CURRICULUM VITAE

Pradeep Alava

**** *********** ****

Apartment 12,

Durham, 27713

North Carolina

Unites States of America

Mobile phone number: 001-409-***-****

E-mail: acwabc@r.postjobfree.com

Date of Birth: 15-04-1984

Work permit: USA Green Card Holder

Profile

I am a PhD degree holder in applied biological sciences with eight years’ experience of research and development and seven years’ experience in quality control lab.

Work experience

January 2013- Present: POST DOC (Environmental protection Agency, RTP, NC, USA)

Working as research scientist at USEPA, Method Development and Application Branch, Research Triangle Park, North Carolina.

Leads and part of quality control branch dealing with testing the raw materials, reporting, and performing different assays.

Prepared and finalized different SOPs’ (standard operation procedures) for different testing methods and to operate different analytical instruments as part of GLP requirements.

Proposed exposure limits to humans by conducting in-vitro and in-vivo experiments to quantify and qualify the bioaccessibility and bioavailability of trace elements, contaminants in environment etc.

Developed and validated different methods as part of GMP requirements.

Validating the in-vitro data and in-vitro methods by comparing it to in-vivo data.

Developed HPLC –ICP-MS method to evaluate arsenic speciation in biological matrices.

Developed digestion methods for total lead and arsenic analysis in environmental and biological matrices.

Carries out analysis of different biological and environmental samples using different analytical tools like HPLC, ICP-MS etc.

Performed purification and separation of different active chemical ingredients after their preparation. Performed assay and analysis on them to qualify and quantify them.

Blood analysis for total lead content.

Deals with reporting all the results after analysis.

Writing publications.

Mentoring trainees.

Framing plans to remediate heavy metal contaminations in environment and their adverse impacts on human health.

Use SAS to sort and organize my research data; like creating tables, graphs.

2008- 2012 October: RESEARCH SCIENTIST (Gent University, Ghent, Belgium)

I have conducted several experiments using SHIME reactor (Simulator of Human Intestinal Microbial Ecology). It is an in-vitro model for human gastrointestinal tract. All in-vitro data was validated against in-vivo metabolic reactions and microbial community data.

Performed different microbiological assays. Screening and quantifying different microbial strains using PCR and qPCR

Developed and improved analytical methods by experimental designing, validating and implementing new tests, methods, instrumentation, Good Laboratory Practice (GLP) programs and procedures.

DMPK assays’.

Analyzing different food and biological samples for arsenic and its speciation as part of quality control.

I have guided two master students for their thesis work.

Developed SOP for different instruments and different technical procedures.

Using basic statistical test like paired test, t test etc for confidence intervals and use of basic SAS program commands to create tables and graphs from the data.

2007-2008 (Catholic university, Leuven, Belgium)

Involved in the analysis of drugs and pharmaceuticals as per monographs of Eur. Ph. especially using techniques like HPLC-UV, HPLC-PED, CE-UV and HPLC-ICPMS, ESI-MS, MS/MS, ICP-OES under GLP requirements.

Developed and validated an alternative assay method for gentamycin sulphate using HPLC-PED, which is now being used in European pharmacopoeia.

Development and validation of analytical methods (ICH guidelines):

Liquid chromatography hyphenated with UV detector, Mass spectrometer, ICP-MS.

Validation of analytical instruments.

DMPK assays’.

Performed purification and separation of different active chemical ingredients and drugs after their preparation. Performed assay and analysis on them to qualify and quantify them.

2005 (Jawaharlal Nehru technological university, Hyderabad, India)

Practical training in India Penicillin’s Limited for a period of six weeks in the production (Good Manufacturing Procedures (GMP)) and quality control department, as part of my final year graduate curriculum.

Academic Record

2008 – 2012

PhD student in the lab of Analytical Chemistry and Applied Ecochemistry, Ghent University, Ghent, Belgium (defense date: October 12, 2012).

Objective of PhD research: To study physical, chemical and microbial effects on arsenic speciation changes in human gastro-intestinal tract. Then followed by studies on the bio-accessibility of arsenic species; both toxic and non-toxic.

2007–2008

Post Graduate in Pharmaceutical Research (attained distinction), Catholic University, Leuven, Belgium.

Project: Collaborative studies for detection of related substances and composition of gentamicin sulphate using Liquid Chromatography with Pulsed Electrochemical Detection (LC-PED).

2002–2006

Bachelor of Pharmacy (attained first class), Jawaharlal Nehru Technological University, Hyderabad, India.

Project: Formulation and in-vitro evaluation of fast dispersible Tinidazole tablets.

Skills

Technical

Separation techniques: Thin layer chromatography (TLC and HPTLC), liquid chromatography (LC), capillary electrochromatography (CEC) and gas chromatography (GC)

Detection techniques: UV, florescence, DAD, PED, ELSD, ICP-MS, ICP-AES and MS

Hyphenated techniques: HPLC-UV, HPLC-ICPMS, HPLC-ESI-MS, CEC-UV

Other analytical techniques: FT-IR, semi micro determination of water and titrimentry

Computer

Operating: Windows 95/98/ME/XP/vista/7

Software: MS Office, ChemWindow®, Modde 4.0®, Origin®, Endnote® Reference Manager® and Microsoft Office® and other internet tools

Multimedia: Adobe Photoshop, page maker

Language (proficiency level)

English (fluent), Telugu (mother tongue)

Statistical knowledge

PCA, ANOVA, SPLUS, SAS

Awards

Attained NRC grant to proceed with my postdoctoral research in USA

Attained CWO travel grant from Gent University to attend International symposium on arsenic.

