Aditya Ambade

Visa Status – Permanent Resident of the United States

** ******** ******, *********, ** 01604

Email: acvxbl@r.postjobfree.com Phone: 714-***-****

https://www.linkedin.com/in/aditya-ambade-473b7513 Summary:

Scientist with expertise studying the role of chronic inflammation in accelerating cancer progression and also studying immunotherapy and adoptive T cell therapy for solid cancers (i.e. head, neck, & hepatocellular cancer).

Experience performing preclinical testing of small molecule inhibitors in various animal models.

Experience writing grants and peer reviewing papers, and editing colleague’s papers and grants.

Successfully collaborated with cross functional teams and worked with clinicians, scientists, and engineers. Research Experience:

UMass Medical School, Dept. of Medicine Worcester, MA July 2014 - present Post-Doctoral Research Associate; Advisor: Dr. Gyongyi Szabo (Prof. and Associate Dean for Clinical &Translational Sciences)

Study the role of diet induced obesity and non-alcoholic steatohepatitis (NASH) in altering macrophage polarization related to immuno tolerance and cancer progression.

In a team of 4, developed the first animal model proving the role of alcoholic hepatitis in accelerating HCC.

Research resulted in 2 first authored papers; Presented at “The Liver Meeting” in San Francisco, Nov 2015.

Wrote 3 grants (one for the DoD and two for industry sponsored research projects).

Independently reviewed 12 scientific research articles, wrote SOPs / IACUC protocols for routine labwork.

Worked with clinicians to acquire human biopsies and reviewed IRB proposals for studies on human samples.

Led cross-sectional teams with a pharmacologist, neurobiologist, veterinarian, biostatistician and finance person.

Trained 2 MD/PhD fellows, 3 rotation students and 1 junior postdoc. UMass Medical School, Department of Medicine, Worcester, MA October 2009 – June 2014 Post-Doctoral Research fellow; Advisor: Dr. Pranoti Mandrekar

Research focused on developing a potential therapeutic drug (17-DMAG) for alcoholic liver cirrhosis.

Led a team of 4 comprising a research associate, technician and undergraduate student.

Responsible for literature search for grant / manuscript writing, project designing and peer review.

Performed statistical data analysis (GraphPad Prism).

Published 4 papers, 2 review articles and one book chapter. Wrote 2 grants (NIH and DoD).

Trained 2 PhD candidates, 3 summer interns, and 2 research associates. Tata Memorial Centre (TMC), University of Mumbai – India 2003-2008 Doctoral Research, Cancer Research Institute; Advisor: Dr. Rita Mulhekar

Focus on developing a Combination Gene Therapy for Head & Neck Cancer; Lead to 2 papers, 4 presentations.

Achieved 70% tumor regression in head and neck squamous cell carcinoma (HNSCC) xenograft mouse model using a combination of Interleukin-2 and HSV/thymidine kinase-GCV gene therapy.

Collaborated with a virologist, clinical hematology researchers, pathology, head & neck surgery dept’s at TMC.

Successfully wrote/obtained 2 grants (Dept of biotechnology & Indian Council for Medical Research for grad work.

Trained 2 PhD students and one technician during this period. Presented at 2 int’l and 2 national conferences.

Actively involved in interacting with vendors and made decisions about purchasing lab reagents & instruments. Education:

Ph.D., Cancer Biology – Gene Therapy, University of Mumbai – Mumbai India 2008 Diploma, Clinical Research, Catalyst Clinical Services Pvt. Ltd – Delhi, India 2008

Relevant Course Topics:clinical trial overview, ethics & regulatory affairs, IND application/drug development steps Master’s, Biochemistry, University of Pune – Pune, India 2002 Bachelors, Chemistry, University of Pune – Pune, India 2000 Certifications

IACUC certification and Certification in ethical use of human samples and patient data [IRB] protocols Aditya Ambade (714) 317 – 0986 mailto:acvxbl@r.postjobfree.com 2

Key Publications: 10 of 15 (Complete list can be found here):

