Polymer synthesis and characterization, Biological characterization

Zhaobo Fan

Address: *** **** ******** **., ***. *07, Columbus, OH 43202;

Tel: 631-***-****;

Email: acwqyn@r.postjobfree.com

OBJECTIVE & HIGHLIGHTS

Seeking a full-time position of synthesis and characterization in functional polymers and other chemistry-related fields.

- 5+ years of experience in the study of polymer formulations and extensive investigation on structure-property-processing relationships.

- Proficiently handle the material preparation: organic synthesis and polymerization independently use the characterization method: 1D NMR, DSC, IR, XRD, UV-Vis, tensile test, and statistical data analysis.

- Biological operation and analysis: cell culture, tissue sectioning, RT-PCR, and confocal microscopy.

RESEARCH EXPERIENCE

Graduate Research Associate, the Ohio State University, Columbus, OH Jul. 2012 – Present

Designed and Prepared the Functional Polymers

- Prepared a novel acrylate polylactide (APLA) based macromer by two-step reaction (Ring-opening and acylation) and copolymerized with NIPAAm and HEMA to obtain thermosensitive APLA hydrogel. Analyzed physical, thermal and mechanical properties so as to understand structure-property relationship.

- Synthesized 2-Nitroimidazolyl based hypoxia sensitive moiety and conjugated onto the side chain of thermosensitive APLA-based hydrogel. Compared its physical and thermal properties under normal and hypoxia to investigate the structure-property relationship.

- Investigated the relationship between modulus of series concentrations of APLA hydrogel and phenotype of cardiac fibroblast, in controlling the cardiac fibrosis in vitro and in vivo after myocardial infarction (MI).

Developed the Thermosensitive Hydrogel Based Drug Delivery System

- Build up a TGF-β receptor II binding peptide (TBP) delivery system and detected the binding affinity between TGFβ and TBP to evaluate its efficacy of reducing cardiac fibrosis in vitro and in vivo.

- Designed a matrix metalloproteinases-2 inhibitor (MMPI) delivery system and evaluated its effect in reducing cardiac fibrosis, increasing vessel density, and improving cardiac function in vitro and in vivo.

- Developed a basic fibroblast growth factor (bFGF) delivery system, and examined its effect in reverting the myofibroblast into quiescent fibroblast in 2D (Surface culture) and 3D (Collagen seeding) models.

- Pioneered two novel dual delivery systems of bFGF/MMPI and TBP/oxygen release microsphere and applied in vitro and in vivo to improve heart function after MI.

Graduate Research Associate, State University of New York at Stony Brook, Stony Brook, NY Aug. 2010-May 2012

- Prepared the triacetylene monomers through “Host-Guest” strategy, and carried out topochemical 1,6- polymerization.

- Prepared the pyridyl substituted triacetylene monomers assembled in the pattern of π-π interaction, and explored their topochemical 1,6- polymerization.

Senior Design, Qingdao University of Science and Technology, Qingdao, SD Sep. 2008-Jun. 2010

- Synthesized a novel anthracene-based compound DSB-DSA (DSB: 1,4-distyryl-benzene; DSA: 9,10-bis (p-t-butylstyryl) anthracene) by Horner-Emmons reaction and Suzuki coupling, which showed aggregation-induced fluorescence emission.

EDUCATION

The Ohio State University, Columbus, OH Jul. 2012 – Aug. 2016 Ph.D. Candidate in Material Science and Engineering; Advisor: Dr. Jianjun Guan

State University of New York at Stony Brook, Stony Brook, NY Aug. 2010 – May. 2012 M.S. in Chemistry; Advisor: Dr. Joseph W. Lauher

Qingdao University of Science & Technology, Qingdao, SD Aug. 2006 – July. 2010 B.E. in Polymer Science & Engineering; Advisor: Dr. Wenjun Yang

PEER REVIEWED PUBLICATIONS

1. *Li, Z.; *Fan, Z.; *Xu, Y.; Lo, W.; Wang, X.; Niu, H.; Li, X.; Xie, X.; Khan, M.; Guan, J. pH-Sensitive and Thermosensitive Hydrogels as Stem-Cell Carriers for Cardiac Therapy. ACS Appl Mater Interfaces. 2016;8(17):10752-60. (* Co-first author)

2. *Li, Z.; *Fan, Z.; *Xu, Y.; Niu, H.; Xie, X.; Liu, Z.; Guan, J. Thermosensitive and highly flexible hydrogel capable of stimulating cardiac differentiation of cardiosphere-derived cells under static and dynamic mechanical training conditions. ACS Appl Mater Interfaces. 2016; 8:15948-57 (* Co-first author)

3. Fan, Z.; Guan, J. Antifibrotic therapies to control cardiac fibrosis. Biomater Res. 2016; 20:13.

4. Li, H.; Duann, P.; Lin, P.H.; Zhao, L.; Fan, Z.; Tan, T.; Zhou, X.; Sun, M.; Fu, M.; Orange, M.; Sermersheim, M.; Ma, H.; He, D.; Steinberg, S.M.; Higgins, R.; Zhu, H.; John, E.; Zeng, C.; Guan, J.; Ma, J. Modulation of wound healing and scar formation by MG53-mediated cell membrane repair. J Biol Chem. 2015;25: 680074.

