Post Job Free

Resume

Sign in

University Research

Location:
Memphis, TN
Posted:
May 31, 2016

Contact this candidate

Resume:

Shahzad A. Siddiqi

*** ***** ******, ***** # *-**0, Memphis, TN-38163

acu00e@r.postjobfree.com; Phone # 901-***-****; Fax # 901-***-**** ANALYTICAL & BIOCHEMICAL SCIENTIST

Analytical and Protein biochemist with 15 years of experience at top ranked universities. Highly efficient and motivated analytical and biochemical scientist with demonstrated experience in design, management, and implementation of research projects to advance understanding of proteins, antibodies, bio- and pharmaceutical compounds. RESEARCH EXPERIENCE

Research Associate, University of Tennessee, Health Science Center (2009- present) Designed and led the research project (NIH and VA funded) using analytical, biochemical, cell biology and molecular science techniques to elucidate the role of specific proteins and unique vesicles in lipid absorption.

Selected Accomplishments:

Discovered a novel protein associated vesicle responsible for more than 80% of lipid absorption in intestine.

Discovered a novel protein kinase for drug target in intestinal fat absorption.

Identify and characterized the proteins and lipids using chromatography, HPLC, LCMS, MALDI-TOF, GC/ GCMS, cell biology and molecular science techniques.

Developed novel cell biological, molecular, biochemical and protein kinase assays.

Developed specific peptides for antibodies.

Developed and purified several antibodies such as Sar1B, Sar1A, VAMP-7, ApoB-48 etc.

Isolate and characterized the phospholipids from enterocytes.

Published and presented the research in high impact journals, national and international conferences.

Supervised and trained Post-doctoral fellow, students, and technicians on independent projects.

Collaborate the research projects with national and international research groups at Yale University, Rutgers University, Washington University, and Toronto University etc. Post-Doctoral Fellow, University of Tennessee, Health Science Center (2004-2009) Designed and led the NIH funded research projects to identify the proteins and lipids responsible for lipid absorption.

Selected Accomplishments:

Discovered a protein (FABP-1) responsible for the generation of pre-chylomicron transport vesicles (PCTV).

Identified and characterized the Vesicle-associated membrane protein 7 (VAMP7) using 2D gel electrophoresis, Mass spectroscopy, MALDI-TOF, LCMS and other molecular science techniques.

Synthesize the specific peptides for VAMP-7 and other proteins.

Developed and purified the antibodies against the VAMP-7 and other proteins.

Published and presented the research in high impact journals such as J Biol Chem, J Lipid Res. and J Cell Sci. etc.

Supervised and trained students, technicians and collaborators. Post-Doctoral Fellow, University of Graz, Austria. (2004-2004) Designed and led the microwave assisted organic synthesis project. Selected Accomplishment:

Synthesis of organic molecules for medicinal values using different monomode and multimode microwave synthesizer.

Identified and characterized the bio-chemicals using NMR, EI-MS, HPLC, FT-IR, UV, FAB-MS, HR-MS etc.

Supervised the undergraduate students, technicians and collaborate with interdepartmental research groups.

Post-Doctoral Fellow, Technical University Vienna, Austria. (2002-2004) Designed and led the projects towards the development of the pharmaceutical compounds in context of the Drug Matrix genomics projects.

Selected Accomplishments:

Developed an efficient and high yielding novel route towards the preparation of Ciglitazone and Troglitazone, antidiabetic drugs.

Developed a novel synthetic and cost efficient route towards the preparation of Amineptine atypical tricyclic anti-depressant with central nervous system stimulating effect.

Developed a novel synthesis for preparation of Lasofoxifene, is a selective estrogen receptor modulator (SERM) for the presentation and treatment of Osteoporosis.

Supervised and led the group of chemist, undergraduate students, technicians and collaborators.

