Woan-Nu Yao

acugav@r.postjobfree.com 425-***-****

**** ***** ** ** **** 2 Redmond, WA 98052

EDUCATION

University of Washington, 2006-2009

-Bachelor of Science: Chemistry-Undergraduate research in Dr. Mao’s Lab (2007-2009)

-Minor: Mathematics-experienced in R and MATLAB

EXPERIENCE

Seattle Children’s Research Institute, Seattle, WA

Center for Developmental Biology and Regenerative Medicine

Research Technician III, October 2015 - Present

Research Technician II, May 2011 – September 2015

SKILLS AND TECHNIQUES

-Main responsibilities are independently and successfully multi-tasking numerous molecular biology assays, supporting Lab Director and Senior Research Scientists as a part of team effort to understand complex biological problems

-Molecular biology techniques include competent cell preparation, transformation, plasmid prep, DNA/RNA extraction, transfection, PCR, and gel electrophoresis

-Troubleshooting ability proven by ability to perform multiple molecular biology techniques using both manual method and kits, showing in-depth understanding of the basic principles

-Experience with immunohistochemistry and immunocytochemistry

-Collect and assess image data using bright-field, fluorescence (Lecia DMI600B Inverted and DM40000B Fluorescent Upright), and confocal microscopes (Leica TCS SP5)

-Histology techniques include tissue processing (Tissue-Tek VIP series), paraffin-embedding, cryoembedding, and microtome and cryostat (Leica CM3050, motorized) operation

-Experience with mouse models

-Mouse techniques include colony maintenance (30+ lines), genotyping using PCR or RFLP based methods, and sample collection and management

-Routinely and successfully performe adult and embryonic rodent dissections to collect tissues for RNA extraction and primary cell isolation

-Experience with mammalian cell culture with excellent sterile techniques

-Experience with Flow Cytometry (BD LSR2)

LAB MANAGEMENT RESPONSIBILITIES

-Lead laboratory regulatory compliance

-Review and constantly update experimental protocol and SOP to increase productivity

-Train and supervise junior Research Technicians in molecular biology, histology, microscopy, and mouse work techniques

-Purchasing laboratory supplies and reagents

Covance, Seattle, WA

Medical Technologist II, Operation, September 2013-October 2013

-Successfully performed DNA/RNA quality test using Agilent BioAnalyzer

-Experienced in procedures such as: tissue homogenization, RNA extraction and isolation, reagent preparation, gel electrophoresis, amplification, gel electrophoresis in support of Gene Expression or Genotyping experiments.

-Performed data entry; retrieving, storing and analyzing data from LIMS database ad spreadsheets.

-Worked in a fast-paced, high throughput environment; using liquid handling instrument in accordance with standard operating procedures in a controlled, documented and timely manner.

Diagnos-Tech, INC, Kent, WA

Laboratory Technician, September 2009 – April 2011

-Performed, analyzed, and troubleshooted ELISA experiments

-Prepared reagents, buffers, standards, and controls for testing

-Developed and updated SOP’s following GLP and GCP standards

-Managed maintenance of automated liquid handling system such as Hamilton

-Assisted sample processing department by processing specimens and assigning the specimens with appropriate test

-Ensured all lab supplies are available in work area, and managed laboratory inventory

-Trained new laboratory technicians in many laboratory procedures

University of Washington Department of Pharmaceutics, Seattle, WA

Laboratory Technician, June 2007 – September 2009

-Conducted 96-well plate based protein assay

-Performed molecular biology techniques including subcloning, PCR, and gel electrophoresis

-Assisted senior scientists with delicate in-vitro transwell cell migration assay for the study of drug transport and drug-drug interactions

-Maintained cell cultures for several cell lines including MDCKII and HEK-293

-Supported senior scientists in sample preparation for Flow Cytometry

-Successfully executed efflux assays using lysergic acid diethylamide

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