AARON BRUNO

**** *** ***** **

San Jose, CA

408-***-****

acv83g@r.postjobfree.com

EDUCATION:

June 2004 University of Illinois at Chicago

December 2007 BS Biology

August 2002- University of Connecticut

May 2004 Storrs, CT

Sophomore Standing

Major: B.S. in Biology

EMPLOYMENT:

May 2013- Medimmune, Inc.

July 2016 Mountain View, CA

Laboratory Technician,

Responsible for monitoring stock levels and

Prepared routine lab reagents, buffers, tissue culture materials and any other solutions, requested by end users, via Lab Services Media Request SharePoint

Performed Aseptic cell culture techniques required for monitoring stock levels and subsequent aliquot preps of Research group's specific reagents

Performed autoclaving/sterilization of dry and liquid materials as requested by end users

Monitored, replaced and ordered gases for the site. Ordered, monitored levels and replaced LN2 for cryo freezers

Ordered general lab supplies and chemicals for lab groups

Restocked laboratories with consumables

Collected pipettes due for calibration and sending out for calibration on a monthly basis

Collected dirty glassware from labs, wash and sterilize and redistribute to satellite storage areas.

Received, processed and catalogued samples from various worldwide locations

Performed troubleshooting on lab techniques and processes, including maxi/midi preps and ELISA assays

August 2012- Eurofins STA

October 2012 Gilroy, CA

Lab technician, Plant Health Services

Performed tasks in a client-based laboratory

Performed and documented ELISA data for 15 viruses

Performed RT-PCR RNA to cDNA

Ran and documented Gel Electrophoresis data

Maintained ELISA lab supplies, documentation and workspace

Performed specimen collection from plant samples

Used Excel to generate data

October 2011- Life Technologies, Inc.

December 2011 Foster City, CA

Research Technician; Validation and Verification Team

Performed tasks independently and as a member of the team in an industry setting

Organized and followed protocols and schedules and deadlines

Set up and ran plates for the 3130, 3500 and 310 ABI Capillary Electrophoresis systems

Performed Trouble-shooting and independently maintenance on ABI equipment March 2009- University of Illinois Chicago

March 2010 Chicago, IL

Research Assistant Department of Pharmacology

Developed protocols for mouse monocyte extraction from lung

In charge of mouse genotyping with gel electrophoresis

Performed transformation and purification of plasmid DNA

Placed in charge of purchasing lab supplies and reagents.

Placed inn charge of all paperwork in the lab pertaining to orders

Performed Fragment analysis using Genemapper 4.1

Archived and organized plasmid DNA samples

September 2006- University of Illinois Chicago

March 2010 Chicago, IL

Laboratory Assistant DNA Services

Research Resources Center

In charge of project to extract RNA from whole blood samples

Involved in Helicobacter project, extracting and purifying Helicobacter DNA from human and mouse stool samples

Performed extraction of DNA from feces

Performed PCR on Nucleic Acid

Experience in DNA Sequencing of customer samples

Experience in growing, plating and midi prepping Ecoli

Initiate the organization of lab plasmid stocks

Made and ran ETBR gels

Performed PCR cleanup

Performed Plasmid DNA preps on bacterial cultures

Maintained buffer stocks

Worked with the ABI 3500XL and 3730 capillary electrophoresis machines including maintenance

Worked alongside customers and clients to complete tasks and projects June 2004- University of Illinois at Chicago

August 2006 Chicago, IL

Laboratory Assistant for the Department of

Hematology/Oncology

Grew, fed and split MCF70 cells

Helped customers with running Flow Cytometry

Performed maintenance on FACS sorter

HONORS AND Maryland Schools Certificate of Merit

AWARDS Advanced Placement Scholarship

PUBLICATIONS

Nunez R, Garay N, Bruno A, Villafane C, Bruno E, Filgueira L. Functional and structural characterization of two populations of human monocyte-derived dendritic cells.

Exp Mol Pathol. 2004 Oct;77(2):104-15.

PMID: 15351233 [PubMed - indexed for MEDLINE]

Nunez R, Garay N, Villafane C, Bruno A, Lindgren V., Description of a flow cytometry approach based on SYBR-14 staining for the assessment of DNA content, cell cycle analysis, and sorting of living normal and neoplastic cells. Exp Mol Pathol. 2004 Feb;76(1):29-36.

PMID: 14738866 [PubMed - indexed for MEDLINE]

REFERENCES:

1.Mingjiang Xu MD, PhD., acv83g@r.postjobfree.com 212-***-**** 2.Annette Meyer acv83g@r.postjobfree.com 312-***-**** 3.Ryon Bordoni acv83g@r.postjobfree.com

4.Lachan Band acv83g@r.postjobfree.com

5.Zaida Morales acv83g@r.postjobfree.com

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