Post-Doctoral Fellow, University of Vienna, Austria. (2001-2002) Designed and led the project for characterization and synthesis of Elisabethin A, a naturally occurring anticancer molecule.

Selected Accomplishments:

Synthesis of orthogonal protected phenols, a precursor for Elisabethin A.

Identified and characterized the organic molecules using HPLC, NMR, IR, UV, ESI- MS etc.

Supervised the students and technicians on individual projects.

Published novel finding in research articles in high impact and conferences. EDUCATION

Institution Degrees Year(s)

Central Institute of Medicinal Aromatic Plants, Lucknow, India.

(Dr. Ram Manohar Lohia Avadh University Faizabad, India.) Ph.D.

(Chemistry)

2001

Rohilkhand University, Bareilly, India

M.Sc.

(Org. Chemistry)

1996

Rohilkhand University, Bareilly, India B.Sc.

(Chemistry, Math)

1994

Laboratory Skills

Protein Biochemistry: Proteomics, proteolytic mapping, immunoprecipitation, far-western, enzyme kinetics, calorimetry (DSC), protein-protein interaction, Tryptophan fluorescence etc. Protein/ Peptide purification: Purification of proteins / polypeptides HPLC, FPLC, UPLC, column chromatography, ion exchange chromatography, affinity and size exclusion chromatography, electrophoresis etc.

Protein/ Peptide Identification: Identification of proteins using Mass spectroscopy, MALDI-TOF, LCMS, ESI-MS, Tandem MS-MS, EI-MS, NMR etc. Spectroscopic Techniques: Confident in using the spectroscopic instruments such as NMR, 2D-NMR, FT-IR, UV, FAB-MS, HR-MS, MALDI-TOF, LC-MS, Gas chromatography, GC-MS etc.

Molecular Biology: Western Blots, SDS-PAGE, capillary electrophoresis, CZE, CE-SDS, SEC-MALLS, CG-MALLS, Immunofluorescence, Immuno-purification, Immuno-precipitation, ELISA, Electron microscopy, Immuno-electron microscopy, 2D-Gel electrophoresis, Centrifugation Ultra centrifugation, dialysis, antibody production, particle sizing, protein assays, step and continuous gradient, radiolabeling of cell/ protein/lipid, northern/ southern blotting, lipid/ phopholipids identification and characterization etc. Computer/ Software: Proficiency with Windows and OSX operating systems. Highly competent in Microsoft Word, Excel, PowerPoint, Photoshop, Abode, Graph Pad Prism. Data Analysis/Statistics: Proficiency with Prism, Microsoft Excel, Origin and other commonly used analysis software.

Professional Affiliations

Member, Achievement Advisory Council (AAC; 2014-2015).

Member, The American Society for Biochemistry and Molecular Biology (2009-2010)

Member, The New York Academy of Sciences (2009-2010)

Member, The American Society for Cell Biology (2009-2010)

Member, Indian Chemical Society (1999-2000)

PUBLICATIONS

Research articles in journals:

1. Charles M. Mansbach II and Shahzad Siddiqi (2015) Control of Chylomicron Export from the Intestine. (Review) American Journal of Physiology: GI & Liver (In Press). 2. Shahzad Siddiqi and Charles M. Mansbach II (2015) Dietary and Biliary Phosphatidylcholine Activates PKCζ in Rat Intestine. J. Lipid Res., 56, 859-870. 3. Shahzad Siddiqi, Atur Sheth, Feenalie Patel, Matthew Barnes and Charles M. Mansbach II

(2013) Intestinal Caveolin-1 Is Important for Dietary Fatty Acid Absorption. BBA - Biochimica et Biophysica Acta; Molecular and Cell Biology of Lipids, 1831(8):1311-21. 4. Shahzad Siddiqi and Charles M. Mansbach II (2012) Phosphorylation of Sar1b Releases the Liver Fatty Acid Binding Protein from a Multi-protein Complex in Intestinal Cytosol Enabling It to Bind to the ER and Bud the Pre-chylomicron Transport Vesicle. J Biol Chem. 287(13):10178-88.

