Jake G. Hoekstra, Ph. D.

425-***-**** • acus4y@r.postjobfree.com

SUMMARY

8+ years experience of successfully developing, designing, implementing, and leading research projects identifying signatures of diseases and targets of therapy

Extensive experience in protocol development, gathering and analyzing data, and communication of results in the form of written and oral reports

Regularly attend group meetings, contribute to collaborative projects, and maintain positive interactions with internal and external groups to achieve research goals

Successfully managed multiple ongoing research projects RELEVANT WORK EXPERIENCE

University of Washington School of Medicine Seattle, WA Department of Pathology 2014-Present

Post-Doc

Leading research projects identifying rare and low frequency mitochondrial DNA mutations and DNA damage in neurodegenerative diseases using a state of the art highly sensitive version of Next Generation Sequencing

Developing protocols, methods, techniques, and quality control measures to improve the quality and efficiency of DNA library preparation and Next Generation Sequencing

Extensive data gathering, analysis with various programs, and presentation

Developing strategies to improve isolation of target DNA

Leading research efforts to identify mechanisms of mitochondrial dysfunction associated with nueordegenerative diseases

University of Washington School of Medicine Seattle, WA Department of Pathology 2008-2014

Graduate Research Assistant

Studied protein changes and cellular mechanisms of neurodegeneration associated with Parkinson’s disease with the goal of identifying novel targets of therapy

Studied the toxicities and contributions of environmental contaminants and elevated neurotransmitters towards neurodegeneration in Parkinson’s disease with a focus on gene- environment interactions

Extensive data gathering, analysis with various programs, and presentation

Designed immunohistochemical methods to identify and characterize cellular protein changes in human tissue related to Parkinson’s disease and individuals exposed to occupational contaminants; modeled changes in primary and immortalized cells in vitro to identify cellular mechanisms of neurodegeneration that proceeded through these changes

Developed procedures to assess cellular morphology, health, and integrity after exposure to particular toxicants or after alteration of protein expression

Utilized several microscopy based techniques, including confocal, fluorescent, and live cell ion imaging, to study changes in cellular function associated with in vitro models

Developed methods and strategies that were critical towards obtaining and analyzing data including protein isolation, protein knockdown, and plate based metrics of cell viability and Jake G. Hoekstra, Ph.D.

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function

University of Washington Seattle, WA

Department of Pathology 2006-2008

Student Research Position

Identification of proteins and protein interactions potentially relevant to Parkinson’s disease pathogenesis

Presented results to senior scientists

Benaroya Research Institute Seattle, WA

2005

Student Research Position

Identification of DNA markers associated with diabetes using real-time PCR

Presented results to senior scientists

EDUCATION

University of Washington School of Medicine Seattle, WA Department of Pathology

Ph.D. in Pathology 2008-2013

University of Washington Seattle, WA

B.S. Molecular, Cellular, and Developmental Biology 2004-2008 Cum Laude

COMMUNITY OUTREACH

2012 Lab tour and demonstration of research for high school English as a Second Language students, Harborview Medical Center, University of Washington 2015-present Demonstration of research for high school students. University of Washington MENTORING/TEACHING

2008-2014 Mentored 1-2 undergraduates and/or graduate students per school year in basic lab techniques, as well as concepts behind ongoing projects 2008-present Technical training of new lab members and colleagues in how to perform techniques used in the lab

AWARDS

2010-2013 NIEHS Environmental Toxicology/Pathology Training Grant (T32 ES007032) PUBLICATIONS

Hoekstra, J.G., Hipp, M.J., Montine, T.J, Kennedy, S.R. Increased Somatic Mutation Load in Mitochondrial DNA is Associated with Pre-Clinical Alsheimer’s Disease. (2016; Manuscript under review at Annals of Neurology).

Cook, T.J., Hoekstra, J.G., Stewart, T., Canales, K.K., Ho, P., Salvador, A.A., Gonzalez-Cuyer, L.F., Nelson, G., Racette, B.A., Checkoway, H., Eaton, D.L., Zhang, J. Astroglial Mortalin is Critical in Manganese-induced Neurotoxiciy. (2016; Manuscript under review at Glia). Jake G. Hoekstra, Ph.D.

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Hoekstra, J.G., Salk, J.J., Kennedy, S.R. Mosaicisms in Human Disease. (2016, Manuscript under preparation).

Samstag, C., Hoekstra J.G., Kennedy, S.R., Youle, R.J., Pallanck, L.J. Accumulation of Mitochondrial DNA Mutations affect age and lifespan in Drosophila Melanogaster. (2016; Manuscript under preparation).