Aditya Ambade, Abhishek Satishchandran, Gyongyi Szabo (2016) Chronic alcohol accelerates early hepatobiliary cancer by increasing inflammation, stemness, EMT and miR-122 mediated HIF-1α activation. Scientific Reports 6:21340. PMID: 26888602

Ambade A, Satishchandran A, Gyongyosi B, Lowe P, Szabo G (2016) Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease. World J Gastroenterol. 2016 28;22(16):4091-108. doi: 10.3748/wjg.v22.i16.4091. PubMed PMID: 27122661

Michal Ganz, Terence N Bukong, Timea Csak, Banishree Saha, Jin-Kyu Park, Aditya Ambade, Karen Kodys and Gyongyi Szabo (2015) Progression of non-alcoholic steatosis to steatohepatitis and fibrosis parallels cumulative accumulation of danger signals that promote inflammation and liver tumors in a high fat-cholesterol-sugar diet model in mice. Journal of Translational Medicine 13(1):193. DOI: 10.1186/s12967-015-0552-7. PMID: 26077675

Ambade, A., Catalano, D., Lim. A., Kopoyan. A., Shaffer, SA., Mandrekar, P., (2014) Pharmacological Inhibition of Heat Shock Protein 90 Alleviates Steatosis and Macrophage Activation in Murine Acute and Chronic Alcoholic Liver Injury – Journal of Hepatology 61(4):903-11. PMID: 24859453

Ambade, A., Mandrekar, P., (2012) Oxidative stress and inflammation: essential partners in alcoholic liver disease. Int J Hepatol.; 2012:853175. PMID: 22500241

Ambade, A., Catalano, D., Mandrekar, P. (2012) Hsp90 inhibition attenuates pro-inflammatory responses and prevents LPS induced liver injury. Hepatology 55(5):1585-95. PMID: 22105779

Mandrekar P., Ambade A., Szabo G., Catalano D. (2011) An essential role for MCP-1 in alcoholic liver injury: regulation of pro-inflammatory cytokines and hepatic steatosis. Hepatology 54(6):2185-97. PMID: 21826694

Ambade, A. Joshi, G. V. and Mulherkar, R. (2010) Effect of suicide gene therapy in combination with Immunotherapy on antitumour immune response and tumour regression in a xenograft mouse model for Head and Neck Squamous cell carcinoma. Indian J Med Res. 132 (October):415-22. PMID:20966520

Li, H., Ambade, A., and Re, F. (2009) Cutting Edge: Necrosis Activates the NLRP3 Inflammasome. The Journal of Immunology 183:1528-1532. PMID:19596994

Ambade, A., and Mulherkar, R. (2008) Adoptive T cell transfer augments IL-2 mediated tumor regression in a HNSCC xenograft nude mouse model. Cancer Letters 272(2):316-324. PMID:18762367 Selected Conference Presentations: (5 out of 34)

“Myeloid specific deficiency of gp96, a master chaperone of toll-like receptor 4 (TLR4), reduces inflammatory cytokines and protects against alcoholic liver injury” at American Association for Study of Liver Diseases

[AASLD], The Liver Meeting 2013, Washington, DC, November 2013 (Oral Presentation)

“HSF1 deficiency exacerbates liver fibrosis: regulation of inflammatory cytokines and fibrogenesis” at the annual meeting of Cell Stress Society international [CSSI], Alexandria, VA, November 2012 (Oral Presentation)

“HSP90, A potential therapeutic target in alcoholic liver disease” at 35th annual meeting of Research Society on Alcoholism [RSA], San Francisco, CA, June 2012 (Oral Presentation)

“Inhibition of Hsp90 attenuates chronic alcohol induced proinflammatory cytokine TNF in Kupffer cells: role for molecular chaperones”. Alcohol and Immunology Research Interest Group [AIRIG] meeting, Maywood, IL, November 2010 (Travel grant for oral and poster presentation)

“Combination Gene therapy for Head and Neck Cancer” at the International Society for Cell & Gene Therapy conference [ISCGT], Mumbai, India, November 2007 (Oral and Poster presentation)

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