James D. Marion Jr., Ph.D.

*** ******* ****, *********, ** 07450

***** **** ***** ****, *** Diego, CA 92126

Cell: 201-***-**** E-mail: acvs29@r.postjobfree.com SUMMARY

A results-driven research scientist with more than 10 years of laboratory experience in an academic setting. Highly proficient in techniques spanning multiple scientific fields including, but not limited to, enzymology, immunology, oncology, and biophysics. Outstanding verbal and written communication skills with invited authorship in the Encyclopedia of Molecular Life Sciences, publications in a range of peer-reviewed scientific journals, and over 30 presentations at national conferences. Demonstrated skill in managing, mentoring, and training scientific staff at all levels of expertise.

AREAS OF STUDY

Enzymology Immunology Biophysics Cancer immunotherapy Innate-immunity Protein-protein interactions

Signal transduction Molecular and structural biology EXPERTISE

PCR Protein expression and purification Enzyme kinetics Fast protein liquid chromatography (FPLC) (AKTA systems) High-performance liquid chromatography (HPLC) MALDI-TOF mass spectrometry (Bruker Daltonics Omniflex) Hydrogen-deuterium exchange mass spectrometry Isothermal titration calorimetry (MicroCal iTC200) Dynamic light scattering (Wyatt Technology miniDAWN Tristar Laser Photometer with Quasi-Elastic Light Scattering attachment) Differential scanning calorimetry (MicroCal VP-DSC) Circular dichroism (Jasco J-815 spectropolarimeter with thermal denaturation attachment, Aviv 202) Radioactive kinase assays (ATP, [γ-32P]) Fluorescence spectroscopy (including FRET) Fluorescence anisotropy Small angle x-ray scattering X-ray crystallography (screens and crystal growth (hanging-drop and sitting-drop crystallization)) dsRNA synthesis Mammalian cell culture (HEK293, RAW264.7, PBMCs, OVCAR-3, CAOV-3, SKOV-3, DOV-13) Cell transfection Western blotting Immunoprecipitations (GST, FLAG, 6x-His) Co-immunoprecipitations EXPERIENCE

Post-doctoral Research Fellow September 2013 – May 2016 Elizabeth A. Komives, Ph.D., Laboratory, UC San Diego, La Jolla, CA

Developed in vitro affinity purification and fluorescence anisotropy assays (thiol reactive Oregon Green 488 maleimide) to successfully quantify the dissociation rates of and rates of exchange between specific NFκB dimers with and without IκB proteins present (presented findings at two national conferences (See curriculum vitae) and three manuscripts are in preparation).

Developed and instituted the use of NFκB proteins (N-terminal cysteine constructs, p50/p50 constructs, and cRel/cRel constructs) and IκB proteins (IκBβ constructs and IκBε constructs) that have led to manuscripts in preparation.

Invited to and authored seven chapters in the Encyclopedia of Molecular Life Sciences, an 11-volume work commissioned by the NIH and published through a partnership between “Springer Sciences + Business Media” and the “American Society for Biochemistry and Molecular Biology.”

Used novel research projects to introduce high school students to and train undergraduate/graduate students and researchers in molecular biology (PCR, site-directed mutagenesis), protein expression and purification, and biophysical techniques (ITC, anisotropy, circular dichroism, size exclusion chromatography complexing, etc that led to manuscripts in preparation.

Graduate Student / Doctoral Candidate August 2008 – August 2013 Jessica K. Bell, Ph.D., Laboratory, Virginia Commonwealth University, Richmond, VA

Biophysically characterized a previously discovered inhibitor of the type I interferon response and re-defined it as a novel substrate of TANK binding kinase 1 with multiple roles in the cell (Marion, J.D., et al., 2013).

Collaborated with numerous groups, both inside and outside of the institution, that resulted in presentations at national meetings (see CV) and publications (Mallory, K., et al., 2015, Van, D.N., et al., 2012, Luo, J., et al., 2012).

