Hong Sun

***** ***** **** *****, **********, MD 20871

Email: acxed2@r.postjobfree.com Phone: 240-***-**** (C)

SUMMARY

Scientist/ Associate Scientist with broad experience in histopatholgy, immunocyto/histochemistry (ICC/IHC) and microscopy and image analysis in support of R&D in the fields of neuroscience, autoimmune disease, oncology and drug discovery. Core strengths include histological technologies, immunostaining(ICC/IHC) and operating micropscopy. Proficiency in application of cellular biomarkers to develop IHC assay and image analysis for target evaluation and mechanisms of action. Expertise utilizing cell and tissue structures and staining patterns to identify sub-cellular and tissue recognition features to interpret results of quantitative and qualitative image analysis.

TECHNICAL SKILLS

Standard Histological Techniques: Necropsy; Tissue dissecting, perfusion fixation, processing and embedding; Microtomy including rotary microtome(paraffin), cryomicrotome (frozen), ultramicrotome (Plastic) and vibratome (fresh or fixed tissue) sectioning, semithin and ultrathin sectioning; HE and special staining.

Immunohisto/cytochemistry (IHC/ICC): Immunohistochemistry (ABC); Immunofluorescence staining including double or triple labeling; Familiar with application of cell biomarker, including immune cell and neuronal cells. Expertise in designing and performing IHC/ICC assays, including antibody validation, antigen retrieval processing and assay optimization.

Microscopy and Image Analysis: Light, fluorescence, confocal, and electron microscope. Ability to identify all tissues microscopically and review tissue section images through detailed observation, including histology and pathology of immune organ and tissue. Digital scanner microscope (Aperio) and quantitative image analysis.

Histology Lab Management Writing SOP and method; Archiving and labeling blocks, slides, and wet tissue on a routine basis; Purchasing supplier and reagent; Antibody log sheets updated; Maintenance instrument.

Cell Culture, Cell-based Assay and Molecular Biology Techniques: Multiple cell culture, Cell-based assay and immunoassay including ELISA, ECL and flow cytometry (FACS); PCR; Western Blotting.

Computer Software Microsoft Office; ImageJo, ImagePro-Analyzer; Aperio Spectrum System and Aperio eSlide Manager; Definiens; Photoshop, Graphpad;

PROFESSIONAL EXPERIENCE

Medimmune Inc.

Associate Scientist II 01/2014~08/2016

Conducted routine histology and pathology to evaluation of multiple autoimmune disease, inflammation and pulmonary fibrosis in vivo model and human samples.

Performed IHC, microscopy and image analysis to evaluate of multiple autoimmune disease and antibody efficacy in mouse models.

Developed multiple biomarker assays (ICC/IHC) to confirm being a target therapeutic cell in vivo and in vitro in immune cells of autoimmunity.

Explored a new method with fluorescence in situ hybridization (FISH) signals of telomere in the individual nuclei to analysis manifestation of tumor and aged tissue.

Managed and guided the method and SOPs of IHC/ICC in the lab, worked on scientific review, presentation and literatures to contribute at least 3 paper publications.

Covance Inc.

Research Associate, 11/2012~12/2013

Maintaining study documentation and laboratory records according to GLP regulation and performing quality control review of data.

Being responsible for conducting and evaluating the results of immunochemistry assay, and performing method development/validation and clinic trial samples assay in GLP lab.

MedImmune Inc.

Associate Scientist, 05/2011~11/2011

Validated new antibodies and optimized staining procedures and conditions for IHC in normal and autoimmunity disease animals.

Developed multiple immune cell biomarker assays and conducted immunohistochemistry, with parallel flow cytometry, to confirm the theoretic effects of monoclonal antibodies on immune cells in models of autoimmunity.

Conducted EAE animal clinic scoring, performed histopathology and IHC evaluation of demyelination and inflammation in spinal cord and brain to evaluate antibody efficacy in mouse models.

Otsuka Maryland Medicinal Laboratories, Inc. (OMML),

Senior Research Associate 02/2006~03/2011

Developed immunohisto/cytochemistry (IHC/ICC) special staining and neuronal biomarker application to provide evidence in support of stem cell being a target therapeutic cell in vivo and in vitro.

Designed, organized and coordinated execution of immunohistochemistry and image analysis to identify the fate and differentiation of human stem cells in different environments of host animal model, including spinal cord injury and immunodeficient disease.

Provided histopathology, immunoflurescence study and cell ultrastucture evidence to demonstrate the characteristics of degeneration disease in multiple animal models, including spinal cord injury, multiple sclerosis, cardiac ischemia, vascular vessel injury and toxicology in kidney and liver.