Best project award in graduation.

FWO travel grant to attend International symposium

Review Activities

Science of the total environment

References

I can provide the details, if needed.

ADDITIONAL INFO

Oral Presentations

Effect of cooking procedures on As species in rice and biotransformation of these species in the human gut.

Pradeep Alava, Tom Van de Wiele, Gijs Du Laing, Katrijn Van den Broeck, Filip Tack.At As 2010, The Third International Conference on As in Environment.

Analysis of As in bio-samples from the human gastrointestinal tract using HPLC-ICP- MS.

Pradeep Alava, Gijs Du Laing, FilipTack, Tom Van de Wiele, at Fourth International conference of Toxic Elements in Food (TEF-4).

Influence of human gut microbiota on biotransformation of arsenic from different rice matrices

Pradeep Alava, Gijs Du Laing, Katrijn Van den Broeck, Filip Tack, Tom Van de Wiele,.At As 2012, The Fourth International Conference on As in Environment, Australia, July 2012.

Poster Presentations

Analysis of As in bio-samples from the human gastrointestinal tract using HPLC-ICP- MS.

Pradeep Alava, Tom Van de Wiele, Gijs Du Laing, FilipTack, at Euro analysis symposium 2009, Innsbruck, Austria.

Effect of cooking procedures on As species in rice and biotransformation of these species in the human gut.

Pradeep Alava, Tom Van de Wiele, Gijs Du Laing, Katrijn Van den Broeck, Filip Tack .At SETAC 2010, Seville, Spain.

HPLC-ICPMS method development to monitor As speciation changes by human gut microbiota.

Pradeep Alava, Tom Van de Wiele, Gijs Du Laing, Katrijn Van den Broeck, Filip Tack, At SETAC 2010, Seville, Spain and At As 2010, The Third International Conference on As in Environment

Effect of diet composition on bio accessibility and colon biotransformation of Arsenic

Pradeep Alava, Gijs Du Laing, Filip Tack, Tom Van de Wiele,.At As 2012, The Fourth International Conference on As in Environment, Australia, July 2012.

Monomethylthioarsenicals are substrates for human arsenic (+3 oxidation state) methyltransferase”.

Pradeep Alava, Karen Bradham National Exposure research laboratory, NERL, RTP, NC, USA. Michael Kohan, David.J.Thomas, NHEERL, RTP, NC, USA. Jana Navratilová, Zuzana Drobná, Miroslav Stýblo, Department of Nutrition, University of North at Chapel Hill, NC, USA.

Publications

Published

HPLC-ICPMS method development to monitor As speciation changes by human gut microbiota.

Alava, P., Tack, F., Laing, G. D. and Van De Wiele, T., Biomedical Chromatography, 2012, 26(4) : 524-533

Extensive grinding and pressurized extraction with water are key points for effective and species preserving extraction of As from rice.

Pradeep Alava, Tom Van de Wiele, Filip Tack, Gijs Du Laing. Analytical Methods, 2012, 4 (5), 1237 – 1243.

As in cooked rice: effect of chemical, enzymatic and microbial processes on bioaccessibility and speciation in the human gastrointestinal tract.

Guo-Xin Sun, Tom Van de Wiele, Pradeep Alava, Filip Tack, Gijs Du Laing. Environmental Pollution, 162 (2012) 241-246

Arsenic undergoes significant speciation changes upon incubation of contaminated rice with human colon microbiota.

Alava, P., Tack, F., Du Laing, G., and Van De Wiele, T., Journal of Hazardous materials (Accepted) http://dx.doi.org/10.1016/j.jhazmat.2012.05.042.

Arsenic bioaccessibility upon gastrointestinal digestion is highly determined by its speciation and lipid-bile salt interactions

Alava, P., Du Laing, G., Tack, F., and Van De Wiele, T., J Environ Sci Health A Tox Hazard Subst. Environ Eng. 2013;48(6):656-65. doi: 10.1080/10934529.2013.732367

Arsenic Thiolation by Sulphate Reducing Bacteria from the Human Gastrointestinal tract

Sergio Rubin, Alava, P., Du Laing, G., Tack, F., and Van De Wiele, T., Environ Health Perspect; DOI:10.1289/ehp.1307759

Westernized diets lower arsenic gastrointestinal bioaccessibility but increase the colon microbial formation of hazardous As species

Alava, P., Du Laing, G., Tack, F., and Van De Wiele, T., Chemosphere. 2015 Jan;119:757-62. doi: 10.1016/j.chemosphere.2014.08.010. Epub 2014 Sep 3.

Additionally one publication as first author and one publication as co-author are under process of review at my mentor desk.

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