Sneha Solanki

**** *. **** *****, ******* IL ****6

acskl5@r.postjobfree.com ۰-914-***-****

(US permanent resident)

Education:

Clark University, Worcester, Ma

Bachelor of Science, Biochemistry, Molecular Biology

Additional Coursework: Mammalian Toxicology, Histology, CPR certified

Work Experience:

University of Chicago, Hyde Park, IL June2015-present

Clinical Research Associate

Consented patients for phase 1 and melanoma clinical trials

Prepared pharmacokinetic study kits provided by the sponsor

Scheduled patient appointments using EPIC

Prepared Melanoma tumor board seminars

Collaborate with the Principal Investigators and other study team members

Assist with data tracking and management

Obtained clinical hours

Coordinated site initiation visits for sponsors to set up trials at the institution

Generate patient list for future weeks, documents all patients interactions on study trial

Make charts for patients enrolled in studies

Conducted meetings with site monitors for data management/database deadlines

Transport specimens/biopsies to appropriate laboratory for FFPE/slide processing

University of Chicago, Hyde Park, IL August 2014- Feb2015

Research Specialist in the Urology Lab

Maintained multiple cell lines (prostate and blood)

Drug experiments using Enz-LNCap cells. Used cells to detect MKP-1 protein at endogenous levels and to see if drug induced protein production

Injected mice via IP and IC with prostate lines maintained

Performed western blot on bladder lines to detect for SOX2 protein

Attempted TOPO cloning on MEIS1 protein

Worked with other lab members, presented materials

Updated and initiated writing of protocols

***Completed a class on Drug Processes and Drug development. One of five requirements for the Clinical Research Management certification.

Syros Pharmaceuticals, Watertown, Ma June 2013-August 2014

Research Associate II

Maintained multiple cell lines (blood and breast lines)

Optimized Chromatin Immunoprecipitation followed by Sequencing (Chip-Seq) in blood, breast cell lines and fresh frozen breast patient tumor samples (ER positive/PR positive/triple negative). Generated multiple datasets for the epigenetic marker H3K27ac.

Performed qPCR (using primers ECR1, MALAT1, RPL23) and DNA aragose gels as quality control

Performed routine drug screens using luminescence ATP-lite read out, used GraphPad to analyse.

Set up dosing experiments in several blood and breast cancer cells lines with HDAC and HAT inhibitors, varying day treatments, performed Western blots to confirm the affects

Under went CRISPR experiments. Purchased linear vector ligated with a custom designed oligo, cloned, mini-prep, sent out for sequencing. Maxi prep successful samples and transduced vector into breast cancer cells

Normalization of ChIP-seq experiment with drug with S2 drosophila cells in Jurkat and MV4;11 cells at varying concentrations

Smartflare RNA detection in AML cells lines

Biomedical Tracers seminar + Lab, UMass Boston, Ma January 2013-May 2013

Graduate level course, describing the types and uses of physical, chemical, & biochemical tracers in the biomedical sciences

Harvard School of Public Health, Boston, Ma May 2012-September 2012

Research Associate (Assay Development), Department of Nutrition and Genetics

Developed an assay to measure cholesterol in lipoprotein subfractions for clinic testing

Implemented different methods to isolate cholesterol from the lipoprotein mass from patient plasma

Purified ApoA1 by immunoaffinity chromatography

Quantified ApoA1 by ELISA analysis

Assessed protein purity and structural integrity by Native SDS PAGE gels 2D and Western Blot analysis

Prepared buffers and solutions required for procedures

Maintained lab notebook, Data entry

Newman School, Boston, Ma September 2011-March 2012

Laboratory Coordinator

Organized laboratories and conducted lessons for 9th, 11th and 12th graders

Collaborated with faculty members to enhance the biology curriculum

Cubist Pharmaceuticals, Lexington, Ma May 2011-August 2011

Research Associate, Toxicology/Non-Clinical Development

Maintained cells lines (HK2-human kidney2, A431- epidermoid carcinoma, and hERG transfected HEK293-human embryonic kidney cell lines) that were used in sophisticated cell-based assays to screen candidate drug compounds

Conducted membrane and saturation assays using the hERG cell lines

Performed lactate dehydrogenase (LDH)- and adenosine triphosphate (ATP)-based cytotoxicity assays

Utilized [3H]dofetilide as a binding assay to screen for hERG K+ channel

Analyzed data and ensured quality control

Nuclea Biotechnologies, Worcester, Ma May 2010-May 2011

Research Associate

Performed ELISA on specific cardiac markers, BNP, KCNE2, GSTomega1, SOD2 and breast markers, TACC3, HACP-G. Using the sandwich method a matrix system was set up for each marker for a monoclonal capture-polyclonal detection frame. These markers were run against blood serum to test levels of the markers in the patients sample, to diagnose/treat patients

Quality controlled DNA/RNA using Flash Gel technology, checked concentration levels of DNA/RNA present in a sample using the NanoDrop

MiniPreps used for DNA/RNA extraction from the maintained cell lines

Ran qPCR on NCI 60 cell lines checking for mycoplasma bacteria, SDS PAGE 2D gel and flash gel

Maintained NCI 60 cell lines: Adherent and suspension cultures using aseptic technique

Performed necropsies on mice after injection of cancerous cells. Tumours excised and treated with chemotherapeutic drugs

Tissue sections cut with microtome stained with Haematoxylin and Eosin

Clark University, Cell and molecular Biology Lab September 2008-May 2010

Light Microscopy

Maintained primary cell cultures from vertebrate organisms, mainly mice using aseptic technique

Cultures of lung cell macrophages from mice served as animal models for the Hermansky-Pudlak syndrome. Performed qPCR for bacteria

Performed mice surgeries to remove primary lung cells

Performed protein assays; Bradford, BCA, ELISA assays

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