Conducted extensive immunocytochemistry (ICC) and neuronal cell based pharmacological assay to perform primary and log dose response testing of small molecule and /or biotherapeutics resulting in 25 possible lead compounds.

Confirmed mechanism of action studies in phospholation of synapsin of synaptic protein by performing ICC and image analysis to test possible lead compounds for drug target identification.

Wright State University Boonshoft School of Medicine,

Research Associate 02/2002~01/2006

Conducted extensive histology and immunocytochemistry (IHC) to explore hundreds of antibodies on neuron and synapses resulting in 5 antibodies applications for synaptic biomarker.

Explored, through immunohistochemistry and microscopy, to establish effective methodologies and image analysis that display their structural and functional characteristics during development.

Developed deaf animal models that disrupted normal tonotopic gradients in synaptic structure and led to establishment of deaf animal model for researching human congenital deafness.

EDUCATION

Master’s Degree Histology, Lanzhou Medical College (now part of Lanzhou University.) Lanzhou, China

Bachelor's Degree, Medicine, Lanzhou Medical College (now part of Lanzhou University) Lanzhou, China

ADDENDUM

Selected Publications and Abstracts

Ana Camelo, Timothy Burwell, Alan Carruthers, David M. Habiel, Jenny Kearley, Aaron Berlin, Hong Sun, Jane Connor, Ana Lucia Coelho, Lori Clarke, Ethan Grant, Arthur Lewis, Elena Miranda, Bethany Moore, Tomas Mustelin, Matthew A. Sleeman, Erica L. Herzog, Darryl A. Knight, Cory M. Hogaboam and Lynne A. Murray, Clusterin Regulates Cell Senescence During Lung Injury and Fibrosis, The J. of Clinical Investigation. Jul 22, 2016 uploaded website.

Sandra Gallagher, Isharat Yusuf, Tom M. McCaughtry, Sean Turman, Hong Sun, Ronald Herbst, and Yue Wang MEDI-551 Treatment Effectively Depletes B Cells and Reduces Serum Titers of Autoantibodies in Sle1-Human CD19 Transgenic Mice. Arthritis Rheumatol 2016, 68: 965-976

Raphaël Lemaire, Timothy Burwell, Hong Sun, Tracy Delaney, Julie Bakken, Lily Cheng, Marlon C. Rebelatto, Meggan Czapiga, Isabelle de-Mendez, Anthony J. Coyle, Ronald Herbst, Robert Lafyatis and Jane Connor Neutralization of the anti-fibrinolytic function of plasminogen activator inhibitor-1 resolves skin fibrosis Arthritis Rheumatol 2016, 68: 473-483

Isharat Yusuf, Jessica Stern, Tom M. McCaughtry, Sandra Gallagher, Hong Sun, Changshou Gao,Thomas Tedder, Gianluca Carlesso, Laura Carter, Ronald Herbst and Yue Wang Germinal Center B Cell Depletion Diminishes CD4+ Follicular T Helper Cells in Autoimmune Mice, PLoS One, 2014 Aug 7; 9(8) e102791

Leao KE, Leao RN, Sun H, Fyffe RE, Walmsley B. Hyperpolarization-activated currents are differentially expressed in mice brainstem auditory nuclei. J Physiol. 2006; 576:849-64.

Leao RN, Sun H, Svahn K, Berntson A, Youssoufian M, Paolini AG, Fyffe RE, Walmsley B.

Topographic organization in the auditory brainstem of juvenile mice is disrupted in congenital deafness. J Physiol. 2006; 571:563-78. (featured as cover illustration of journal)

R. Leao, S. Oleskevish, H. Sun, M. A. Bautista, R.Fyffe and B. Walmsley, Differences in glycinergic mIPCs in the auditory brainstem of congenitally deaf and normal neonatal mice. J.Neurophysiol., 2003, 91: 1006~1021, 2004

H. Sun, R. Leao, M. Bautista, B. Walmsley and R. Fyffe, Topographic arrangement of high threshold voltage dependent potassium channels in the MNTB of normal and congenitally deaf mice. Society for Neuroscience, 2004 San Diego.

H. Sun, R. Fyffe, B. Walmsley, Gephyrin membrane clusters and intracellular aggregates in principal neurons in the mntb of normal and congenitally deaf ( dn/dn ) mice. Society for Neuroscience, 2003, New Orleans.

B. Walmsley, R. Leao, S. Oleskevich, H. Sun, and R. Fyffe, Differences in glycinergic transmission in mntb principalneurons between normal and congenitally deaf ( dn/dn ) mice. Society for Neuroscience, 2003 New Orleans.

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