Jae Dugan

Detail orient Research Scientist and Career Development Award Recipient

Portland, OR

actcb9@r.postjobfree.com - 503-***-****

My career objective is to work for a biotech tech that will utilize my background in molecular biology and immunology. LinkedIn profile: https://www.linkedin.com/in/jaedugan Degrees Obtained

Willing to relocate: Anywhere

Authorized to work in the US for any employer

WORK EXPERIENCE

Career Development Award Recipient, VA Portland Healthcare Systems and Instructor, Department of Medicine, Oregon Health and Sciences University VA Portland Healthcare System - 2011 to Present

2011-Present

Instructor, Department of Medicine, Oregon Health and Sciences University Postdoctoral Research Fellow

VA Portland Health Care Systems - 2007 to 2011

Oregon Health and Sciences University

Advisor: Michael Davey

Research Topic: Role of NOD2 in Innate Immunity

Graduate Student

Oregon State University - 2001 to 2007

Advisor: Dan Rockey

Thesis Title: The analysis of tetC genomic islands present in tetracycline resistant Chlamydia suis: Using the genomic island to transform Chlamydia.

Skills

Supervision:

* 6 Research assistants.

Experience with small rodent models:

* Managed Mouse Colony

* Created Knock-in Mutant Mice where a specific point mutation was inserted into NOD2 in order to create a mouse model of a human inflammatory disorder (Blau syndrome)

* Dissection of organs for Histology and molecular analysis Protein Analysis and Purification:

* Established insect cell, bacterial, and tissue culture protein overexpression systems in order to obtain large amounts of target protein.

* Extensive experience with Western blot and 2D gel electrophoresis

* Experience with co-immunoprecipitation experiments to study protein interactions Molecular Skills:

* Sequenced over 20 kb of novel Chlamydia genome

* Experience subcloning and sequencing numerous genes of interest

* Experience with DNA and RNA purification systems

* Experience with PCR, RT-PCR, and Real Time PCR

* Experience with Southern and Northern blots

* Pulse Field Gel Electrophoresis

Immunology Skills:

* Bone Marrow Derived Macrophages and Dendritic Cells

* Flow cytometry

* Cytokine analysis

Growth of use of Microbial Organisms:

* Chlamydia species (Chlamydia trachomatis, Chlamydia suis, Chlamydia pneumonia)

* E. coli

* Candida albicans

* Listeria monocytogenes

* Aspergillus fumigatus

EDUCATION

Ph.D. in Molecular and Cellular Biology

Oregon State University

2007

B.S. in Biochemistry

University of Washington

2001

Chemistry

University of Washington

2001

PUBLICATIONS

Tetracycline Resistance in Chlamydia suis Mediated by Genomic Islands Inserted into the Chlamydial inv-like Gene

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC521927/ October 2004

Functional Characterization of IScs605, an insertion element carried by tetracycline- resistant Chlamydia suis

http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.29253-0 September 6, 2006

Nucleotide Oligomerization Domain-2 Interacts with 2’-5’-Oligoadenylate Synthetase Type 2 and Enhances RNaseL Function in THP-1 Cells http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787966/ December 1, 2010

Blau syndrome-associated Nod2 mutation alters expression of full-length NOD2 and limits responses to muramyl dipeptide in knock-in mice http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=25429073 January 1, 2015

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