5. Shahzad Siddiqi, Umair Saleem, Nada Abumrad, Nicholas O. Davidson, Judith Storch, Shadab A. Siddiqi and Charles M. Mansbach II (2010) A novel multi-protein complex is required for the generation of the pre-chylomicron transport vesicle from intestinal ER. . J. Lipid Res. 51 (7), pp. 1918-1928.

6. Shahzad Siddiqi, Shadab A. Siddiqi, and Charles M. Mansbach II (2009). SEC24C is required for the docking of the pre-chylomicron transport vesicle with the Golgi. J. Lipid Res. 51 (5), pp. 1093-1100.

7. Indira Neeli, Shadab A. Siddiqi, Shahzad Siddiqi, William S. Lagakos, Bert Binas, Tarun Gheyi, Judith Storch, and Charles M. Mansbach II (2007) Liver fatty acid-binding protein initiates budding of pre-chylomicron transport vesicles from intestinal endoplasmic reticulum. J Biol Chem. 282(25):17974-84.

8. Shadab A. Siddiqi, Shahzad Siddiqi, James Mahan, Kiffany Peggs, Fred S. Gorelick and Charles M. Mansbach, II (2006), The identification of a novel endoplasmic reticulum to Golgi SNARE complex used by the prechylomicron transport vesicle. J. Biol. Chem.,Vol. 281(30), 20974-82.

9. Shadab A. Siddiqi, James Mahan, Shahzad Siddiqi, Fred S. Gorelick and Charles M. Mansbach, II (2006) Vesicle-associated membrane protein 7 is expressed in intestinal ER, J Cell Sci., 943-50.

10. Misra, L.N. and Siddiqi, S. A. (2005), Biologically active inositol, sterols and lipid derivatives from Sesbania bispinosa. Ind. J. of chemistry Sec. B, 44B(9), 1915-21. 11. L. N. Misra and S. A. Siddiqi (2004). Dhaincha (Sesbania bispinosa) leaves: A good source of antidiabetic -pinitol, Current Science, 1507. 12. S. A. Siddiqi and T. J. Heckrodt (2003). Access to Orthogonal Protected Phenols - Synthesis of a Silylated Quinol. Z.Naturforsch, 55b, 328-31. 13. Siddiqi, S.A. and Misra, L.N. (2000). Fatty acids and sterols from Cymbopogon martinii var. motia roots. Z.Naturforsch, 55c, 843-45.

14. Misra, L.N. and Siddiqi, S.A. (2000) n-Alkanes and alpha-palmitin from Paspalum scrobiculatum seeds. Z.Naturforsch, 55c, 500-502.

Symposia, Conferences and Invited Lectures:

1. Shahzad Siddiqi, Samantha Polly, Tabassum Siddiqi, Charles M. Mansbach II. Apolipoprotein-AV Resists Proteolysis in the Intestinal Tract and Is Absorbed Intact, Controlled by Dietary Phosphatidylcholine. Digestive Disease Week 2015, Washington DC, USA. Abstract: Gastroenterology, Vol. 148, Issue 4, Supplement 1, Pages S-880–S-881. 2. Samantha Polly, Shahzad Siddiqi, Charles M. Mansbach II. Apolipoprotein-AV: Enterohepatic Cycling of a Protein. Southern Society for Clinical investigation, 2015, New Orleans LA, USA.

3. Shahzad Siddiqi and Charles M. Mansbach II. Caveolin-1 containing Cytosolic Endocytic Vesicles (CEV) Are Important for Dietary Fatty Acid Absorption. Molecular Mechanisms of Instestinal Lipid Transport and Metabolism, FASEB, July 2014, Snowmass Village Colorado, USA.

