Maria Kokkinaki

**** ****** ******* **.,

Apt ***2, Herndon, VA 20171

E-mail: acsgvz@r.postjobfree.com

Phone: 202-***-**** (cell)

Recent positions

Post doctoral research fellow, Georgetown University Washington DC: April 2006-June 2015

Freelance Editor for scientific manuscripts, CACTUS Communications, May 2015-present

Education

PhD in Molecular Biology & Human Molecular Genetics, 2005 / M.SC in Molecular Biology & Biotechnology, 1996 / B.SC in Biology, 1994: University of Crete, Heraklion, Greece

Postdoc training in Human Molecular Genetics, 2005-2006: University of Crete, Heraklion, Greece

Postdoc training in Spermatogonial Stem Cells and Induced Pluripotent Stem Cells, 2006-2009: Georgetown University, Washington DC, USA

Immigration status

Permanent resident of the USA

Citizen of Greece

Accomplishments - Awards

PhD and Master theses in Human Molecular Genetics

7 years of research in Cell and Molecular biology

14 research papers published in peer-reviewed scientific journals during the years 2009 to 2014. First authorship in 5 of them (list of publications will be provided upon request)

Advisor to 6 graduate internship students, 1 PhD student and 3 Master students

Awarded with the Certificate of Appreciation 2015 from the Departments of Ophthalmology, Neurology and Biochemistry of Georgetown University for training students in the M.S. Biochemistry and Molecular Biology Program

Areas of Expertise

LAB MANAGEMENT

Responsible for procurement of lab equipment and reagents

Responsible for the lab administration and budget

Responsible for lab safety

RESEARCH

• Perform experiments to pursue goals of assigned research projects

Disease modeling using induced pluripotent stem cells

Single Nucleotide Polymorphism genotyping

TECHNICAL SKILLS

Molecular Biology: Nucleic acid and protein extraction and purification, Western blotting, ELISA, oligo design, PCR and real-time RT-PCR, cloning and sub-cloning, microarray gene expression analysis, Next Gen Sequencing, gel electrophoresis, immunohistochemistry, immunofluorescence, gene cloning, bacterial and yeast cultures

Cell Culture: Mammalian cell culture, bacterial cell culture, media preparation, cell-based assays, enzyme linked immunoassays, transfection, transduction with lentiviruses, retroviruses, or adenoviruses, viral quantification assays (fluorescent focus forming and plaque assay), confocal microscopy

Animal studies: Regular care of laboratory mice or rats, breeding, survival and non-survival surgery, genotyping

WRITING SKILLS

• Write and edit manuscripts for publications in scientific journals

Write and edit grant proposals

Write protocols and SOPs

TEACHING

Advisor to graduate and postgraduate students in Georgetown University (2007-2015), offering the necessary training for them to accomplish their research projects for their theses

Adjunct professor of Human Genetics in the Dpt of Biology, University of Crete, (2004-2006), taught Human genetics course and laboratory techniques to 3rd year undergraduate students of Biology.

SOFTWARE AND COMPUTING SKILLS

Proficiency in:

Genomic and proteomic databases (GenBank, PDB, Ingenuity IPA analysis, Panther, KEGG)

Statistical software (STATA)

Document and image processing software (e.g. Microsoft Office, Photoshop, Image J)

SPECIAL SKILLS

Languages spoken:

English: fluently (second language)

Greek: Native speaker

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