Awarded membership into two national honor societies (see CV), received a Phi Kappa Phi national scholarship, received multiple travel awards to national conferences (see CV), and awarded a Protein Society Young Investigator Award at the 27th annual national meeting (invited lecture) all in recognition of research excellence.

Used novel research projects to train graduate students and researchers in dsRNA synthesis, mammalian cell culture, immunoprecipitations, western blot analysis, radioactive kinase assays (ATP, [γ-32P]), protein expression and purification, and biophysical techniques (ITC, DSC DLS, mass spectrometry, etc the results of which led to presentations at national meetings (see CV) and publications (in preparation). Staff Scientist / Lab Manager June 2006 – July 2008 J. Ellis Bell, Ph.D., Laboratory, University of Richmond, Richmond, VA

Developed a novel technique to quantify changes in protein/protein or subunit/subunit interaction stability and strength as a consequence of ligand induced changes in quaternary structure (Marion, J.D., et al., 2010).

Developed protocols for and instituted the use of four instruments in the laboratory; MALDI-TOF mass spectrometer (Bruker Daltonics Omniflex), isothermal titration calorimeter (MicroCal iTC200), dynamic light scattering instrument (Wyatt Technology miniDAWN Tristar Laser Photometer with Quasi-Elastic Light Scattering attachment), and differential scanning calorimeter (MicroCal VP-DSC).

Developed protocols for and instituted the use of three techniques in the laboratory; hydrogen-deuterium exchange mass spectrometry, limited proteolysis in combination with mass spectrometry, and the quantification of ligand induced changes of subunit/subunit interactions via a novel dynamic light scattering approach.

Used novel research projects to train undergraduate students in molecular biology (site-directed mutagenesis, etc protein expression and purification (E. coli expression system), and biophysical techniques (fluorescence, kinetics, calorimetry (ITC, DSC), mass spectrometry, circular dichroism, etc that led to presentations at national conferences (see CV) and publications (Bailey, J., Sinanan, L., et al., 2011, Wacker, S.A., et al., 2010). PEER REVIEWED PUBLICATIONS

1. Marion, J.D., Roberts, C.F., Call, R.J., Forbes, J.L., Nelson, K.T., Bell, J.E., Bell, J.K. (2013). Mechanism of endogenous regulation of the type I interferon response by Suppressor of IKKε (SIKE), a novel substrate of TANK binding kinase 1 (TBK1). J. Biol. Chem. 288(25): 18612-23. 2. Luo, J., Obmolova, G., Malia, T.J., Wu, S.J., Duffy, K., Marion, J.D., Bell, J.K., Ge, P., Zhou, Z.H., Teplyakov, A., Zhao, Y., Lamb, R., Jordan, J., San Mateo, L., Sweet, R.W., and Gilliland, G.L. (2012). Lateral clustering of TLR3:dsRNA signaling units revealed by TLR3ecd:3FABS quaternary structure. J. Mol. Biol. 421 (1): 112-24. 3. Van, D.N., Roberts, C.F., Marion, J.D., Lepine, S., Kuzhuvelil, H.B., Schreiter, J., Dumur, C.I., Fang, X., Spiegel, S., and Bell, J.K. (2012). Innate immune agonist, dsRNA, induces apoptosis in ovarian cancer cells and enhances the potency of cytotoxic chemotherapeutics. FASEB J. (8): 3188-98. 4. Marion, J.D., Van, D.N., Bell, J.E., Bell, J.K. (2010). Measuring the effect of ligand binding on the interface stability of multimeric proteins using dynamic light scattering. Anal. Biochem. 407(2): 278-80. 5. Wacker, S.A., Bradley, M.J., Marion, J., Bell, J. (2010). Ligand-induced changes in the conformational stability and flexibility of glutamate dehydrogenase and their role in catalysis and regulation. Protein Sci. 19(10): 1820-9. SUBMITTED PUBLICATIONS

1. Mallory, K., Marion, J.D., Kostick, J.D., Miller, D.P., Bell, J.K., Marconi, R.T. (2016). Molecular dynamics of the interaction of c-di-GMP with the PlzA and PlzC proteins of the Lyme disease and relapsing fever spirochetes. Mol. Microbiol.

