 130:681– 687.

Elayaraja D, Subramanyam K, Vasudevan V, Satish S, Kasthurirengan S, Ganapathi A, Manickavasagam M (2019) Meta-topolin (mT) enhances the in vitro regeneration frequency of Sesamum indicum (L.). Bio Cat Agri Biotechnol 21:101320.

Rajesh M, Sivanandhan G, Subramanyam Kondeti, Kapil Dev G, Jaganath B, Kasthurirengan S, Manickavasagam M, Ganapathi A (2014) Establishment of somatic embryogenesis and podophyllotoxin production in liquid shake cultures of Podophyllum hexandrum Royle. Industrial Crops and Products 60:66–74.

Theboral J, Sivanandhan G, Subramanyam K, Arun M, Selvaraj N, Manickavasagam M, Ganapathi A (2014) Enhanced production of isoflavones by elicitation in hairy root cultures of soybean. Plant Cell tissue and Organ Culture 117:477–481.

Sivan Kalyani V, Geetha M, Subramanyam K, Girija S (2014) Ectopic expression of Arabidopsis RCI2A gene contributes to cold tolerance in tomato. Transgenic Res 24:237–251.

Arun M, Arunachalam C, Subramanyam K, Karthik S, Sivanandhan G, Theboral J, Sulaiman AA, Chang KK, Ganapathi A (2016) Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean. 3 Biotech 6:148.

Kumari A, Hada A, Subramanyam K, Theboral J, Misra S, Ganapathi A, Malathi VG (2018) RNAi-mediated resistance to yellow mosaic viruses in soybean targeting coat protein gene. Acta Physiol Plant 40:32.

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