Upasana Dutta Linked in:

*** **** ****, *** #**, San Francisco, CA 94132. Mob: 202-***-**** email: acwa6l@r.postjobfree.com Objective

Graduate in Biochemistry and Molecular Biology with experience in quality control and clinical laboratory seeking a position which best suits my interest.

Skills

Molecular Biology & Biochemistry:

Gene and recombinant DNA cloning,

PCR, RT-PCR, qPCR, Southern blot, northern blot, DNA isolation from bacteria.

Agarose Gel Electrophoresis,

Restriction Enzyme digestion

Molecular Cloning, Recombinant protein expression and purification,

Western blot analysis, ELISA testing,

GMP Compliant testing.

Knowledge of Coulter counters.

Strong organizational and troubleshooting skills

Proficient in Microsoft Excel, word, PowerPoint

Knowledge of Cell Quest, Diva. Flow Jo

Cell Biology:

FACS analysis at Georgetown University

Growth cell assays, Primary cell culture,

Mammalian Cell culture, Transfection of cells,

Cell fractionation by differential centrifugation

Preparation of cells for cryopreservation,

Immunofluorescence, multiwall plate-based cytotoxicity and proliferation assays

Instrumentation: Hem cytometer, UV-visible absorption and fluorescence spectroscopy, atomic absorption, co focal, bright field and fluorescence microscopy.

Knowledge of bioinformatics tools such as BLAST, FASTA, and DNA analysis software.

Training and Internships

Diploma in Bioinformatics, Indgen Life Technologies, India (2009)

Certification in HPLC & GC: Operational, Preventive and corrective measures Central Food Technological Research Institute (CFTRI). Mysore, India.

Internship: Scientific analyst at Molecular Connections, Bangalore, India. (2009) Education

Master of Science: Biochemistry & Molecular Biology Graduated: 31 Dec 2015 Georgetown University GPA 3.5/4.0

Master of Science: Biochemistry Graduated: 22 Aug 2009 Bangalore University, India GPA 3.7/4.0

Professional Experience

Internship/Research Associate, Lombardi Cancer Centre, Georgetown University. (May 2015 - Dec 2015)

Regulation of Estrogen receptor- through calcium channel blockers in hormone independent ER positive breast cancer cells, Proficient in handling primary cells, tissue culture and cell based assays.

Also worked on PCR, RT-PCR, qPCR Isolation of RNA, blotting techniques and also mastered the technique of cell cycle assay analysis, intracellular antigens and apoptosis using FACS technology. Biochemist, Dr. Mohan’s Diabetes Center, India. (Jan 2014- Dec 2014)

Involved in performing diagnostic testing of blood and body fluids of patients on biomedical instruments. Lecturer, Biochemistry Department, Vrundavan Institute of Nursing, India. (Jan 2013-Dec 2013)

Involved in the administration of degree and postgraduate courses as well as responsible for organizing lectures and supervising seminars and tutorials.

Quality Control Officer, Biochemistry Department, Tulip Diagnostics (P) Ltd, India. (Jun 2010-Nov 2012)

Maintain compliance with applicable cGMP requirements for QC laboratory.

Worked on Testing, validation and analysis of fully automatic, Random access clinical chemistry Analyzer with throughput 100/200/300 tests per hour, fully automated Hematology analyzer, Electrolyte Analyzer. And recording data in an accurate and timely manner per applicable regulatory and corporate requirements.

Worked in research team and developed expertise in PCR and ELISA testing, preparing culture media, chemicals, reagents and solutions.

Maintaining and testing of Raw Materials, Final products, performing QC analysis as per ISO-13485:2003.

Involved in client complaint handling management of each complaint through root cause and escalating quality non- conformities as appropriate. and conduct troubleshooting analysis. Reference

Available on Request

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