PUBLICATIONS IN PREPARATION

1. Marion, J.D., Dembinski, H., Ramsey, K.M., Hoffmann, A., Komives, E.K. (2016). Defining an underlying mechanism that modulates the preferential formation of specific NFκB dimers during cell signaling. 2. Marion, J.D., Ramsey, K.M., Hoffmann, A., Komives, E.K. (2016). Examining the NFκB protein cRel and IκB protein IkBε as they pertain to the NFκB:IκB chaperone landscape. 3. Ramsey, K.M., Marion, J.D., Dembinski, H., Komives, E.K. (2016). The structural and physical characterization of IκBε and its role on the NFκB family.

BOOK CHAPTERS

1. Marion, J.D. (2015). Toll-like receptors: pathogen recognition and signaling. In: Bell, J., Bond, J., Klinman, J., Masters, B., Wells, R. (eds). Molecular life sciences: an encyclopedic reference. Springer reference

(www.springerreference.com). DOI: 10.1007/978-1-4614-6436-5_360-1. 2. Marion, J.D. (2015). Pattern recognition receptors: evolution, redundancy and cross talk. In: Bell, J., Bond, J., Klinman, J., Masters, B., Wells, R. (eds). Molecular life sciences: an encyclopedic reference. Springer reference

(www.springerreference.com). DOI: 10.1007/978-1-4614-6436-5_369-1. 3. Marion, J.D. (2015). TANK-binding kinase 1 (TBK1): structure, function and regulation. In: Bell, J., Bond, J., Klinman, J., Masters, B., Wells, R. (eds). Molecular life sciences: an encyclopedic reference. Springer reference

(www.springerreference.com). DOI: 10.1007/978-1-4614-6436-5_374-1. 4. Marion, J.D. (2015). SIKE: discovery, structure and function. In: Bell, J., Bond, J., Klinman, J., Masters, B., Wells, R. (eds). Molecular life sciences: an encyclopedic reference. Springer reference (www.springerreference.com). DOI: 10.1007/978-1-4614-6436-5_458-1.

5. Marion, J.D. (2015). Immunology and the immune response. In: Bell, J., Bond, J., Klinman, J., Masters, B., Wells, R.

(eds). Molecular life sciences: an encyclopedic reference. Springer reference (www.springerreference.com). DOI: 10.1007/978-1-4614-6436-5_648-1.

6. Marion, J.D. (2015). Toll-like receptor 3: structure and function. In: Bell, J., Bond, J., Klinman, J., Masters, B., Wells, R. (eds). Molecular life sciences: an encyclopedic reference. Springer reference (www.springerreference.com). DOI: 10.1007/978-1-4614-6436-5_772-1.

7. Marion, J.D. (2015). Toll-like receptors: evolution and structure. In: Bell, J., Bond, J., Klinman, J., Masters, B., Wells, R. (eds). Molecular life sciences: an encyclopedic reference. Springer reference (www.springerreference.com). DOI: 10.1007/978-1-4614-6436-5_816-1.

CONFERENCE POSTERS AND PRESENTATIONS

See Curriculum Vitae (more than 30 presentations at national conferences) PROFESSIONAL SOCIETY MEMBERSHIPS

American Society for Biochemistry and Molecular Biology Protein Society Biophysical Society Phi Kappa Phi National Honor Society Alpha Epsilon Lambda National Honor Society EDUCATION

Ph.D. Biochemistry and Molecular Biology June 2013 Virginia Commonwealth University, Richmond, VA

B.S. Biochemistry and Molecular Biology, B.A. History, B.A. Journalism June 2006 University of Richmond, Richmond, VA

Ridgewood High School June 2002

Ridgewood, NJ

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