Vladislav Bravman

(***) *** – **** acuw0c@r.postjobfree.com

Seattle, WA

Core Qualifications:

Good at problem solving

High attention to detail

Fast learner

Well versed in various laboratory techniques

Skills:

PCR, DNA recovery, gel electrophoresis, SDS-PAGE, protein purification, plasmid purification, Ligation, western blot, ELISA, nanodrop, spectrometer, sterile technique, cell culture, media prep., growing cultures.

Education:

Bachelor's of Science in Biology Graduated March 2016

University of Washington Bothell

Scientific coursework: Biochemistry, Science Methods and Practice, Genetics, Anatomy & Physiology, Undergraduate Research, Evolution, Ecology.

Associate's Degree as Biotech Lab Specialist

Shoreline Community College

Scientific coursework: Molecular Biology, Immunology, Cell Culture, protein purification, solution and media prep. (2 lab classes), general Biology sequence (3 classes with labs), general Chemistry sequence (3 classes with labs), Microbiology.

Laboratory Work Experience:

Chemistry lab assistant September 2013 – December 2013

Shoreline Community College

Skills acquired: preparing solutions, cleaning and setting up lab supplies

Biotech lab specialist program September 2013 – August 2014

Shoreline Community College

Skills acquired: PCR, DNA recovery, gel electrophoresis, SDS-PAGE, protein purification, western blot, ELISA, sterile technique, cell culture, media prep., nanodrop, spectrometer.

Research Experience:

Undergraduate Research September 2015 – March 2016

University of Washington Bothell

Project 1 – Drug Discovery (dry lab)

Successfully discovered novel ligands to act as competitive inhibitors for the 1GTV enzyme in Mycobacterium tuberculosis, using various protein databases and online analysis servers.

Project2 – coevolution in microbes (wet lab)

Grew cocultures of archea and bacteria for varying amounts of generations.

Separated the archea from the bacteria in the cocultures by using different antibiotics to produce monocultures of the species.

Recombined the archea and bacteria into various combinations and recorded their growth until they reached the stationary phase of their growth cycle.

Used anaerobic sterile techniques to reduce exposure to oxygen as much as possible, which included the use of syringes and nitrogen gas to expel any oxygen in the syringe before use

Used Excel extensively to analyze the data gathered from the experiment.

Project3 – molecular dynamics (dry lab)

Performed analysis of the genetic mutations during the evolution of the cocultures containing archea and bacteria.

Used a program called MobaXterm, which used Linux command lines to run scripts, which would perform various analysis procedures on the genomic sequences that were recovered after sequencing each culture, and used Excel to analyze the data output.

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