5. Xu, Y.; Fu, M.; Li, Z.; Fan, Z.; Li, X.; Liu, Y.; Anderson, PM.; Xie, X.; Liu, Z.; Guan, J. A prosurvival and proangiogenic stem cell delivery system to promote ischemic limb regeneration. Acta Biomater. 2016; 31:99-113.

6. Xu, Y.; Li, Z.; Li, X.; Fan, Z.; Liu, Z.; Xie, X.; Guan, J. Regulating Myogenic Differentiation of Mesenchymal Stem Cells Using Thermosensitive Hydrogels. Acta Biomater. 2015;12:30054-4.

7. Zhang, X.; Li, W.; Li, X.; Liu, T.; Fan, Z.; Yang, W. Alternating copolymers of 2,6-Bis(p-dihexylaminostyryl)anthracene-9,10-diyl and N-Octylcarbazole with large two-photon cross sections. J Polym Sci Pol Chem. 2010;48:5704-11.

MANUSCRIPT IN PREPARATION

1. Fan, Z.; Fu, M.; Li, Z.; Lin, P.; Guan J. Controlled Release of TGF-β Receptor II Inhibitor to Control Cardiac Fibrosis.

2. Fan, Z.; Fu, M.; Li, Z.; Li, X.; Guan J. An Injectable and Thermosensitive Hydrogel Capable of Delivering Matrix Metalloproteinases-2 Inhibitor (MMPI) to Control Myocardial Remodeling.

3. Fan, Z.; Xu, Z.; Li, X.; Guan J. An Injectable and Thermosensitive Hydrogel Capable of Delivering Basic Fibroblast Growth Factor (bFGF) to Control Cardiac Fibrosis.

4. Fan, Z.; Fu, M.; Xu, Z.; Li, H.; Li, X.; Guan J. Dual Delivering TGFβ Receptor 2 Binding Peptide (TBP) and Oxygen Release Microsphere (ORM) via Injectable Thermosensitive Hydrogel to Control Cardiac Fibrosis.

5. Fan, Z.; Fu, M.; Li, Z.; Li, H.; Guan J. Dual Delivering Basic Fibroblast Growth Factor (bFGF) and Matrix Metalloproteinases-2 Inhibitor (MMPI) via Injectable Thermosensitive Hydrogel to Control Cardiac Fibrosis.

CONFERENCE ORAL/POSTER PRESENTATIONS

1. Fan, Z.; Fu, M.; Xu, Z.; Li, X.; Guan J. An Injectable and Thermosensitive Hydrogel Capable of Delivering Basic Fibroblast Growth Factor to Control Cardiac Fibrosis. 2016 World Biomaterial Congress, Montreal, QC. May 17-22, 2016. (Oral)

2. Fan, Z.; Fu, M.; Li, Z.; Li, X.; Xu, Y.; Guan J. Controlled Release of TGF-β Receptor II Inhibitor to Control Cardiac Fibrosis. 2015 Institute for Materials Research Conference, Columbus, OH. May. 12-15, 2015. (Poster)

3. Fan, Z.; Fu, M.; Li, Z.; Li, X.; Xu, Y.; Guan J. An Injectable and Thermosensitive Hydrogel Capable of Delivering Matrix Metalloproteinases-2 Inhibitor to Control Myocardial Remodeling. 2015 Society for Biomaterials Annual Meeting, Charlotte, NC. Apr. 15-18, 2015. (Oral)

4. Fan, Z.; Fu, M.; Li, Z.; Li, X.; Xu, Y.; Guan J. Controlled Release of TGF-β Receptor II Inhibitor to Control Cardiac Fibrosis. 2014 Biomedical Engineering Society Annual Meeting, San Antonio, TX. Oct 22-25, 2014. (Oral)

5. Fan, Z.; Hueckel, T.; Lauher, J. W. Host-Guest Approach for 1,6-Topochemical Polymerization of Triacetylenes. 2011 Chemistry Research Day, Stony Brook, NY. Nov. 18th, 2011. (Poster)

MENTORING AND TEACHING EXPERIENCE

- Teaching Assistant for Undergraduate Material Fundamental and Biomaterial Lab course, Spring 2014, 2015, and Autumn 2015. Class size: 15 students per session, 4-5 sessions per week. Each TA semester needs 12 weeks tutoring and grading.

- Mentor for MSE Senior Design, Spring 2014.

- Mentor for Women in Engineering Program, Summer 2012, 2013, and 2014. Entrance level of polymer science lecturing for 40min each year.

AWARDS AND HONORS

- Scholarship of Qingdao University of Science and Technology in 3 consecutive years (2006-2009)

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