4. Shahzad Siddiqi and Charles M. Mansbach II. Lyso-Phosphatidylcholine Is Important for Intestinal Fat Absorption. Digestive Disease Week 2014, Chicago, IL, USA. Abstract:: Gastroenterology, Vol. 146, Issue 5, S-492–S-493.

5. Shahzad Siddiqi and Charles M. Mansbach II. Caveolin-1 Containing Caveolae and Caveolin-1 containing Cytosolic Endocytic Vesicles Are Important for Dietary Fatty Acid Absorption. Digestive Disease Week 2013, Orlando, FL, USA. Abstract:: Gastroenterology Vol. 144, Issue 5, Supplement 1, Page S-162.

6. Shahzad Siddiqi and Charles M. Mansbach II. Budding of the Pre-chylomicron Transport Vesicle is Controlled by Phosphorylation of Sar1b. Digestive Disease Week 2012, San Diego CA, USA. Abstract ::Gastroenterology Vol. 142, Issue 5, Supplement 1, Pages S-198- S-199

7. Shahzad Siddiqi and Charles M. Mansbach II. Dietary Fatty Acid in Rat Intestinal Cytosol Is Associated with Caveolae. Digestive Disease Week 2011, Chicago IL, USA. Abstract :: Gastroenterology Vol. 140, Issue 5, Supplement 1, Pages S-542-S-543 8. Shahzad Siddiqi, Shadab A. Siddiqi, and Charles M. Mansbach II. A 10 Protein Complex is required to Bud the Pre-chylomicron Transport Vesicle (PCTV) from Rat Intestinal ER. Digestive Disease Week 2010, New Orleans LA, USA. Abstarct :: Gastroenterology Vol. 138, Issue 5, Supplement 1, Page S-606.

9. J. Skubak, B.H. Yousefí, K. More, S. Siddiqi, U. Jordis: "Aryl substituted Azabicycle- alkanes by palladium coupling to nitrogen"; XXI.European Colloquium on Heterocyclic Chemistry, Sopron, Ungar: "XXI. European Colloquium on Heterocyclic Chemistry - Book of Abstracts", (2004), S. 189.

10. Klaus Gerdes, Shahzad Siddiqi, Jaywant Phopase and Ulrich Jordis Synthesis of 7-(10, 11- Dihydro-5H-dibenzo[a,d]-cyclo-hepten-5-ylamino)-heptanoicacid (Amineptine) 8th International Electronic Conference on Synthetic Organic Chemistry. ECSOC-8. 1-30 November 2004.

11. Shahzad A. Siddiqi, (2004) Ciglitazone, a new hypoglycemic agent. Institute for Applied Chemistry, Technical University of Wien, Vienna Austria. 12. Shahzad A. Siddiqi, (2004) Thiazolidine-2,4-dione Group – A Good Source of Anti Diabetic Drug. Department of Chemistry, University of Graz, Graz Austria. 13. Shahzad A. Siddiqi, 10th Blue Danube Symposium on Heterocyclic Chemistry 3 – 6 September 2003, Vienna, Austria.

14. Thilo J. Heckrodt, S. A. Siddiqi, J. Mulzer “ Studies Towards a Diastereoselective Total Synthesis of Elisabethin A” Österreichische Chemietage 2002, Linz, Austria 15. Thilo J. Heckrodt, S. A. Siddiqi, J. Mulzer “Studies Towards a Diastereoselective Total Synthesis of Elisabethin A” ORCHEM 2002, Bad Nauheim, Germany 16. Misra, L.N. and Siddiqi, S.A., Recent advances in natural products research in modern medicines, National Seminar on Drug from Himalayan Herbs: Present status and future strategies, From March 26-28, 1999, Kumaun University, Nainital. 17. Siddiqi, S.A. and Misra, L.N., "Paspalum scrobiculatum seeds: A good source of n- alkanes", International conference on chemistry, Indian chemical society, 10-16 December 1999, Calcutta.



Contact this candidate