Huber, B.R., Meabon, J.S., Hoffer, Z.S., Zhang, J., Hoekstra, J.G., Pagulayan, K.F., McMillan, P.J., Mayer, C.L., Banks, W.A., Kraemer, B.C., Raskind, M.A., McGavern, D.B., Peskind, E.R., Cook, D.G. Blast Exposure Causes Dynamic Microglial/Macrophage Responses and Microdomains of Brain Microvessel Dysfunction. Jan 14 (2016). Pickrell, A.M., Huang, C., Kennedy, S.R., Ordureau, A., Sideris, D.P., Hoekstra, J.G., Harper, J.W., Youle, R.J. Endogenous Parkin preserves dopaminergic substantia nigral neurons following mitochondrial DNA mutagenic stress. Neuron. Jul 15;87 (37-81). (2015). Hoekstra, J.G., Cook, T.J., Stewart, T., Mattison, H., Dreisbach, M. Hoffer, Z., Zhang, J. Astrocytic dynamin-like protein 1 regulates neuronal survival in Parkinson disease. American Journal of Pathology. Feb. 15, 185(2): 536-49 (2015). Jian, P., Hoekstra J., Heng, X., Kang, W., Ding, J., Liu, J., Chen, S., Zhang, J. P2X7 Receptor is Critical in -synuclein-mediated microglial NADPH Oxidase Activation. Neurobiology of Aging. Jul. 36(7):2304-18. (2015).

Cook, T.J., Hoekstra, J.G., Eaton, D.L., Zhang, J. Mortalin is Expressed by Astrocytes and Decreased in the Midbrain of Parkinson’s disease patients. Brain Pathology. Jun 12 (2015). Wang, J., Hoekstra, J.G., Zuo, C., Cook, T.J., Zhang, J. Biomarkers of Parkinson’s disease: current status and future. Drug Discovery Today 18, 3/4 (2013). Jin, J., Cook, T.J., Hoekstra, J.G., & Zhang, J. “Mortalin Biology: Life, Stress and Death” Ch9. Mortalin in Neurological Diseases. S. Kaul, R.Wadhwa (eds.), © Springer Science+Business Media B.V. (2012)

Hoekstra, J. G., Montine, K. S., Zhang, J. & Montine, T. J. Mitochondrial therapeutics in Alzheimer's disease and Parkinson's disease. Alzheimers Res Ther 3, 21 (2011). Caudle, W. M., Pan, S., Shi, M., Quinn, T., Hoekstra, J., Beyer, R. P., Montine, T. J. & Zhang, J. Proteomic identification of proteins in the human brain: Towards a more comprehensive understanding of neurodegenerative disease. Proteomics Clin Appl 2, 1484-1497 (2008). PRESENTATIONS

Oral Presentations

Jake G. Hoekstra, Ph.D.

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“Astrocytic Dynamin Like Protein 1 in Parkinson Disease.” Mitochondria and Metabolism Interest Group, University of Washington Medicine, South Lake Union, University of Washington, Seattle, WA, 2013.

“Dynamin Like Protein 1 Affects Astrocyte Calcium Response to Glutamate.” Department of Pathology Student Retreat, Department of Pathology, University of Washington, Seattle, WA, 2013.

“Dynamin Like Protein 1 is Decreased in Parkinson’s Disease and Affects Neuron Function.” Department of Neuropathology Research in Progress Seminar, Harborview Medical Center, University of Washington, Seattle, WA, 2012.

“Dynamin Like Protein 1 is Decreased in Astrocytes in Parkinson Disease and Affects Glutamate Transporter Function.” Department of Pathology Student Retreat, Department of Pathology, University of Washington, Seattle, WA, 2012.

“Astrocytic Dlp1 Exerts Protective Effects on Dopaminergic Neurons.” Environmental Pathology and Toxicology Training Grant Retreat, University of Washington Medicine, South Lake Union, University of Washington, Seattle, WA, 2011.

.”Decreased Expression of Dynamin Like Protein 1 Affects Vulnerability of Dopaminergic Cells to Rotenone.” Department of Pathology Student Retreat, Department of Pathology, University of Washington, Seattle, WA, 2011.

Posters Presentations

Hoekstra JG, Cook TJ, Mattison H, Dreisbach M, Zhang J. Dynamin Like Protein 1 is Decreased in Astrocytes Parkinson’s Disease and Affects Glutamate Transporter Function. Environmental Pathology and Toxicology Training Grant Retreat and Department of Pathology Annual Retreat, University of Washington, Seattle, WA, 2013

Hoekstra JG, Cook TJ, Armaly J, Zhang J. Dynamin Like Protein 1 is Decreased in Dopaminergic Neurons in Parkinson’s Disease and Plays a Role in Vulnerability to Rotenone. Environmental Pathology and Toxicology Training Grant Retreat, University of Washington, Seattle, WA, 2011.

Cook TJ, Hoekstra JG, Canales KK, Ho P, Gonzalez-Cuyer LF, Racette BA, Eaton DL, Zhang J. Pathogenesis of mortalin in manganese-induced parkinsoniam. Department of Environmental and Occupational Health Sciences Student Research Day and Department of Pathology Annual Retreat, University of Washington, Seattle, WA, 2014.

Cook TJ, Hoekstra JG, Checkoway H, Eaton DL, Zhang J. Interaction between Parkinson- disease related genes and manganese exposure in astroglial-mediated mechanism of neurodegeneration Department of Environmental and Occupational Health Sciences Student Research, University of Washington, Seattle, WA, 2013.

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